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Application of wood vinegar in promoting germination of Esteya vermicola bud spores to generate crescent spores

A technology of blastospores and wood vinegar, applied in the field of microorganisms, can solve the problems of short duration, slow effect, difficult to fully cover tree trunks, etc., and achieve the effects of improving work efficiency, preventing and controlling efficiency, and having great application value.

Pending Publication Date: 2022-05-10
中国林业科学研究院森林生态环境与自然保护研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Disposal of infested wood, trunk injection and control of vector insects are currently the main methods for the prevention and control of pine wood nematode disease. However, due to the difficulty of full coverage of tree trunk injection, the cost of physical control of vector insects is high and the duration is short, and the parasitism rate of natural enemies of vector insects is low. , the effect is slow, and the treatment effect of infested wood is relatively lagging behind. The use of microbial pesticides to control pine wood nematode disease is a current research hotspot.

Method used

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  • Application of wood vinegar in promoting germination of Esteya vermicola bud spores to generate crescent spores
  • Application of wood vinegar in promoting germination of Esteya vermicola bud spores to generate crescent spores
  • Application of wood vinegar in promoting germination of Esteya vermicola bud spores to generate crescent spores

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Effect test

Embodiment 1

[0037] The application of wood vinegar in promoting the germination of Esteya vermicola blastospores to produce crescent-shaped spores comprises the following steps:

[0038] (1) The Esteya vermicola FXY121 strain stored in the laboratory was transferred to a PDA medium plate for activation, and placed in a constant temperature incubator at 25° C. for 7 days in the dark.

[0039] (2) Use a sterile puncher on the ultra-clean workbench to punch 4 pieces of bacteria cakes with a diameter of 6mm from the edge of the PDA plate colony and inoculate them into the PDB liquid medium, and put them in an incubator at 25°C and 200r / min for cultivation 7d. After 7 days, the culture solution was filtered through three layers of sterile gauze to obtain a suspension of blastospores of Esteya vermicola. Adjust the concentration of spore suspension to 1*10 5 / mL spare.

[0040] (3) Filter the stock solution of wood vinegar solution (purchased in the market) with a 0.2 μm sterile microporous ...

Embodiment 2

[0044] The application of wood vinegar in promoting the germination of Esteya vermicola blastospores to produce crescent-shaped spores comprises the following steps:

[0045] (1) The Esteya vermicola Cxy1839 strain stored in the laboratory was transferred to a PDA medium plate for activation, and placed in a constant temperature incubator at 25° C. for 7 days in the dark.

[0046] (2) Use a sterile puncher on the ultra-clean workbench to punch 4 pieces of bacteria cakes with a diameter of 6mm from the edge of the PDA plate colony and inoculate them into the PDB liquid medium, and put them in an incubator at 25°C and 200r / min for cultivation 7d. After 7 days, the culture solution was filtered through three layers of sterile gauze to obtain a suspension of blastospores of Esteya vermicola. Adjust the concentration of spore suspension to 1*10 5 / mL spare.

[0047] (3) Filter the stock solution of wood vinegar solution (purchased in the market) with a 0.2 μm sterile microporous...

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Abstract

The invention is applicable to the technical field of microorganisms, and provides application of wood vinegar in promoting Esteya vermicola spores to germinate and produce crescent spores, the wood vinegar is applied in the germination stage of the Esteya vermicola spores, the number of the crescent spores produced by the Esteya vermicola spores can be remarkably increased, and particularly, the number of the crescent spores produced by the Esteya vermicola spores can be remarkably increased after the wood vinegar is added into a sterilized water agar culture medium and then the water agar culture medium is poured into the water agar culture medium, so that the yield of the crescent spores produced by the Esteya vermicola spores is increased. And after solidification, coating with a germinated spore suspension of Esteya vermicola, and culturing in a constant-temperature incubator at 25 DEG C for 5 days to obtain a large number of crescent spores. By adding the wood vinegar, Esteya vermicola bud spores can germinate to generate more crescent spores, the mechanism research of killing pine wood nematodes by the crescent spores can be conveniently carried out, the working efficiency of researchers is improved, and the wood vinegar has an induced resistance function, so that the wood vinegar has a good application prospect. And if being applied together with an Esteya vermicola bud spore suspension in the field, the compound has a synergistic effect on prevention and control of the pine wood nematode disease, and has a great application value in the future.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the application of wood vinegar in promoting the germination of Esteya vermicola blastospores to produce crescent-shaped spores. Background technique [0002] Pine wood nematode (Bursaphelenchus xylophilus) is one of the most dangerous and destructive pests in the global forest ecosystem. High-altitude areas such as the temperate zone, the middle temperate zone, and the Qinling Mountains have caused large areas of pine forest to die, causing huge economic and ecological losses, and the epidemic area is still increasing [0003] Disposal of infested wood, trunk injection and vector insect control are currently the main methods for the prevention and control of pine wood nematode disease. However, due to the difficulty of full coverage of tree trunk injection, the cost of physical control of vector insects is high and the duration is short, and the parasitism rat...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12R1/645
CPCC12N3/00C12N1/14
Inventor 理永霞温晓健王璇刘振凯张雷张星耀
Owner 中国林业科学研究院森林生态环境与自然保护研究所
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