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Application of juvenile hormone analogue methoxoprim in promoting stiffness of ghost-moth larvae

A technology of bat moth larvae and methoprene, applied in the promotion of ossified bat moth larvae, in the field of juvenile hormone analogue methoprene, which can solve time-consuming, bat moth ossification, artificial cultivation of Cordyceps sinensis, etc. problem, to achieve the effect of increasing the rigidity rate

Active Publication Date: 2022-02-01
力源新资源开发(广东)有限公司
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Problems solved by technology

[0005] Based on the above problems, the object of the present invention is to provide the application of the juvenile hormone analogue methoprene in promoting the ossification of bat moth larvae, thereby solving the time-consuming problem of injection infection of bat moth ossification and the long period of artificial cultivation of Cordyceps sinensis

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  • Application of juvenile hormone analogue methoxoprim in promoting stiffness of ghost-moth larvae

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Embodiment 1

[0016] Cordyceps sinensis strain Ophiocordyceps sinensis was isolated from wild Cordyceps sinensis in Kangding, Sichuan Province (code: KD) by tissue separation method, and stored at -80°C after molecular identification. Cordyceps sinensis broth (PMG): Potato 200g boiled juice, maltose 20g, peptone 10g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin B 1 0.02g, 5g grinding solution of fresh Mellonella mellonella larvae, mix and dilute to 1L with distilled water, dispense into 250mL Erlenmeyer flasks, 150mL per bottle, autoclave at 121°C for 30min, and use after cooling. On the ultra-clean workbench, the Cordyceps sinensis bacterium block (about 0.5cm 3 ) were inoculated in the above liquid medium, cultured on a shaker at 120 rpm at 9-13° C. for 45 days, and blastospores were collected. The method of collecting blastospores: filter the liquid culture solution with 3 layers of sterile lens-cleaning paper, collect the filtrate into a 50mL sterilized centrifug...

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Abstract

The invention discloses an application of a juvenile hormone analogue methoxoprim in promoting stiffness of ghost-moth larvae. In order to solve the problems of long artificial culture period, low efficiency and the like of Chinese caterpillar fungus caused by large time consumption in the stiffness process of injected and infected ghost-moth larvae, a juvenile hormone analogue methoxoprim is added to inject and infect the ghost-moth larvae together with cordyceps sinensis blastospores, then the stiffness rate (61.85 + / -3.37%) of theghost-moth larvae is increased, and the stiffness rate is obviously higher than that of larvae only injected with blastospores (48.15 + / -2.69%), so that the stiffness rate of the larvae can be obviously improved by simultaneously injecting the cordyceps sinensis blastospores and the methoxoprim.

Description

Technical field: [0001] The invention belongs to the technical field of artificial cultivation of Cordyceps sinensis, and in particular relates to the application of a juvenile hormone analogue methoprene in promoting the ossification of bat moth larvae. Background technique: [0002] Cordyceps sinensis is a complex of insects and fungi formed by Cordyceps sinensis Ophiocordyceps sinensis infecting bat moth larvae. It is a rare and precious biological resource unique to the Qinghai-Tibet Plateau. With the improvement of consumers' recognition of Cordyceps sinensis, the consumption has increased sharply, and the output of wild Cordyceps sinensis has been unable to meet market demand. After decades of research, major breakthroughs have been made in the artificial cultivation of Cordyceps sinensis, including the artificial cultivation of the fruiting bodies of the fungus Cordyceps sinensis, the large-scale artificial rearing of the host insect bat moth at low altitudes, and the...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01K67/033
CPCA01G18/00A01K67/033
Inventor 吴华曹莉熊佳福周雯韩日畴
Owner 力源新资源开发(广东)有限公司
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