59 results about "Metarrhizium anisopliae" patented technology

The invention relates to the field of banana cultivation, in particular to a compound microbial agent for preventing and treating a banana wilt disease and a prevention and treatment method. The compound microbial agent comprises paenibacillus polymyxa, bacillus subtilis, bacillus thuringiensis, pseudomonas stutzeri, bacillus amyloliquefaciens, cold-resistant brevibacteriaceae, trichoderma and metarrhizium anisopliae. By means of a reasonable proportion, all strains are mutually influenced and promoted, and the prevention and treatment effect on the banana wilt disease is greatly improved. The prevention and treatment method of the banana wilt disease can effectively adjust the composition of bacterial colonies in soil, promote growth and propagation of probiotics and antagonistic bacteria in the soil, rapidly restore and stabilize the balance of the bacterial colonies in the soil, prevent and treat occurrence and spread of the banana wilt disease, effectively decrease the occurrence rate of the banana wilt disease and maintain the stable and high yield of bananas.

The invention provides a disinsection fungi emulsifiable powder, comprising the following components: disinsection fungi spore powder or mycelium 40-60, vegetable oil 5-15, surfactant 5-10, stabilizer 5-10, dispersant 0.1-1; wherein the disinsection fungi is metarrhizium anisopliae, white muscardine fungi, paecilomyces, hirsutella or the mixture; the surfactant is nonionic surfactant, the vegetable oil is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the palm oil, corn oil, soybean oil, cotton seed oil and colza oil; the stabilizer is one kind or the mixture of many kinds of the oil according to arbitrary ratios selected from the xanthan gum, carboxymethyl cellulose, gelatin; the dispersant is DW 1003, glucose ester of fatty acid or the mixture thereof. The storage and transportation of the disinsection fungi emulsifiable powder is convenient and the storage period of the fungi spore is effectively prolonged and the long-term storage is convenient and the preparation activity is higher; after application of the disinsection fungi emulsifiable powder, the oil can effectively absorb the ultraviolet ray and reduce the damage of the ultraviolet ray on the fungi spore, effectively increase the field control-effect stability. The process production technique of the disinsection fungi emulsifiable powder is simple and the disinsection fungi emulsifiable powder is easily mixed with the other compatible synergistic pesticide.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention belongs to the technical field of biology, and particularly relates to a biological pesticide for killing aphids and a preparation method for the biological pesticide. The biological pesticide comprises the main components of nontoxic and harmless cinnamomum camphora steam and leaf extract (camphor), plant ash leachate, beauveria bassiana, metarrhizium anisopliae, yeasts and glucose, so that insecticidal effects of the biological pesticide are greatly improved, and organic nutrients are provided for plants to promote the growth of the plants. The application of the biological pesticide is relatively specific, and the biological pesticide is mainly used for damage caused by aphids to various plants, and also has unobvious prevention and control effects on other insect damage. The biological pesticide has the characteristics of nontoxicity, high efficiency, no contamination, no pollution and no residues, is an important means for realizing green ecology, and has broad development prospect.

The invention discloses entomogenous fungi chafer metarrhizium anisopliae strain CQMa102 acidity trehalase and its manufacturing method. It adopts active chafer metarrhizium anisopliae strain CQMa102 spore to form enzyme in culture medium and purify it. The formed protease is glycoprotein. Its isoelectric point is 4.7. Molecular weight is 170kDa. Trehazolin is its specific inhibition. And its IC50 is 1.3*10-8M. The protein plays important role in trehalose competive in infection insect course. Meanwhile the purified trehalase can be applied to its content qualitative and quantitative detection in industry and other fields.

The invention relates to a biological pesticide composition, in particular to a fungus and plant extract pesticide composition. An insecticidal composition containing metarrhizium anisopliae and veratrine is characterized by mainly comprising the metarrhizium anisopliae and the veratrine, wherein the metarrhizium anisopliae and the veratrine are mixed according to the weight ratio being (1-20):(10-2). The metarrhizium anisopliae is metarrhizium anisopliae conidia, and the content is 5-40 billion spores / gram. The veratrine is a pure substance or a mixture containing veratrum total alkali. The insecticidal composition is environmentally friendly, and can expand the insecticidal spectrum of biological pesticides, be higher in insecticidal effect and longer in lasting period, and reduce the use frequency of pesticides.

The invention relates to the field of biological pesticides, and specifically relates to a metarrhizium anisopliae granular preparation and a preparation method thereof. The metarrhizium anisopliae granular preparation comprises the following components in percentage by weight: 1-5% of metarrhizium anisopliae conidial powder, 90-95% of carriers, 0.01-0.45% of a promoting agent, 0.01-0.45% of a protective agent, and 1-5% of a lubricant. According to the technical scheme of the invention, the addition of aids is reduced, thereby significantly reducing the processing and manufacturing costs of granules. Under the premise of not adding an adhesive, a disintegrating agent and a disintegrating aid, through using the self physical and chemical properties of the carriers and the adjustment of water addition, the granulating property and the disintegration property of the carrier are solved well. Granules are relatively easy to dry, thereby reducing the energy consumption of drying. Through the adjustment of water addition, heat generated by granulating can be greatly reduced, and the damages on metarrhizium anisopliae spores caused by the granulating process can be reduced. Through adding small amount of the promoting agent, the germination of metarhizium anisopliae spores can be significantly promoted; and the damages on spores caused by the granulating process can be significantly reduced, and the problems of thermal damages and mechanical damages in the granulating process are solved better.

A process for preparing a non-woven disinfecting strip of Metarrhizium anisopliae includes sampling the bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating the Metarrhizium anisopliae to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.

The invention discloses an environment-friendly pesticide for forestry, which is characterized by being composed of the following components in parts by weight: 1-3 parts of Metarrhizium anisopliae powder, 2-4 parts of Hirsutella thompsonii powder, 0.3-0.5 part of aschersonia powder, 1-3 parts of bacillus thuringiensis var. powder, 3-6 parts of laminaria, 1-2 parts of rhizoma anemarrhenae, 0.1-0.2 part of frankincense, 1-4 parts of eupatorium, 2-8 parts of cibot rhizome, 3-6 parts of Herba Lycopi, 4-7 parts of rhizoma alismatis, 2-4 parts of rhodococcus powder, 23-34 parts of arum, 11-19 parts of cowbane, 1-16 parts of elegant jessamine, 2-3 parts of platycarya strobilacea powder, 5-18 parts of creat and 100 parts of water. The green pesticide for trees and flowers is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free.

The invention discloses a pesticidal main agent by compounding destruxins of Metarrhizium anisopliae and decamethrin. The main agent is a mixture of the destruxins of Metarrhizium anisopliae and the decamethrin; and the mass ratio of the destruxins of Metarrhizium anisopliae to the decamethrin is 1-9:9-1. The pesticidal main agent selects the the destruxins of Metarrhizium anisopliae and the decamethrin for compounding, and determines the optimal mixing ratio of both, so as to obtain obvious synergy and effectively solve the drugfastness problem of pests such as diamond back moth and prodenia litura. The pesticidal main agent has good desinsection effect and strong practical application and promotion, and has important significance of preventing and controlling the pests such as the diamond back moth and the prodenia litura; and the pesticide is environment-friendly.

The invention discloses a pesticide compounding beauveria bassiana fungus and destruxin. The pesticide is prepared by mixing the beauveria bassiana fungus, the destruxin and solvent, the concentration of live conidia of the beauveria bassiana fungus after mixing ranges from 10<7> to 10<11> conidia per liter, and the pesticidal effect is better when the concentration of the destruxin ranges from 10 mg / L to 100 mg / L. The preparation method for the pesticide comprises the following steps: preparing conidial suspension of the beauveria bassiana fungus, preparing destruxin stock solution, mixing the conidial suspension and the destruxin stock solution, and the like. The invention selects the beauveria bassiana fungus to be used with the destruxin in a mixing way for synergic action, and the pesticide has the completely new function mechanism different form the prior pesticide, has an excellent pesticidal effect particularly in poisoning and killing bemisia tabaci gennadius with serious harm, and meanwhile is environment-friendly.

The invention discloses a separating and purifying method of Metarrhizium anisopliae, which comprises the following steps: (1) adsorbing fungal ferment liquid to remove partial impurity; removing adsorbent through centrifuging; obtaining supernatant layer of ferment liquid; (2) extracting supernatant layer under ultrasound; separating organic phase and water phase through separatory funnel; reserving organic phase to condense into rough extract of Metarrhizium anisopliae; (3) decompressing rough extract of Metarrhizium anisopliae; condensing and drying fraction; dissolving and filtering; (4) making spectrum; separating and purifying the rough product in the high-effective liquid phased chromatograph to produce high-purity product.

The invention relates to a Metarhizium anisopliae var.argyrogramma agnata GXW26-8 (CCTCC No:M204078) for eradicating multiple agronomic crop pests, and also relates to the method for cultivation and use in disinsection. The MaGXW26-8 can be used for developing biological insecticide.

The invention relates to a solid culture method of metarrhizium anisopliae and the technical field of biological fermentation method. The method comprises preparation of liquid strain and solid culture. The prior period of the solid culture uses framework culture and the later period uses plastic bag culture. The solid culture medium includes nutritious raw material wheat bran and maize strain power as a raw material carrier. The beneficial effects of invention are that the combination of framework culture and plastic bag culture can effectively reduce pollution probability in the culture process and the requirements on humidity in the later period and obviously improve the ventilation performance of the culture medium; the fermentation technology is simple and does not need complex equipment, the fermentation period is short, the production efficiency is high, the production cost is low, and the economical benefit and social benefit are relatively high.

The invention relates to biopesticide for preventing cydia pomonella. The biopesticide is harmless to people and livestock, generates no phytotoxicity on crops and is free of pesticide residue and environment pollution. The biopesticide comprises following extracts, auxiliaries and synergists including, wherein the extracts include, by weight, 150-200 parts of matrine, 100-150 parts of nicotine, 150-200 parts of cynanchum komarovii, 50-70 parts of tripterygium wilfordii, 80-120 parts of saponin, 50-80 parts of agrimonia pilosa, 40-60 parts of stellera chamaejasme, 80-120 parts of melia azedarach fruit, 40-60 parts of melia azedarach bark, 80-100 parts of pyrethrum flower, 50-80 parts of radix stemonae, 50-80 parts of geranium wilfordii, 200-500 parts of euphorbic acid; the auxiliaries are penetrant, emulgator and stabilizer; the synergists are fungal biocontrol agents composed of trichoderma, penicillium, chaetomium, cylindrocarpon tonkinense, fusarium, beauveria bassiana and metarrhizium anisopliae.

The invention discloses a green microbial biofertilizer which is characterized by comprising the following components in parts by weight: 0.1-0.2 part of ferment bacteria, 0.1-0.2 part of bacteriophage powder, 0.1-0.2 part of Oceanospirillales powder, 0.1-0.2 part of beauveria bassiana powder, 0.1-0.2 part of Metarrhizium anisopliae powder, 3-7 parts of yam, 1-3 parts of sucrose, 1-7 parts of agar, 70-95 parts of corn straw, 55-75 parts of bean straw, 25-35 parts of potato straw, 1-8 parts of ground phosphorite and 2-7 parts of oyster shell. The organic fertilizer disclosed by the invention can implement plant growth, promote plant growth and inhibit various pathogenic bacteria in soil from growth by enhancing the autoimmune function of plants, promoting plant growth and improving soil.

The invention relates to the field of plant protection, in particular to specific primers for detecting endogenousness of metarrhizium anisopliae in plant roots. The sequences of the specific primers are F1:5'-GGGGTAGTCAGTATTCTGGCGGGC and R1:5'-GGGGTTCCACGGCGAGACCGCCAAT. The detection sensitivity reaches 1:2500.

The invention discloses a pesticidal main agent and a pesticide by compounding destruxins of Metarrhizium anisopliae and high-efficiency cypermethrin. The mass ratio of the destruxins of Metarrhizium anisopliae to the high-efficiency cypermethrin in the main agent is 7-1:3-9. The invention adopts pyrethroid pesticide with high efficiency, low toxicity, low residue and broad spectrum activity, namely the high-efficiency cypermethrin, and entomopathogenic fungi with obvious disinsection effect, namely the destruxins of Metarrhizium anisopliae, for compounding to obtain the pesticidal main agent, and determines the preferable compounding ratio of both through a large number of experiments. The pesticide has brand-new action mechanism different from the prior pesticides and obvious disinsection effect, particularly has obvious toxic action on prodenia litura which has severe harm, and has the characteristic of environmental protection at the same time.

The invention relates to an insecticide compound with the complementary action and the synergetic effect. The insecticide compound comprises the active ingredients of guadipyr and metarrhizium anisopliae, wherein the weight ratio of guadipyr to metarrhizium anisopliae is (1 to 60):(70 to 1). Auxiliaries and fillers are added into the effective active ingredients to form wettable powder, water dispersible granules and a suspending agent. The insecticide compound disclosed by the invention can be used for delaying the generation of the pest resistance to insecticides and can be widely applied to the field of agriculture.

The invention discloses a biofertilizer capable of increasing beneficial bacterium content in soil, which comprises the following components in parts by weight: 0.1-0.2 part of ferment bacteria, 0.1-0.2 part of bacteriophage powder, 0.1-0.2 part of Oceanospirillales powder, 0.1-0.2 part of beauveria bassiana powder, 0.1-0.2 part of Metarrhizium anisopliae powder, 20-35 parts of pig manure, 0.01-0.02 part of microelement, 5-9 parts of sodium dihydrogen phosphate, 2-7 parts of disodium hydrogen phosphate, 3-8 parts of sodium polyphosphate rice bran, 4-8 parts of wheat bran, 2-7 parts of manganese sulfate, 1-4 parts of zinc sulfate and 15-25 parts of urea. The organic fertilizer disclosed by the invention can implement plant growth, promote plant growth and inhibit various pathogenic bacteria in soil from growth by enhancing the autoimmune function of plants, promoting plant growth and improving soil.

The invention discloses a microbe-containing biofertilizer which comprises the following components in parts by weight: 0.1-0.2 part of ferment bacteria, 0.1-0.2 part of bacteriophage powder, 0.1-0.2 part of Oceanospirillales powder, 0.1-0.2 part of Beauveria bassiana powder, 0.1-0.2 part of Metarrhizium anisopliae powder, 20-35 parts of peat, 40-55 parts of chicken manure, 15-35 parts of rice bran, 10-15 parts of wheat bran, 14-24 parts of bean straw, 5-9 parts of potato straw, 0.1-0.3 part of lactobacillus powder, 0.1-0.4 part of nitrobacteria powder and 0.1-0.2 part of rhizobia powder. The organic fertilizer disclosed by the invention can implement plant growth, promote plant growth and inhibit various pathogenic bacteria in soil from growth by enhancing the autoimmune function of plants, promoting plant growth and improving soil.

The invention provides fungus insecticide. The fungus insecticide is characterized by being prepared from the following raw materials in parts by weight: 20 to 30 parts of beauveria bassiana, 10 to 20 parts of metarrhizium anisopliae, 12 to 16 parts of isaria fumosorosea, 3 to 7 parts of matrine, 8 to 12 parts of calcium dibutyl-naphthalenesulfonate citrate, 2 to 6 parts of tetramethrin, 4 to 8 parts of a liquid forming agent and 1 to 5 parts of missible oil. The fungus insecticide provided by the invention has the advantages of wide insecticidal spectrum and safety to environment.

The invention discloses a pollution-free pesticide for forestry, which is composed of the following components in parts by weight: 1-3 parts of Metarrhizium anisopliae powder, 5-6 parts of bacteriophage powder, 2-4 parts of Bacillus thuringiensis powder, 1-6 parts of herba houttuyniae, 4-9 parts of chinaberry fruit leaf, 1-6 parts of tortoise plastron, 7-11 parts of notopterygium root, 2-9 parts of polygala root, 5-8 parts of Fructus Forsythiae, 4-7 parts of radix adenophorae, 3-5 parts of Chinese wingnut leaf, 11-24 parts of pepper, 1-10 parts of synergist, 10-20 parts of stemona, 15-20 parts of chinaberry fruit, 4-10 parts of camphor, 3-4 parts of emulsifier, 1-10 parts of synergist, 12-18 parts of herba houttuyniae, 5-18 parts of creat and 100 parts of water. The green pesticide for trees and flowers is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free.

The invention discloses a pesticide composition. The pesticide composition comprises following raw materials, by weight, 25 to 30 parts of tea saponin, 15 to 20 parts of metarrhizium anisopliae, 6 to 9 parts of teflubenzuron, 10 to 13 parts of a synergist and 5 to 7 parts of an emulsifier. The synergist is capable of increasing the concentration of the pesticide composition reaching to target greatly, so as to achieve better insecticidal effect; tea saponin is extracted from plant oil meal, and possesses no toxic effect; metarrhizium anisopliae can be used for pest control of pollution-free vegetable in the field of agriculture; the combination of the raw materials is capable of reducing insect attack significantly.

The invention discloses a botanical insecticide specially used for hylocereus undulatus, and belongs to the technical field of insecticide preparation. The insecticide comprises the following raw materials: Chinese prickly ash, orange peels, African marigold, a potassium permanganate solution, tobacco, cucumber vine, tomato leaves, lightyellow sophora roots, herba coriandri, peppermint, herb of rosemary, wormwood herb, thyme, herb of French marigold, a synergist, a penetrant, a stabilizer, bacillus thuringiensis, metarrhizium anisopliae and yeast. The botanical insecticide specially used for hylocereus undulatus is prepared by extracting effective components. The insecticide provided by the invention has good insecticidal effects on common diseases and insect pests of hylocereus undulatus such as termites, snails, limax, red and yellow spiders, aphids, scarabs and drosophila melanogasters, and no insecticide resistance is generated in the insect pests.

The invention discloses a pesticide prepared by mixing destruxin and paecilomyces fumosoroseus fungus, and a preparation method thereof. The concentration of the live conidium of the paecilomyces fumosoroseus fungus in the pesticide is 10<9> to 10<11> conidia per liter, and the concentration of the destruxin is 10 mg / L to 100 mg / L; and the preparation method comprises the following steps: preparing the live conidial suspension for the paecilomyces fumosoroseus fungus, preparing the destruxin stock solution, mixing the suspension and the stock solution, and the like. The invention determines the mix proportion of the destruxin and the paecilomyces fumosoroseus fungus, obtains the better synergic effect, overcomes the defects of instable use effect and low toxicity of the single reagent of the destruxin and the paecilomyces fumosoroseus fungus, and also provides a new technical proposal for biological control. The preparation method for the pesticide is simple and feasible, and is easy to be popularized.

The invention discloses a prevention method for tobacco floating tobacco seedling spotted wilt. The prevention method comprises the steps of pretreatment and seedling management and specifically comprises the steps that spotted wilt virus hosts in seedbeds and around the seedbeds are removed, and insects are killed; blue insect sticky plates are hung in the seedbeds, 1-2 blue insect sticky plates are hung in each seedling culture small shed to monitor the dynamic state of virus transmission thrips, and insecticide is sprayed or predatory mites are released or beauveria bassiana and metarrhizium anisopliae are sprayed according to the number of the insect populations on the insect sticky plates; regular inspection or virus sampling detection is conducted, disease seedlings are removed in time when found, and an antiviral combination agent is sprayed; and 3-5 days before transplantation, 1% of tobacco seedlings are subjected to spot check, the seedbed with the disease seedling checked rate of 0.3% or above is eliminated, and systemic insecticide and antiviral inducers are sprayed once in time on qualified seedlings of the other seedbeds with the disease seedling checked rate lower than 0.3%. In the prevention method, accurate insecticide applying, insect killing and disease resistance are conducted according to the transmission characteristics and dynamic states of the thrips and the disease, insecticide usage quantity is small, thus prevention and control cost is reduced, environmental pollution is also relieved, economic benefit is increased effectively, and environment protection is improved effectively.

A process for preparing a non-woven disinfecting strip of Metarrhizium anisopliae includes sampling the bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating the Metarrhizium anisopliae to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.

The invention discloses a nutritional agent used for household planting of a camellia and relates to the technical field of household planting and cultivation of a plant. The nutritional agent is characterized by being prepared from the following materials in parts by weight: a material I: 6-9 parts of ammonium sulfate-nitrate, 9-12 parts of diammonium phosphate, 5-8 parts of zinc gluconate, 2-4 parts of humic acid, 4-7 parts of polyaspartic acid, 1-3 parts of chitosan, 5-9 parts of vitamin B powder, 3-7 parts of vitamin A powder, 2-5 parts of metarrhizium anisopliae, and 11-14 parts of anion water; a material II: 4-8 parts of carrot, 3-5 parts of juicy peach, 2-5 parts of kelp, 4-8 parts of white fungus, 3-7 parts of balsam pear, 4-7 parts of mantis, 1-4 parts of xylotrupes dichotomus, 3-5 parts of locust, 3-7 parts of bovine bone, 2-5 parts of grapefruit, and 10-13 parts of purified water; and a material III: 10-13 parts of assistant. A method is reasonable, and the agent provides rich nutrition, and is convenient and quick to prepare and convenient to use.