Stable fungal fluorescent staining solution

Abstract

The present invention discloses a fungal fluorescent staining solution which is simple in operation, good in staining effect, high in detection rate, stable in system and easy to preserve. Fluoresceinin the fungal fluorescent staining solution can bind with beta-polysaccharides on the fungal cell wall with high affinity such as chitin and cellulose so that fungal components in a sample can be labeled and fungal morphology can be clearly observed under a fluorescence microscope. With the addition of an anti-interference fluorescent auxiliary in the staining solution, the binding rate of the fluorescent staining solution with the fungal cell wall is improved; moreover, the fluorescence intensity is enhanced under the ultraviolet excitation so that the propagules such as fungal hyphae and spores emit bright blue-white fluorescence; under the microscope observation, the outline of the fungi is clearly visible while the stability of the staining solution system is improved; and the fluorescence quenching risk of the staining solution is reduced. The staining solution is capable of one-step staining; staining can be completed in a few seconds; the operation is simple; the detection timeis saved; and the detection rate of the fungi is also improved.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and in particular relates to a fungal fluorescence detection dye. [0002] technical background [0003] Fungi belong to eukaryotes, without plastids, and their nutritional mode is absorption without phagocytosis. They can cause human and animal diseases, called mycoses; they can also cause plant diseases, human allergic diseases and mycotoxin poisoning. About tens of thousands of fungi have been found, of which more than 270 are pathogenic to humans. Fungal invasion of the human body is often secondary to other diseases, such as chronic wasting diseases such as malignant tumors and diabetes; large doses of X-ray radiation, immunosuppressants, etc. lead to fungal infection in patients with low immune function and resistance; patients who use a large number of adrenal cortex hormones , its inflammatory response is mostly suppressed, and it is also prone to fungal infection; in addition, ...

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Problems solved by technology

But its disadvantages are: 1. The operation process needs to strictly control the time and temperature, etc., and a slight mistake will affect the filming effect and the result judgment, and requires relatively high professional skills of the operator; 2. The operation is cumbersome and takes a long time (1 hour) 2-4 weeks for the culture method, which may lead to untimely diagnosis and delayed treatment; 3. Various reagents are required for each staining method, and management and storage are inconvenient

[0010] However, the fungal fluorescent staining solution has problems such as poor dyeing effect, fast quenching of the fluorescence effect after staining, and unstable storage of the staining solution for a long time, resulting in low fluorescence intensity after staining.

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