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A kind of preparation method of Indian piriformis stalk culture medium for cultivating shiitake mushrooms

A technology for preparation of piriformis sp. indica and culture medium, which is applied in the field of microorganisms, can solve the problem of imprecise promotion mechanism research, and achieve the effect of increasing the yield of shiitake mushrooms and promoting the growth of shiitake mushroom roots

Active Publication Date: 2020-07-03
YANGTZE UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] How to apply the microbial culture medium to the cultivation of shiitake mushrooms is a widely researched issue. The existing microbial culture medium also has its own shortcomings, such as the long-term activity of the microbial culture medium and the imprecise research on the promotion mechanism.

Method used

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  • A kind of preparation method of Indian piriformis stalk culture medium for cultivating shiitake mushrooms
  • A kind of preparation method of Indian piriformis stalk culture medium for cultivating shiitake mushrooms
  • A kind of preparation method of Indian piriformis stalk culture medium for cultivating shiitake mushrooms

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preparation example Construction

[0011] The first aspect of the present invention provides a method for preparing a culture medium of Piriformis indica stalks for cultivating shiitake mushrooms, the steps comprising:

[0012] (1) Inoculate Pyromorpha indica into Kafer medium, rotate at a speed of 120-150r / min, place it in a rotary constant temperature and speed-regulating shaker cabinet at 26-30°C for shaking culture for 6-8 days, and take samples to determine the number of viable bacteria at the end of the culture. And the culture was dissolved in distilled water so that the number of viable bacteria per ml solution was 1×10 6 indivual;

[0013] (2) The number of bacteria obtained in step (1) is 1×10 6 The bacterium liquid per mL is sprayed on the surface of the stalk culture medium according to the proportion of 1-15% of the inoculation amount, and fermented at 25-37 DEG C for 12-24 hours to obtain the Pyromorpha indica straw culture medium for cultivating shiitake mushrooms.

[0014] Preferably, the form...

Embodiment 1

[0024] This example provides a preparation method of Pyromorpha indica stalk medium for cultivating Lentinus edodes, which is used for cultivating Lentinus edodes, and the fermentation time in the preparation process is screened.

[0025] The composition of the kafer medium used in this example can adopt the conventional formula in the art.

[0026] The microorganism Pyromorpha indica used was provided by the Institute of Biomedicine of Yangtze University, and the sources of microorganisms are not limited to this. Similar effects can be obtained from Pyromorpha indica purchased or isolated from the market.

[0027] The formula of the straw medium used is: 62% of corn straw powder, 26% of sawdust, 10% of wheat bran, 1% of sucrose, and 1% of gypsum.

[0028] A method for preparing a stalk medium of Piriformis indica for cultivating shiitake mushrooms, the steps comprising:

[0029] (1) Inoculate Pyromorpha indica into Kafer medium, place in a rotary shaker flask with constant t...

Embodiment 2

[0038] This example provides a preparation method of Pyromorpha indica stalk medium for cultivating Lentinus edodes, which is used for cultivating Lentinus edodes, and the optimal inoculation amount of strains in the preparation process is screened.

[0039] (1) According to the results of Example 1, the fixed fermentation time was 18 hours, and the formula of the medium was changed through a single factor test, and the specific formula was shown in Table 2.

[0040] Table 2 The formula of Pyromorpha stalk medium in India

[0041]

[0042] The raw materials are crushed according to the formula of the straw medium, and mixed to prepare the straw medium for cultivation.

[0043] (2) Change the formula of culture medium by single factor test, according to the test plan of table 2, the number of viable bacteria is 1 * 10 6Each / mL of the bacterial solution was inoculated into the straw medium, the inoculum amounts were 1%, 5%, 10%, 15% (V / g), placed at 34°C for 24h, and the cul...

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Abstract

The invention provides a preparation method for a Piriformospora indica-straw medium used for culturing mushrooms. The preparation method comprises the following steps: (1) inoculating a Kafer medium with Piriformospora indica, carrying out shaking culture at a rotating speed of 120 to 150 r / min and a temperature of 26 to 30 DEG C for 6 to 8 d, carrying out sampling after completion of culture, determining the number of live bacteria and dissolving a culture in distilled water so as to allow the number of live bacteria in every ml of the obtained solution to be 1*10<6>; and (2) sprinkling bacteria liquid obtained in the step (1) and having a bacterium number of 1*10<6> / ml onto the surface of a straw medium, with the inoculation amount of the bacteria liquid being 1 to 15%, and carrying out fermentation at 25 to 37 DEG C for 12 to 24 h so as to obtain the Piriformospora indica-straw medium used for culturing mushrooms. According to the invention, Piriformospora indica is added, and optimal cultivation conditions are selected, so the growth of mushroom rootlets is promoted; and thus, the output of mushrooms is increased.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for preparing a piriformis stalk culture medium for cultivating shiitake mushrooms. Background technique [0002] Mushroom cultivation originated in China and has a history of more than 800 years. Wu Sangong, a farmer in Longyan Village, Qingyuan County, Zhejiang Province, first invented the cutting flower cultivation method in the Song Dynasty, and it spread throughout the country. It was introduced to Japan through the exchanges of monks. The cut flower cultivation of shiitake mushrooms originated in China, and the current inoculation cultivation of Duanmu pure mycelium originated in Japan. By 1989, the total production of shiitake mushrooms in China surpassed that of Japan for the first time, becoming the world's largest shiitake mushroom production country. [0003] Pyromorpha indica is a mycorrhizal fungus that can be cultured purely. The fungus colo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G5/40C05F17/00A01G18/20
CPCC05D3/00C05F17/00C05G3/00C05F5/002C05F11/00C05F11/08Y02W30/40
Inventor 熊涛孔德乾吴楚君
Owner YANGTZE UNIVERSITY
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