31 results about "Phosphorylethanolamine" patented technology

The invention discloses a mesenchymal stem cell serum-free culture medium. The culture medium takes an L-DMEM, DMEM-F12 or alpha-MEM culture medium as a basic culture medium and also includes the following addition components: fibronectin, laminin, transferrin, trypsin, aprotinin, growth hormone, insulin, hydrocortisone, dexamethasone, bFGF, EGF, B27, IGF-I, IGF-II, choline, linoleic acid, sodium selenite, phosphorylethanolamine, glutathione, vitamin C, vitamin E, vitamin B12 and biotin. The culture medium has the advantages of defined composition and controllable quality, and mesenchymal stem cells cultured by the culture medium grow normal; and after multiple passages, the 'dryness' of the mesenchymal stem cells and the ability of the mesenchymal stem cells to be differentiated into osteoblasts, chondrocytes, adipocytes, neurons and other types of cells are still kept.

Particles formed from an alkai metal or alkaline earth metal and halide, for example, sodium and chloride, are provided. The particles can have a hydrophilic coating or external layer, formed of, for example, a polyether-lipid conjugate. In preferred embodiments, the lipid is a phospholipid, such as a phosphoethanolamine, and the polyether is a polyethylene glycol such as a PEG amine. Methods making the particles by, for example, a microemulsion reaction, are also provided. Pharmaceutical compositions including a plurality of particles and a pharmaceutically acceptable carrier are also disclosed. Typically the compositions include an effective amount of particles to treat a disease or condition, particularly cancer, in a subject in need thereof. The particles are typically nanoparticles, for example, between about 10 nm and 250 nm and can be monodisperse.

The invention discloses a preparation method of a turquoise blue water-soluble dye. The preparation method comprises the following steps of carrying out sulfonation reaction, chlorination reaction and after-treatment on chlorosulfonic acid and copper phthalocyanine which are taken as raw materials to obtain turquoise blue base filter cakes; adding water into the turquoise blue base filter cakes, pulping, adjusting the pH to be 5-5.5, adding phosphorylethanolamine, and reacting until the pH is 2.5-3 to obtain a reaction liquid I; adding a para-ester solution into the reaction liquid I and adding disodium hydrogen phosphate to control the pH to 6-7 to obtain a reaction liquid II after disodium hydrogen phosphate is completely consumed; decreasing the pH of the reaction liquid II to 5.5, adding N,N-dimethylformamide, adjusting the temperature to 25-35 DEG C, maintaining the pH to 5.5-6 with an aqueous solution of M2CO3 and reacting until the pH is changed by 0.1 to obtain a reaction solution III; and finally adding dispersants NNO and MF, standardizing and carrying out after-treatment to obtain the turquoise blue water-soluble dye.

The invention discloses a preparation method of a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs, comprising the following steps: step 1) preparation of NK cell membrane: adding NK cells to cells containing protease inhibitors In the lysis buffer, through ultrasonic centrifugation, NK cell membrane is obtained; Step 2) 1,2-distearoyl-sn-glyceryl-3-phosphoethanolamine-N-[amino (polyethylene glycol) 2000] ( Ammonium salt) and drugs are added into pure water, ultrasonicated to obtain liposome nanoparticles; step 3) NK cell membrane and liposome nanoparticles are co-extruded through a polycarbonate membrane with a pore size of 220 nm to form NK cell biomimetic nanocarriers. The NK cell biomimetic nanocarrier prepared by the invention is a delivery system for crossing the blood-brain barrier and specifically targeting glioma therapeutic drugs.

The invention discloses biomarkers in osteopeptide intervention treatment of osteoporosis, including lipids and lipid molecules, organic acids and their derivatives and / or neurotransmitter substances, and lipids and lipid molecules are the following substances Any one or several: taurine, arachidonic acid, 1‑palmitoyl‑2‑hydroxy‑sn‑glycerol‑3‑phosphoethanolamine, (4Z,7Z,10Z,13Z,16Z,19Z)‑4, 7,10,13,16,19‑docosahexaenoic acid, 1‑stearyl‑2‑hydroxy‑sn‑glycero‑3‑phosphocholine, taurodeoxycholic acid, taurodeoxychole acid and taurocholate. The invention discloses a screening method for osteopeptide anti-osteoporosis biomarkers. The invention discloses the use of biomarkers. The invention provides a demonstration study for the evaluation of the activity and function of natural products, and provides theoretical support for the systematic evaluation of the anti-osteoporosis activity of the bone peptide and the development of the bone peptide product with biological activity.

The invention belongs to the technical field of nanomaterials, and discloses a hydroxyapatite nanorod with surface grafted EGCG and a preparation method and application thereof. Firstly, oleic acid and octadecylamine were used as templates to synthesize monodisperse hydroxyapatite with controllable size, and its surface was aminated with phosphoethanolamine, and EGCG was grafted with the amino groups on the surface through amide bonds. The hydroxyapatite-grafted EGCG nanomaterials prepared by this method can induce osteosarcoma cell apoptosis, and have the potential to repair bone defects and support bone growth.

The invention relates to a method for preparing a phosphorylethanolamine compound, which belongs to the technical field of biochemistry. The method comprises the following steps of: adding ethanolamine or ethanolamine solution into one of phosphorus pentoxide, the solution of phosphoric acid, phosphorus oxychloride or the solution of phosphorus oxychloride at a certain reaction temperature to form a reaction mixture; heating the reaction mixture for vacuum-drying by using a direct program to form phosphorylethanolamine or phosphorylethanolamine hydrochloride; and dissolving the reaction product into water in a certain amount and then adding ethanolamine or the ethanolamine solution, wherein the mass ratio of the reaction product to the water in the certain amount is 1:1-100. As the water serves as a solvent, the method of the invention ensures high yield, vast raw material sources, low production cost, simple, safe and rational process, short production cycle and high purity of the prepared product, and avoids wastewater, waste gases and waste residues.

The invention discloses a serum-free culture medium for limbal stem cells and a culture method thereof. It contains basal medium and additional components; wherein, the additional components include: human recombinant EGF, insulin, 3,3', 5-triiodo-L-thyronine, hydrocortisone, forskolin element, manganese sulfate monohydrate, sodium selenite, sodium metasilicate, ammonium metavanadate, nickel chloride hexahydrate, stannous chloride dihydrate, ethanolamine, phosphoethanolamine, ammonium molybdate tetrahydrate, 4‑hydroxyethyl piperazine ethanesulfonic acid, vitamin C, bovine serum albumin, lipid concentrate and serum replacement. The serum-free medium of the present invention does not contain fetal bovine serum and any animal-derived components, can provide sufficient nutrition and a good environment for cell growth and proliferation, effectively replace the role of serum, and the cells grow well, and the purity and stability of the cells are improved. It provides a rapid and stable source of cells for research on the specific mechanism of limbal stem cells and transplantation therapy, and has broad prospects for clinical application.

The invention discloses an ultralight ultra-amphiphilic type three-dimensional graphene foam material, and a preparation method and application thereof. The preparation method comprises the steps of adopting graphene oxide and a green and environmental-friendly biochemical reagent phosphorylethanolamine as raw materials, firstly utilizing hydrothermal self-assembly for bonding phosphorylethanolamine molecules between graphene oxide layers, and then carrying out high-temperature annealing treatment on a graphene / phosphorylethanolamine compound to obtain the porous three-dimensional graphene foam material with a novel structure characteristic. The three-dimensional graphene foam material obtained through the invention has the characteristics of porosity and light weight, and has a higher infiltration capability on water / oil. Therefore, the graphene foam material provided by the invention can be used as an adsorbent or a catalyst so as to be applied in the fields such as marine oil leakage and water organic pollutant treatment, and meanwhile, the three-dimensional graphene foam material is stable in structure framework, and can be recycled repeatedly. The ultralight ultra-amphiphilic type three-dimensional graphene foam material provided by the invention is simple and practicable in preparation process, wide in raw material source, and favorable in preparation method repeatability, and can adapt to the requirement of industrial production.

The invention relates to a liquid fermentation culture medium for cordyceps sinensis. In additions to a carbon source, a nitrogen source, metal salts and the like in a common culture medium, the culture medium is further added with phosphorylcholine, phosphorylethanolamine, cholesterol, fatty acid and the like as a growth promoter, so that the cordyceps sinensis can be effectively promoted to form blastospore or hyphal bodies. Bacterial suspension obtained by culture medium fermentation is rich in blastospore or hyphal body growth point, and can be continuously fermented or used as a seed solution for subsequent liquid or solid fermentation, and therefore, the liquid fermentation culture medium is beneficial for improving fermentation production efficiency of the cordyceps sinensis.