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Isaria cateinannulata as well as product, preparation method and use method thereof

A coryneform spore and ring chain technology, applied in the field of coryneform spore and its products and preparation, can solve the problems of chlorosis and yellowing of leaves, accelerated plant death, reduced plant vigor, etc. Prospect of application promotion, low cost effect

Inactive Publication Date: 2015-09-23
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adult mites, juvenile mites, and nymphs feed on the juice of crops with piercing-sucking mouthparts, causing leaves to turn green and yellow and appear necrotic spots, petals wither like spray burns, and the vitality of the entire plant is reduced, which accelerates the death of the plant

Method used

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  • Isaria cateinannulata as well as product, preparation method and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Embodiment 1 of the present invention: I. Isaria cateniannulata Obtaining the 08XS-1 strain: Wash the pupae of Lepidoptera, inoculate it on the PDA medium according to the separation method of single mycelia, and purify it to obtain I. Isaria cateniannulata 08XS-1 strain, stored at 4°C for later use.

Embodiment 2

[0014] Embodiment 2 of the present invention: the preparation method of biopesticide, get Isyriamus spp. Isaria cateniannulata The 08XS-1 strain is cultivated in a liquid medium at 200 rpm and 27°C for 10 days, and then the bacterial suspension is filtered with double-layer lens-cleaning paper to obtain a spore suspension.

Embodiment 3

[0015] Example 3 of the present invention: the use of biological pesticides, during July 18-30, 2014, at the experimental base of Guizhou University, Huaxi, Guiyang City, Guizhou Province (1131m above sea level, 26°24' north latitude, 106°40' in Tokyo), The soil type is yellow soil, with good fertility, convenient drainage and irrigation, and consistent water and fertilizer management in each experimental plot; after the cucumber seedlings were raised, no pesticides were used except for watering and fertilizing. The experiment was repeated 3 times, with 15 plots for each vegetable, and the area of ​​each plot was about 15m 2 (About 15m long single ridge), one ridge blank (without any treatment) is set up as a protected area between the plots, a total of 45 plots; the leaves are sprayed, and the actual spore concentration of the spray liquid is 2 × 10 8 pcs / ml, about 1000ml of liquid sprayed per plot, with clear water as the control, the same amount of liquid sprayed for treatm...

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Abstract

The invention provides isaria cateinannulata as well as a product, a preparation method and a use method thereof. Blastospore suspension liquid of isaria cateinannulata 08XS-1 bacterial strain is adopted as effective ingredients to prepare biopesticide; the biopesticide can kill insects without adopting an oral administration manner or contacting the insects, as well as can effectively prevent and treat tetranychus urticae koch on vegetables in fields; the biopesticide has incomparable advantages than other biopesticide; the blastospore suspension liquid is taken as bioactive substances, so that the isaria cateinannulata is more available and high in toxicity; in addition, the isaria cateinannulata has no side effects on euseius nicholsi, so that the isaria cateinannulata has low toxicity on natural enemies; the isaria cateinannulata can be used for further development and production of biopesticide along with brighter application and popularization prospects; the method provided by the invention is simple, easy to implement, low in cost and good in using effect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Isymus annuli and its products, preparation methods and usage methods. Background technique [0002] Two-spotted spider mite ( Tetranychus urticae Koch 1863), also known as two-spotted spider mite, belongs to the genus Tetranychus genus Tetranychus. It is reported that the mite damages more than 1100 kinds of crops, including crops, horticultural crops, greenhouse vegetables and ornamental plants, etc. It has become a worldwide economic Harmful mites. Adult mites, juvenile mites, and nymphs feed on the juice of crops with piercing-sucking mouthparts, causing leaves to turn yellow and appear necrotic spots, petals withered like spray burns, and the vitality of the entire plant is reduced, which accelerates the death of the plant. Due to its short life cycle and alternation of generations, as well as the increased resistance to chemical insecticides over a long period of time, scien...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/02A01N25/04C12R1/645
CPCA01N63/30C12N1/14
Inventor 金道超张晓娜邹晓郭建军陈亭旭
Owner GUIZHOU UNIV
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