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Piriformospora indica microbial agent and preparation method thereof

A technology of Pyromorpha spp. and bacterial agent, which is applied in the field of Pyromorpha spp. and its preparation, can solve the problems of expanding cultivation barriers, being easily polluted by miscellaneous bacteria, and slow growth, so as to advance the spore production time and solve the problem of pesticides. Residual, growth-promoting effect

Active Publication Date: 2022-03-01
天津市农业科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few reports on the large-scale promotion and application of Piriformis indica as bacterial agents in agriculture, because the growth of Pyromorpha indica itself is slow, and it is easily contaminated by miscellaneous bacteria during the growth process, which causes obstacles to expanded cultivation. The number of chlamydospores produced by Piriformis indica on ordinary medium is relatively small, and as a plant endophyte, Piriformis indica mainly relies on chlamydospores to colonize the plant rhizosphere and enter the plant to exert its role in inducing resistance

Method used

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  • Piriformospora indica microbial agent and preparation method thereof
  • Piriformospora indica microbial agent and preparation method thereof
  • Piriformospora indica microbial agent and preparation method thereof

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Effect test

Embodiment 1

[0033] 1. Activation of bacteria

[0034] The Indian Pyromorpha strain L-L-109 that the present invention uses adopts the Indian Pyromorpha strain L-L-109 that has been preserved in the General Microbiology Center (abbreviated as CGMCC) of China Microorganism Preservation Management Committee in January, 2015:

[0035] The address of the preservation unit is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing;

[0036] Date of preservation: January 26, 2015;

[0037] The deposit number is: CGMCC NO.10325,

[0038] The taxonomic name is: Piriformospora indica.

[0039] The Pyromorpha indica strain L-L-109 has been disclosed in Chinese patent application CN201610540885.3.

[0040] Place Pyromorpha indica L-L-109 in a PDA (potato dextrose agar medium) medium and culture it at 24-26° C. for 15 days. The PDA medium consists of: 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of tap water, and natural pH.

[0041] Cultured for 7 days ( figu...

Embodiment 2

[0052] Embodiment 2, preparation Indian piriformis agent

[0053] Follow the steps below:

[0054] 1) Strain activation: the same as the strain activation method in Example 1.

[0055] 2) Seed solution preparation: adopt the improved CM liquid medium in Example 1, the carbon source is soluble starch, adopt the steps of the seed solution preparation method in Example 1, shake culture (160r / min) at 28°C for 10 days, and cultivate Afterwards, the seed liquid of Piriformis indica was obtained.

[0056] 3) Solid fermentation culture: 5 ml of the seed solution obtained in step 2) was added to 300 ml of solid medium, and cultured at 24-26° C. (25° C. was specifically used in this embodiment) for 7 days. The solid medium is: add 5% (mass percentage of sucrose / sorghum grains) sucrose to the sorghum grains, add water to boil, the amount of water added is 20%-40% (w / w, weight ratio of water / sorghum grains) , until the water is fully absorbed, then put into 500ml Erlenmeyer flasks, 300...

Embodiment 3

[0059] Embodiment 3, the influence of Indian Pyrocytosporum agent on wheat

[0060] The wheat variety was Zhongmai 100, which was sown on October 9, 2020 at the Modern Agricultural Innovation Base of Tianjin Academy of Agricultural Sciences.

[0061] Treatment 1: Soak the seeds with the Pyromorpha indica preparation prepared in Example 2, the proportion of which is 1 gram of the bacterium plus 100 ml of tap water and mix evenly, soak the wheat seeds for 1 hour, remove and control the water, and then sow and cover the soil;

[0062] Process 2: pour in the sowing ditch with the Indian Pyromorpha bacterium liquid (adding water to make suspension with the Indian Pyromorpha bacterium agent prepared in embodiment 2, 1 gram of the bacterium agent adding 100ml tap water mixing and obtaining, being called for short the bacterium liquid), The sowing ditch was 1m long and 5cm wide, and 50ml of bacterial solution was poured per meter length, and then sowed and covered with soil; treatment...

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Abstract

The invention discloses a piriformospora indica microbial inoculum and a preparation method thereof, the piriformospora indica microbial inoculum comprises a fermentation carrier containing piriformospora indica thalli as an active ingredient, a spore protective agent, a dispersing agent and a spreading agent, the content of piriformospora indica chlamydospore in the fermentation carrier is (1.0-20.0) * 10 < 6 > spores / g, the weight percentage of the dispersing agent and the spreading agent is 1-5%, and the weight percentage of the spreading agent is 1-5%. The weight percentage of the spore protective agent is 0.05 to 0.1 percent. The method successfully solves the problem that piriformospora indica is difficult in spore production, large-scale application and long-term storage, when the seed solution is prepared, soluble starch is adopted as a carbon source, compared with other carbon sources, the growth speed of piriformospora indica hyphae can be remarkably increased, the spore production time is remarkably shortened, the spore production quantity is increased, and the production cost is reduced. Through solid culture with sorghum and cane sugar as fermentation carriers, the storability is improved, the prepared microbial inoculum can be preserved for a long time, the spore activity can be kept, and possibility is provided for large-area production and application.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to a piriformis sp. indica agent and a preparation method thereof. Background technique [0002] In agriculture, the use of pesticides has caused great pollution to the ecological environment, not only to other organisms but also to human beings themselves. Therefore, people began to continuously explore a sustainable green agricultural system, and gradually used agricultural probiotics, antibiotics, etc. to prevent and control plant diseases to replace the use of pesticides in the past. [0003] P. indica was first discovered by Indian scientists in the Thar Desert in Northwest India in 1998. P. indica is an endophytic fungus on plant roots, belonging to Basidiomycota, Phytomycetes, and Wax Shell Ears , Piromorpha sp. Its advantage is that it can grow on artificial medium and can successfully complete its life cycle, asexual reproduction forms chlamydospores containi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12N1/04A01N63/30A01P3/00A01P21/00C12R1/645
CPCC12N1/14C12N3/00C12N1/04A01N63/30Y02A50/30
Inventor 杨秀荣李亮孙淑琴李月娇李广胜
Owner 天津市农业科学院
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