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Application of n-acetylglucosamine in promoting the development of blastospores of Cordyceps sinensis and the formation of mycelium

A technology of acetylglucosamine and Cordyceps sinensis, which is applied to microorganism-based methods, fungi, microorganisms and other directions, can solve the problems of long time for developing and forming mycelium, low efficiency of artificial cultivation of Cordyceps sinensis, etc., and achieves shortened time, mycelium The effect of increasing the formation rate

Active Publication Date: 2021-08-13
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above problems, the object of the present invention is to provide the application of N-acetylglucosamine in promoting the development and formation of mycelia of the blastospores of Cordyceps sinensis, thereby solving the problems of long time for the development and formation of mycelia of the blastospores of Cordyceps sinensis and the low efficiency of artificial cultivation of Cordyceps sinensis

Method used

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  • Application of n-acetylglucosamine in promoting the development of blastospores of Cordyceps sinensis and the formation of mycelium
  • Application of n-acetylglucosamine in promoting the development of blastospores of Cordyceps sinensis and the formation of mycelium

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Effect test

Embodiment 1

[0021] Prepare Cordyceps sinensis liquid culture medium, with 30g boiled potato filtrate (30g potato is boiled with water, then filter, take filtrate, be 30g boiled potato filtrate, the same below), 1.5g peptone, 3g maltose, 0.08 g magnesium sulfate, 0.25 g potassium dihydrogen phosphate, 3 mg vitamin B 1 Put it into a conical flask, and dilute to 150ml with water. After autoclaving, place in a culture room at 12±2°C to pre-cool, insert blastospores into every 150mL liquid medium ( figure 1 ) to make the final concentration 10 5 each / mL, and add N-acetylglucosamine (without adding N-acetylglucosamine as a control) to make the final concentration 5mg / l, place it on a shaker with a rotation speed of 100 rpm, and store it at 12±2°C , cultivate under dark conditions, cultivate the 2nd day, take out 1mL culture bacterium liquid, microscopic examination finds that blastospore begins to germinate, cultivate the 8th day, take out 1mL culture bacterium liquid, the mycelia of blastosp...

Embodiment 2

[0023] Prepare Cordyceps sinensis liquid culture medium, 30g boiled potato filtrate, 1.5g peptone, 3g maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a conical flask, and dilute to 150ml with water. After autoclaving, place in a culture room at 12±2°C for pre-cooling, insert blastospores into every 150mL of liquid medium to make the final concentration of 10 7 each / mL, and add N-acetylglucosamine (without adding N-acetylglucosamine as a control) to make the final concentration 5mg / l, place it on a shaker with a rotation speed of 100 rpm, at 12°C, in the dark On the second day of cultivation, take out 1mL culture liquid, and microscopic examination reveals that blastospores begin to germinate. On the eighth day of cultivation, take out 1mL culture liquid, and the mycelia formation rate of blastospores reaches 54% under a microscope. The mycelium formation rate increased by 127.94% compared with the control without adding the ...

Embodiment 3

[0025] Prepare Cordyceps sinensis liquid culture medium, 30g boiled potato filtrate, 1.5g peptone, 3g maltose, 0.08g magnesium sulfate, 0.25g potassium dihydrogen phosphate, 3mg vitamin B 1 Put it into a conical flask, and dilute to 150ml with water. After autoclaving, place in a culture room at 12±2°C for pre-cooling, insert blastospores into every 150mL of liquid medium to make the final concentration of 10 5 each / mL, and add N-acetylglucosamine (without adding N-acetylglucosamine as a control) to make the final concentration 50mg / l, place it on a shaker with a rotation speed of 100 rpm, and store it at 12±2°C , cultivated under dark conditions, cultured on the second day, took out 1mL of the culture liquid, microscopic examination found that blastospores began to germinate, cultured on the eighth day, took out 1mL of culture liquid, and examined under a microscope that the hyphae formation rate of blastospores reached 51.11 %, the rate of mycelium formation was 107.17% hig...

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Abstract

The invention discloses the application of N-acetylglucosamine in promoting the development of spores of Cordyceps sinensis to form hyphae. The present invention aims at the problems such as the long time for the development and formation of mycelia of the blastospores of Cordyceps sinensis, the long period and low efficiency of the artificial culture of Cordyceps sinensis, and N-acetylglucosamine is added to the culture medium of Cordyceps sinensis to induce the mycelium of the blastospores, and the second culture It was found that the blastospores germinated to form hyphae within 1 day, and 62% of the blastospores germinated to form long hyphae on the 8th day of cultivation, and the mycelium formation rate was significantly increased compared with that without adding inducing substances. The invention promotes the growth of blastospores in the form of hyphae, and shortens the time for artificial cultivation of Cordyceps sinensis.

Description

Technical field: [0001] The invention belongs to the technical field of fungal culture, and in particular relates to the application of N-acetylglucosamine in promoting the development of blastospores of Cordyceps sinensis to form hyphae. Background technique: [0002] Cordyceps sinensis Ophiocordyceps sinensis is a traditional precious Chinese herbal medicine in my country. Together with ginseng and antler, it is known as the three treasures of Chinese medicine. Cordyceps sinensis is a complex of dead insects and fungal sporosomes formed by infecting the larvae of the family Hepialidae by Cordyceps sinensis fungus (also known as Hepialidae sinensis). Cordyceps sinensis has a special habitat (it only reproduces naturally in the high-altitude alpine meadow environment), with slow growth rate, long cycle and extremely limited natural resources. Although Cordyceps sinensis has been listed as a second-class protected species in the "List of National Key Protected Wild Plants", ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/14C12R1/645
Inventor 刘桂清曹莉韩日畴
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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