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Preparation method of metarhizium anisopliae conidia microcapsule emulsion

A technology for conidia and Metarhizium anisopliae, applied in the field of Metarhizium anisopliae, can solve the problems of atrophy of Metarhizium anisopliae, complex preparation process of Metarhizium anisopliae, avoid shrinkage, reduce culture time and use space, and achieve high reliability. Effect

Pending Publication Date: 2020-11-17
琼台师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation process of Metarhizium anisopliae is complicated, and due to the lack of protection in the preparation, Metarhizium anisopliae shrinks and dies, which needs further improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for the preparation of the Metarhizium anisopliae conidia microcapsule emulsion in the present embodiment comprises the following steps:

[0024] S1. Disinfection. First, disinfect the culture container, spray disinfectant, place it in a high-temperature box for high-temperature disinfection, and use an ultrasonic cleaning box to clean it. After cleaning and disinfection, the container is dried and set aside;

[0025] S2, preliminary cultivation, the container after disinfection is selected according to the capacity, after the selected capacity, Metarhizium anisopliae is placed into the container, and the fission between the mycelium septum is added in the container so that blastospores are produced in the container, and the cultivation time is 20h;

[0026] S3, medium-step culture, the conidia of Metarhizium anisopliae begin to germinate, the protoplasts are transferred to the growth point of the germ tube, the germ tube protrudes from one or both ends of the...

Embodiment 2

[0038] The method for the preparation of the Metarhizium anisopliae conidia microcapsule emulsion in the present embodiment comprises the following steps:

[0039] S1. Disinfection. First, disinfect the culture container, spray disinfectant, place it in a high-temperature box for high-temperature disinfection, and use an ultrasonic cleaning box to clean it. After cleaning and disinfection, the container is dried and set aside;

[0040] S2, preliminary cultivation, the container after disinfection is selected according to the capacity, after the selected capacity, Metarhizium anisopliae is placed into the container, and the fission between the mycelium and septum is added in the container so that blastospores are produced in the container, and the incubation time is 15h;

[0041] S3, medium-step culture, the conidia of Metarhizium anisopliae begin to germinate, the protoplasts are transferred to the growth point of the germ tube, the germ tube protrudes from one or both ends of ...

Embodiment 3

[0053]The method for the preparation of the Metarhizium anisopliae conidia microcapsule emulsion in the present embodiment comprises the following steps:

[0054] S1. Disinfection. First, disinfect the culture container, spray disinfectant, place it in a high-temperature box for high-temperature disinfection, and use an ultrasonic cleaning box to clean it. After cleaning and disinfection, the container is dried and set aside;

[0055] S2, preliminary cultivation, the container after disinfection is selected according to the capacity, after the selected capacity, Metarhizium anisopliae is placed into the container, and the fission between the mycelium septum is added in the container so that blastospores are produced in the container, and the cultivation time is 20h;

[0056] S3, medium-step culture, the conidia of Metarhizium anisopliae begin to germinate, the protoplasts are transferred to the growth point of the germ tube, the germ tube protrudes from one or both ends of the ...

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Abstract

The invention discloses a preparation method of a metarhizium anisopliae conidia microcapsule emulsion. The preparation method comprises the following steps of disinfecting, specifically, disinfectinga culture container firstly, spraying disinfectant fluid, putting in a high-temperature box for high-temperature disinfection, cleaning by using an ultrasonic cleaning box, and drying a container forlater use after cleaning and disinfection; preliminary culture, specifically, selecting the disinfected container according to the volume, placing the metarhizium anisopliae into the container afterselecting the volume, adding hypha diaphragms into the container, carrying out fission so as to generate spore buds in the container, and carrying out culture for 20 hours; and carrying out medium-step culture, specifically, enabling the metarhizium anisopliae conidia to begin to germinate, and transferring protoplast to a bud tube growth poin. The fungus conidium production speed is increased, the fungus culture time and the fungus use space are reduced, the safety and reliability during preparation are improved through multiple reasonable disinfection in the liquid culture stage, the phenomena of atrophy, death and the like of the metarhizium anisopliae are avoided, the reliability is high, and wide popularization can be achieved.

Description

technical field [0001] The invention belongs to the technical field of Metarhizium anisopliae, in particular to a method for preparing a Metarhizium anisopliae conidia microcapsule emulsion. Background technique [0002] Metarhizium anisopliae is very similar to the genus Penicillium in terms of taxonomic status. It develops well on the potato medium and forms white colonies first. After 2-3 days, the mycelial branches interweave and form conidiophores, and the spores grow green Clusters of fungal conidia, conidia are white at first, and then turn green or dark green when they become hot. The size of conidia varies greatly. Metarhizium anisopliae is a broad-spectrum insect pathogen. According to statistics, Metarhizium anisopliae hosts more than 200 species of insects, and can parasitize beetle beetles, weevils, golden needles, Lepidoptera pest larvae and hemiptera. Stinkbugs, etc. Insects can be induced to produce Metarhizium, which can form repeated infestation within th...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
CPCC12N1/14C12N3/00
Inventor 许天委王伟丁兆建郝慧华
Owner 琼台师范学院
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