Method for reducing cadmium content in tobacco leaves

A content, tobacco leaf technology, applied in the field of microbial resource utilization and agricultural product quality and safety, can solve problems such as unfavorable large-scale popularization and application, arbuscular mycorrhizal fungi cannot grow, etc., and achieves no secondary pollution, low cost, and reduction of chemical fertilizers. the effect of using

Inactive Publication Date: 2014-07-02
CHINA TOBACCO GUIZHOU IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, arbuscular mycorrhizal fungi cannot grow on synthetic media, which is not conducive to the large-scale application of the fungus.

Method used

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  • Method for reducing cadmium content in tobacco leaves
  • Method for reducing cadmium content in tobacco leaves
  • Method for reducing cadmium content in tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for reducing the cadmium content of tobacco leaves, using the parasitic Pyromorpha indica to reduce tobacco heavy metal cadmium residues in tobacco roots, comprising the following steps:

[0040] Activation of strains: Inoculate the Pyromorpha indica strains stored in the refrigerator on Kafer medium (KM) plates, and culture them in a 28°C incubator in the dark for 7 days. Use a hole punch with a diameter of 5 mm to punch holes at the edge of the activated Pyrocytosporum indica colony.

[0041] The cultivation of bacterial liquid: prepare liquid Kafer culture medium and the liquid Kafer culture medium that contains nanomaterial ZnO (30nm) 0.01g / L (that is to replace ZnSO with the nanomaterial ZnO of particle size 30nm) 4 ·7H 2 O), use 250mL conical flasks to pack, each bottle contains 150mL culture medium, and sterilize for later use. Under sterile conditions, add the mycelia blocks of Pyromorpha indica into the Erlenmeyer flask containing the above medium, 6...

Embodiment 2

[0061] In Example 1, the mycelia block of Pyriformis indica was changed into Pyromorpha indica spore liquid, inoculated in the liquid Kafer medium containing 10mg / LZnO, and cultured in a constant temperature shaker at 120rpm and 25°C in the dark for 7 days, and the rest Identical to Example 1, the final cadmium content in hydroponic, potted and field tobacco leaves was reduced by 46.2%, 38.9%, and 51.8%, respectively.

Embodiment 3

[0063] In Example 1, the concentration of Pyromorpha indica mycelia liquid was changed to 2g / L, and the rest were equal to Example 1. Finally, the content of cadmium in hydroponics, potted plants and field tobacco leaves was respectively reduced by 49.2%, 48.5%, and 47.5%.

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Abstract

Provided is a method for reducing the cadmium content in tobacco leaves. The method comprises the specific steps that (1) nanometer material zinc oxide is used for replacing ZnSO4.7H2O in original Kafer culture media, and 10 mg of nanometer material zinc oxide is added into 1 liter of culture media; (2) activated Piriformospora indica is inoculated to the liquid Kafer culture media in the step (1) for culture, then mycelia are collected, Piriformospora indica mycelium liquid is made and poured to roots of tobacco seedlings, and root-irrigation is conducted so that the Piriformospora indica can be colonized to the roots of the tobacco seedlings; (3) the tobacco seedlings are transplanted after the Piriformospora indica is colonized, and conventional water and fertilizer management are conducted so that the tobacco seedlings can grow normally. The growth status of the tobacco seedlings can be improved in the method, the amount of cadmium moving to overground parts of the tobacco seedlings from root systems is reduced, heavy metal residue on the tobacco leaves is reduced, and the method is easy to master, low in cost, simple, practical and environmentally friendly, and can not case secondary pollution.

Description

technical field [0001] The invention relates to the technical field of utilization of microbial resources and quality and safety of agricultural products, in particular to a method for reducing cadmium content in tobacco leaves. Background technique [0002] With the extensive use of chemical fertilizers and pesticides and the rapid development of industry, environmental pollution has become increasingly serious. Cadmium is a toxic heavy metal and one of the major environmental pollutants. Tobacco is one of the important economic crops in my country. The planting area, flue-cured tobacco output and production volume of China rank first in the world. In recent years, there have been reports of quality and safety issues such as excessive heavy metals in tobacco, among which cadmium, chromium, and lead have a more serious impact on tobacco growth and quality. Cadmium and lead in the soil can inhibit the growth of tobacco, change the morphology, physiology, biochemistry and st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/06C12N1/38C12N1/14C12R1/645
Inventor 聂长春刘剑惠建权刘孜王玉平毛寒冰楼兵干惠非琼刘晓曦高其康林福呈
Owner CHINA TOBACCO GUIZHOU IND
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