Method fermenting fungal hyphae to highly yield fungal polysaccharides
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A technology of fungal polysaccharide and mycelium, which is applied to the field of high-yield fungal polysaccharide fermented by fungal mycelium, can solve the problems of low yield and high cost of fungal polysaccharide, and achieve the effects of high-yield exopolysaccharide, shortening fermentation period and improving utilization rate.
Inactive Publication Date: 2018-09-04
NINGDE NORMAL UNIV
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[0004] The purpose of the present invention is to provide a method for high-yield fungal polysaccharide fermentati
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specific Embodiment approach 1
[0019] Specific embodiment one: what this embodiment mode records is a kind of method for fungal hyphae fermentation high-yield fungal polysaccharide, described method steps are as follows:
[0020] Step 1: Purchasing bacterial strains from Xinghua Fungi Research Institute, Gutian County, Fujian Province;
[0021] Step 2: Prepare slant preservation medium: take 200 g peeled potatoes and cut them into small pieces, add 800 mL of distilled water, boil for 20-30 min, filter with gauze to obtain the filtrate, add 20 g of glucose, 1 g of peptone, and 1 g of KH to the filtrate 2 PO 4 , 0.5g MgSO 4 ·7H 2 O. 20g of agar, heat to dissolve, add water to a total volume of 1L, keep the pH natural, and sterilize at 115°C for 20-30 minutes;
[0022] Step 3: Activating the strain: Inoculate the strain in Step 1 on the slant preservation medium in Step 2, culture at 22.5-25.5°C for 4-8 days, and obtain mycelium after culture;
[0023] Step 4: Preparation and cultivation of liquid seed med...
specific Embodiment approach 2
[0028] Specific embodiment two: the method for a kind of fungal hyphae fermentation high-yield fungal polysaccharide described in specific embodiment one, in step one, described bacterial strain is mushroom 881, Pleurotus eryngii 90, Tea tree mushroom No. 2, Flammulina velutipes 991 or bamboo Sun D02.
specific Embodiment approach 3
[0029] Embodiment 3: The method for fermenting high-yield fungal polysaccharides by fungal hyphae described in Embodiment 1, in step 5, the plant used in the plant extract is a plant of the genus Runnan.
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Abstract
A method fermenting fungal hyphae to highly yield fungal polysaccharides belongs to the technical field of microbial fermentation. The method comprises the following steps: purchasing the following strains: shiitake 881, Pleurotus eryngii 90, Agrocybe cylindracea No.2, shiitake 991 or Dictyophora indusiata D02; culturing a slant preservation medium at 22.5-23.5 DEG C for 4-8 d, and sterilizing a liquid seed medium; performing vibration cleaning on mycelia on the slant preservation medium with 10 mL of normal saline, inoculating a bacterium suspension accounting for 3-8% of the volume of the liquid seed medium, and performing shocking bed culturing under a rotating rate of 170-200 r/min at 23-26 DEG C; and sterilizing a liquid fermentation medium at 115 DEG C, controlling the volume of a liquid in a 500 mL triangular flask to be 100 mL, inoculating with the above obtained seed liquid accounting for 5-8% of the volume of the liquid fermentation medium, uniformly shaking the triangular flask, performing standing culturing for 4-8 h, and performing fermentation on a shaking bed for 4-6 h, wherein the culturing temperature is 23-26 DEG C, and the stirring rotating speed is 180-220 r/min. The fermentation conditions of the fungal polysaccharides are optimized, and water extract accounting for 10-50% of the amount of dried Lauraceae plant powder is added to promote the propagation ofthe fungal mycelia in the fermentation solution, so the biomass amount is increased by 68.45-114.36%, and the yield of extracellular polysaccharides is increased by 68.92-97.91%.
Description
technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for high-yield fungal polysaccharide fermentation by fungal hyphae. Background technique [0002] Fungal polysaccharides are a class of polysaccharides with high immunological activity, which widely exist in the fruiting body and mycelia of fungi. High-purity fungal polysaccharides can be used directly for oral administration or processed into injection preparations. Fungal polysaccharides can effectively improve the body's immunity and have less toxic and side effects. In the extraction of fungal polysaccharides, hot water extraction and solvent extraction are mainly used at home and abroad. Hot water extraction is a conventional extraction method, which has the advantages of mild extraction conditions, simple and convenient operation; but the disadvantage is that the yield of polysaccharides is not high. This field generally still adopts th...
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