The invention relates to the technical field of fresh-keeping solutions, and discloses a preparation process of a fresh-keeping solution with a good fresh-keeping effect, the fresh-keeping solution comprises the following raw materials by weight: 0.1-1.5 parts of Chinese mugwort leaf, 0.5-2 parts of perilla stem, 0.3-2 parts of medical stone, 0.2-3 parts of fatty acid, 0.5-2.5 parts of chlorogenic acid, 39-61.4 parts of superoxide potential water, and 37-50 parts of purified water. According to the preparation process of the fresh-keeping liquid with the good fresh-keeping effect, Chinese mugwort leaves, perilla stems, medical stone and superoxide potential water are adopted as main raw materials, and the materials are common in the market, convenient to purchase and low in purchase cost; chinese mugwort leaves, perilla stems, medical stone and superoxide potential water are taken as main raw materials of the fresh-keeping liquid, so that escherichia coli, salmonella, staphylococcus aureus, vibrio parahaemolyticus and the like can be quickly killed, the generation and reproduction of putrefying bacteria can be effectively inhibited, bacteria and mildew are resisted, deterioration and rot are prevented, and the fresh-keeping storage time is effectively prolonged; and the components of the fresh-keeping solution are edible components, so that the fresh-keeping liquid is safe and non-toxic, and environmental pollution caused by the chemical fresh-keeping agent is avoided.