Piriformospora indica fermentation method and response surface optimization method used by same
A technology of Pyromorpha indica and fermentation method, which is applied in the field of secondary metabolite fermentation production process optimization, can solve problems such as the determination of the composition and structure of secondary metabolites, the unfavorable industrial production of endophytic fungi and agricultural application, etc., to achieve The effect of improving fermentation production yield, high use value and reducing energy consumption
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Embodiment 1
[0056] Embodiment 1, a kind of Indian piriformis fermentation optimization method comprises the steps:
[0057] Inoculate Pyromorpha indica on PDA plates and activate at 28°C for 14 days. Use a 5mm hole puncher to punch holes on the edge of the activated Piriformis indica flat plate.
[0058] Adjust 300 mL of PDB medium (stock solution) to pH = 5 with 1 mol / L HCl solution, then put the 5 round cakes obtained by punching holes and incubate at 28°C for 14 days.
[0059] Filtrate the piriformis indica fermentation liquid obtained by culturing for 14 days with gauze, mix the filtrate with 50 ml of ethyl acetate in a 1 L separatory funnel, leave it for 2 h, separate the organic phase and the aqueous phase, and extract a total of 3 times; Dry the organic phase with anhydrous sodium sulfate, then concentrate and evaporate to dryness at a temperature of 40-41°C, then dissolve it in 10 mL of absolute ethanol, dilute 100 times with absolute ethanol, take 1 mL of the diluted solution an...
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