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Method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil

A technology of Pyromorpha indica and Medicago sativa, applied in the field of biological application in environmental biology science, can solve the problem of lack of in-depth and systematic research on plant species selection, microbial species selection and bioremediation mechanism, difficult seed coating technology, root nodules Bacteria can not complete the growth and other problems, to achieve the effect of rapid and reliable method, improved tolerance and low cost

Inactive Publication Date: 2016-12-07
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method can reduce harmful pollutants in the soil, but it also reduces the multiple cropping index of cultivated land, and how to deal with host plants in practice is also a production problem
Chinese patent application 201210273541 discloses a multi-means combined method for remediating oil-contaminated soil in saline-alkali land. The inventive method utilizes alfalfa seeds coated with bacterial agents containing petroleum-degrading bacteria and alfalfa rhizobium, and the coated alfalfa seeds and The low-temperature-treated Leymus chinensis seeds were mixed, and the mixed seeds were sown in oil-contaminated soil in saline-alkaline land, but the preparation of petroleum inoculum and the coating of seeds were technically difficult, and the rhizobia could not complete the growth on the synthetic medium. Facilitate the large-scale popularization and application of the bacteria
[0006] At present, there are still few effective cases of using the interaction between rhizosphere microorganisms and plants to improve polluted soil at home and abroad, especially in the selection of plant species, microbial species selection and bioremediation mechanism. There is still a lack of in-depth and systematic research.

Method used

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  • Method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil
  • Method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil
  • Method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil

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Experimental program
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Effect test

Embodiment 1

[0031] The method of remediating petroleum-contaminated soil with the combined action of alfalfa-Piriformis india includes the following steps:

[0032] (1) Preparation of Piriformis indica culture medium: 20g glucose, 2g peptone, 1g yeast extract, 1g hydrolyzed casein, 20× salt solution: 50mL, 1mL trace element solution, add water to dissolve to 1L, and add 15g agar Powder, autoclaved, and poured into the plate after cooling is the solid CM medium.

[0033] The preparation method of 20× salt solution is: add 120g NaNO 3 , 10.4g KCl, 10.4g MgSO 4 ·7H 2 O and 30.4g KH 2 PO 4 Put it into the container and add sterile water to 1L.

[0034] The preparation method of the trace element solution is: 6g MnCl 2 ·4H 2 O, 1.5g H 3 BO 3 , 2.65g ZnSO 4 ·7H 2 O, 750mg KI, 2.4mg Na 2 MO 4 ·7H 2 O and 130mg CuSO 4 ·5H 2 Put O into the container and add sterile water to 1L.

[0035] (2) Activation of strains: Inoculate the pyriformis indica strains stored in the refrigerator on the solid CM medium obt...

Embodiment 2

[0047] After the fifth watering of Piriformis indica is completed, the soil enzyme activity is measured after 4 weeks. The determination of invertase, catalase, urease and alkaline phosphatase reflects the effect of the addition of Piriformis india on the soil. The steps are as follows:

[0048] (1) Sucrase: After measuring the standard curve, weigh 5g soil sample, place it in a 50mL Erlenmeyer flask, and inject 15mL 8% sucrose solution, 5mL pH5.5 phosphate buffer and 5 drops of toluene. After shaking the mixture, put it into a thermostat and incubate at 37°C for 24h. At that time, take it out and centrifuge at 6000rpm for 10min. Take 1.0 mL of supernatant (the volume of supernatant drawn from fresh soil samples is 1.0 mL; the volume of supernatant drawn from air-dried soil and soil samples stored for 1 month is 0.5 mL) in a 25 mL colorimetric tube, then Perform colorimetric determination according to the method of drawing standard curve color development. To eliminate the err...

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Abstract

The invention discloses a method of using joint action of medicago sativa and piriformospora indica to restore petroleum-contaminated soil. The method includes following steps: (1), culturing piriformospora indica, and collecting spore suspension; (2), cultivating seeds of medicago sativa; (3), inoculating piriformospora indica to medicago sativa. The piriformospora indica spore suspension is adopted to irrigate roots of medicago sativa, piriformospora indica is planted at the roots of medicago sativa, mycorrhizal symbionts formed by plants and endophytic fungi can promote growing of medicago sativa, bioactivity and fertility of soil are improved, petroleum in the soil can be effectively degraded, and degradation rate can reach 78%.

Description

Technical field [0001] The invention relates to biological application technology in environmental biological sciences, in particular to a method for applying the combined action of alfalfa and pyriformes india to repair soil oil pollution. Background technique [0002] The residual and accumulated petroleum pollutants in the environment not only seriously endanger the production function of the soil and the quality of agricultural products, but also pose a great threat to human health and the safety of the ecosystem. Bioremediation has become one of the most promising ways to treat oil-contaminated soil due to its simple operation technology, low cost, good repair effect and no secondary pollution. Among them, plants can promote the degradation of petroleum pollutants by soil microorganisms through the rhizosphere effect. Therefore, the joint restoration of plants and microorganisms can significantly improve the treatment effect. [0003] In petroleum-contaminated soil, if there...

Claims

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Application Information

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IPC IPC(8): A01B79/02
CPCA01B79/02
Inventor 李亮陈希马朝阳齐树亭王奋
Owner HEBEI UNIV OF TECH
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