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Method for promoting rooting and root strengthening of banana tissue culture seedlings

A technology for tissue culture seedlings and bananas, which is applied in the field of promoting the rooting and strengthening of banana tissue culture seedlings, and achieves the effects of simple operation, shortening the rooting cycle, and promoting rooting.

Inactive Publication Date: 2017-09-08
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that Pyriformis indica can significantly promote the formation, growth and rooting number of roots of various plants. Unfortunately, there are no reports of its application in the production of bananas, especially banana tissue culture seedlings.

Method used

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  • Method for promoting rooting and root strengthening of banana tissue culture seedlings
  • Method for promoting rooting and root strengthening of banana tissue culture seedlings
  • Method for promoting rooting and root strengthening of banana tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Cultivation of Piriformis indica

[0029] Wash and peel the potatoes and weigh 200 g, cut into small pieces or strips, add 800ml of distilled water into the pot and boil for 20-30min until the potatoes are cooked, filter through 4 layers of gauze, weigh 20g of glucose and 20g of agar respectively Add to the filtrate, distilled water to 1 L and boil to dissolve, then pack it into a Erlenmeyer flask for high-pressure steam sterilization (121°C, 20 min), pour it into a petri dish and seal it for later use, and make it into a PDA solid culture base.

[0030] To activate the Pyromorpha indica strain, use a hole puncher to take out the bacterial block with a diameter of 5 mm at the edge of the colony, insert it into the middle of the PDA solid medium plate, and incubate it in the dark at 28°C for 7 days before use.

[0031] 2, the preparation of banana tissue culture seedling rooting medium

[0032] The formula of rooting medium is MS+0.3 mg / L NAA+30 g / L sucrose+6 g / L ag...

Embodiment 2

[0041] 1. Cultivation of Piriformis indica

[0042] Wash and peel the potatoes, weigh 200 g, cut into small pieces or strips, add 800 ml of distilled water into the pot and boil for 20-30 min until the potatoes are cooked, filter through 4 layers of gauze, weigh 20 g of glucose and 20 g of Agar was added to the filtrate respectively, distilled water was adjusted to 1 L and boiled to dissolve, then packed into triangular flasks for high-pressure steam sterilization (121°C, 20min), after cooling slightly, pour them into petri dishes and seal them for later use, and prepare PDA solid media.

[0043] To activate the Pyromorpha indica strain, use a hole puncher to take out the bacterial block with a diameter of 5 mm at the edge of the colony, insert it into the middle of the PDA solid medium plate, and incubate it in the dark at 28°C for 7 days before use.

[0044] 2, the preparation of banana tissue culture seedling rooting medium

[0045] The formula of rooting medium is MS+0.3...

Embodiment 3

[0054] 1. Cultivation of Piriformis indica

[0055] Wash and peel the potatoes and weigh 200 g, cut into small pieces or strips, add 800ml of distilled water into the pot and boil for 20-30min until the potatoes are cooked, filter through 4 layers of gauze, weigh 20g of glucose and 20g of agar respectively Add to the filtrate, distilled water to 1 L and boil to dissolve, then pack it into a Erlenmeyer flask for high-pressure steam sterilization (121°C, 20 min), pour it into a petri dish and seal it for later use, and make it into a PDA solid culture base.

[0056] To activate the Pyromorpha indica strain, use a hole puncher to take out the bacterial block with a diameter of 5 mm at the edge of the colony, insert it into the middle of the PDA solid medium plate, and incubate it in the dark at 28°C for 7 days before use.

[0057] 2, the preparation of banana tissue culture seedling rooting medium

[0058] The formula of rooting medium is MS+0.3 mg / L NAA+30 g / L sucrose+6 g / L ag...

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Abstract

The invention provides a method for promoting rooting and root strengthening of banana tissue culture seedlings. The method comprises the following steps: cultivation of Piriformospora indica, preparation of a banana rooting medium, inoculation for co-culture of Piriformospora indica and banana tissue culture seedlings, and the like. Colonization of Piriformospora indica is used for promoting rooting of banana tissue culture seedlings, the method has simple operation, rooting period of tissue culture seedlings is shortened, and the method can substantially promote rooting of the banana tissue culture seedlings with a certain root strengthening effect. The method has good operationality and substantial effects.

Description

technical field [0001] The invention relates to the field of banana tissue culture, in particular to a method for promoting rooting and strengthening of banana tissue culture seedlings. Background technique [0002] Banana is the second largest fruit and the fourth largest food crop in the world. my country is an important origin of bananas, with a planting area of ​​6.2 million mu and an annual output of more than 10 million tons. It is the second largest banana producer in the world. The banana industry has become an important agricultural pillar industry in South China. Compared with the traditional method of bud-suction ramet propagation, tissue-cultured seedlings have the characteristics of stable genetic traits, uniform growth, rapid reproduction, and strong disease resistance. Therefore, tissue-cultured seedlings are often used in banana production. The number of root coefficients of tissue cultured seedlings and the fullness of fibrous roots are important standards...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N1/14C12R1/645
CPCA01H4/00A01H4/001C12N1/14
Inventor 程春振李丹齐全赖钟雄孙雪丽林玉玲郝向阳
Owner FUJIAN AGRI & FORESTRY UNIV
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