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Tissue culture and rapid propagation method for sspindus mukurossi

A technology for tissue culture, fast propagation and Sapindus japonica, applied in the field of plant tissue culture, can solve problems such as lack of supply of fine seedlings, restrictions on natural renewal and industrial application of Sapindus japonica, and lag in breeding of fine seeds, and reduce the natural environment. The degree of dependence, the effect of keeping the excellent characteristics unchanged, and reducing the production cost

Inactive Publication Date: 2015-06-03
黎有辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sapindus is a deciduous tree, which mainly reproduces by seed sowing. Its seed coat is bony and hard, and it is a hard seed. Under natural conditions, the rate of moths is high, the germination rate is very low, and the emergence is irregular, and the emergence period is long, which is seriously limited. Natural renewal and industrial application of Sapindus chinensis
At present, artificially planted Sapindus mostly use seedlings from natural provenance, but the basis for screening and breeding of improved varieties is weak, resulting in relatively lagging behind in breeding of improved varieties, lack of supply of excellent seedlings of improved varieties, and it is difficult to ensure continuous high yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: Sampling should be carried out on sunny days for more than 3 consecutive days and when there is no dew on the branches. The sampling time is between 9 and 10 in the morning. Take the young stems with axillary buds on the branches of Sapindus sativa in the same year as explants, wash off the dirt on the surface, soak in 3% washing powder dilution solution for more than 30 minutes, and then rinse under running water for more than 2 hours, turn to Go to the ultra-clean workbench for sterilization. Soak in 70% ethanol solution for 60s, rinse with sterile water for 5 times, then disinfect with 0.1% mercuric solution for 8 minutes, and then rinse the explants with sterile water until there is no foam. Finally, place the sterilized scales on sterile filter paper to blot the surface moisture for later use.

[0019] (2) Adventitious bud induction: Cut the stem segment with axillary buds about 2cm long in step (1) and inoculate it into the induc...

Embodiment 2

[0024] (1) Disinfection of explants: Sampling should be carried out on sunny days for more than 3 consecutive days and when there is no dew on the branches. The sampling time is between 9 and 10 in the morning. Take the young stems with axillary buds on the branches of Sapindus sativa in the same year as explants, wash off the dirt on the surface, soak in 3% washing powder dilution solution for more than 30 minutes, and then rinse under running water for more than 2 hours, turn to Go to the ultra-clean workbench for sterilization. Soak in 75% ethanol solution for 30s, rinse with sterile water for 5 times, then disinfect with 0.15% mercuric solution for 5 minutes, and then rinse the explants with sterile water until there is no foam. Finally, place the sterilized scales on sterile filter paper to blot the surface moisture for later use.

[0025] (2) Adventitious bud induction: Cut the stem segment with axillary buds about 2cm long in step (1) and inoculate it into the indu...

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Abstract

The invention discloses a tissue culture and rapid propagation method for sspindus mukurossi. The sspindus mukurossi is also named as soapbark, is a deciduous tree, is mainly propagated by seed sowing, and is hard in seed coat, high in moth rate, irregular in seedling emergence and long in growth period. Defects of seed propagation can be made up by tissue culture, the excellent characteristics of a mother plant are kept unchanged, the propagation speed is high, the propagation coefficient is high, the production cost can be reduced, and the needs of large-scale planting of the sspindus mukurossi are met. A stem with nodes of the sspindus mukurossi is taken as an explant, a sspindus mukurossi in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a sspindus mukurossi tissue culture and rapid propagation system is established, an effective way is provided for the large-scale seedling growing of the sspindus mukurossi, and foundation is laid for the factorial production and application of sspindus mukurossi nursery-grown plants.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Sapindus sapindus. Background technique [0002] Soapzi ( Sspindus mukurossi ), also known as soap tree, is a tall deciduous tree of Sapindaceae and is an important source of natural soap. It is mainly distributed in the south of the Huaihe River Basin and Guangdong, Fujian, Taiwan, Guangxi, Yunnan and other places, with a wide distribution area. Sapindus has a tall tree shape, straight trunk, dense crown, broad branches and leaves, strange leaf shape, and graceful posture. In autumn, the leaves are full of golden yellow, and the orange-yellow fruits are all over the branches. The viewing effect is excellent. Planting Sapindus as a greening plant on urban roads is also a very beautiful urban landscape. Large-scale cultivation of Sapindus can effectively green and control barren hills, and it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黎有辉
Owner 黎有辉
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