Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of l-proline in increasing the number of blastospores and mycelial biomass of Cordyceps sinensis

A technology for Cordyceps sinensis fungus and blastospores, which is applied in the directions of application, plant cultivation, botanical equipment and methods, etc., can solve the problems such as the excessive excavation of Cordyceps sinensis has not been effectively curbed, shorten the artificial cultivation time, reduce the cultivation cost, and improve the The effect of efficiency

Active Publication Date: 2021-10-15
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Cordyceps sinensis has been listed as a second-class protected species in the List of National Key Protected Wild Plants, the overharvesting of Cordyceps sinensis has not been effectively curbed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of l-proline in increasing the number of blastospores and mycelial biomass of Cordyceps sinensis
  • Application of l-proline in increasing the number of blastospores and mycelial biomass of Cordyceps sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and add 1.5g of L-proline (final concentration is 10g / L, without L-proline as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0024] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at 12±2°...

Embodiment 2

[0026] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, and add 3g of L-proline (final concentration is 20g / L, without adding L-proline as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0027] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at ...

Embodiment 3

[0029] Preparation of Cordyceps sinensis liquid medium: 30g boiled potato filtrate (weigh 30g peeled potatoes, add appropriate amount of water to boil for 20min, filter, take the filtrate, which is 30g boiled potato filtrate), 1.5g peptone, 3g Maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a 500mL Erlenmeyer flask, add 0.38mg L-proline (final concentration is 2.5mg / L, no L-proline is used as a control), and dilute to 150mL with water. After autoclaving, place in a 12±2°C culture room to pre-cool for later use.

[0030] Inoculate the inoculation mother liquor into the 500mL Erlenmeyer flask that 150mL Cordyceps sinensis liquid culture medium is housed, insert 1mL inoculation mother liquor to the final concentration of each bottle of culture medium to be 10 7 conidia / mL, wherein each bottle of culture medium contains about 15 blastospores, place the flask on a shaker with a rotation speed of 100 rpm, and cultivate it at 12±2°...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of L-proline in increasing the number of blastospores and mycelia biomass of Cordyceps sinensis. The present invention aims at the problem of how to increase mycelium biomass in the liquid fermentation process of Cordyceps sinensis and how to obtain a large number of blastospores for infection in the process of artificial bionic culture of Cordyceps sinensis combined with Cordyceps sinensis, adding L-proline to the Cordyceps sinensis culture medium to induce Cordyceps sinensis Bacteria produced blastospores efficiently, and the number of blastospores in the medium added with L-proline increased by 1.49 to 5.26 times after being cultured with Cordyceps sinensis spores for 30 days, and the number of blastospores after 45 days of culture increased by 17 to 52.6 times, After culturing for 60 days, the dry weight of mycelium increased by 15.67-66.44%. The invention can more efficiently obtain blastospores for infecting bat moths for biomimetic culture of insect fungus combined with Cordyceps sinensis, can improve the efficiency of obtaining mycelium by liquid fermentation of Cordyceps sinensis fungus, shortens the time for artificial cultivation of Cordyceps sinensis, and reduces the cultivation cost.

Description

Technical field: [0001] The invention belongs to the technical field of fungal culture, and in particular relates to the application of L-proline in increasing the number of blastospores and mycelia biomass of Cordyceps sinensis. Background technique: [0002] Cordyceps sinensis Ophiocordyceps sinensis is a traditional precious Chinese herbal medicine in my country. Together with ginseng and antler, it is known as the three treasures of Chinese medicine. Cordyceps sinensis is a complex of dead insects and fungal sporosomes formed by infecting the larvae of the family Hepialidae by Cordyceps sinensis fungus (also known as Hepialidae sinensis). Cordyceps sinensis has a special habitat (it only reproduces naturally in the high-altitude alpine meadow environment), with slow growth rate, long cycle and extremely limited natural resources. Although Cordyceps sinensis has been listed as a second-class protected species in the "List of National Key Protected Wild Plants", the over-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 刘桂清曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com