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Process for producing Metarrhizium anisopliae non-woven fabric ribbon

A technology of Metarhizium anisopliae and non-woven fabric bacteria, applied in botany equipment and methods, biocides, plant growth regulators, etc., can solve the problem of lack of good dosage forms and methods of use, pesticide environment and food pollution, impact Human living environment and other issues, to achieve the effect of uncomplicated production process, stable process and long-lasting control effect

Inactive Publication Date: 2003-05-28
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0002] Since the 1950s, although chemical pesticides have played a huge role in controlling pests, serious ecological consequences have followed: for example, some pest populations have increased resistance to commonly used pesticides, and the control effect has declined , the dosage of pesticides increased; a large number of natural enemies of pests were killed, causing the target pests to become rampant again and secondary pests to become major pests; some pesticide residues polluted the environment and food, affecting the living environment of human beings
Since entomogeneous fungi mainly rely on the invasion of insect epidermis, there is no good dosage form and application method for piercing-sucking mouthparts pests and borer pests

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 The production process method of the non-woven bacterial strip of Metarhizium anisopliae, comprising five production processes:

[0029] 1. Strains:

[0030] RCEF0770 bacterial strain, isolated from the beetle's Metarhizium anisopliae [Metarhizium anisopliae (Metsch.)];

[0031] 2. Culture of first-level slant bacteria:

[0032] Inoculate the isolated and purified strains on the slant of PPDA medium, each 1000ml of medium contains 200g of potato, 10g of peptone, 60g of glucose, 20g of agar, and 1000ml of water; put it into an incubator at 25°C and cultivate it for 10 days, until the mycelia cover the slant When sporulation occurs, it can be transferred to shake flask culture;

[0033] 3. Liquid seed culture:

[0034] Put 200ml of liquid seed medium into a 500ml triangular flask, sterilize it under high pressure at 1.5pa / kg for 30min, wait until it cools down to about 40°C, insert the slant bacteria, and the inoculation amount is 500ml triangular flask, t...

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Abstract

A process for preparing a non-woven disinfecting strip of Metarrhizium anisopliae includes sampling the bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating the Metarrhizium anisopliae to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.

Description

technical field [0001] The invention relates to a method for producing a new dosage form of an insecticidal fungus, in particular to a method for producing a non-woven bacterial strip of Metarhizium anisopliae (Metsch.)] RCEF0770 bacterial strain isolated from long beetles. Background technique [0002] Since the 1950s, although chemical pesticides have played a huge role in controlling pests, serious ecological consequences have followed: for example, some pest populations have increased resistance to commonly used pesticides, and the control effect has declined , the dosage of pesticides increased; a large number of natural enemies of pests were killed, causing the target pests to become rampant again and secondary pests to become major pests; some pesticide residues polluted the environment and food, affecting the living environment of human beings. Due to the large and unreasonable use of chemical pesticides, the accumulation of pesticide residues has caused the environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/08A01N63/00A01N63/04
Inventor 李增智樊美珍黄勃李农昌王四宝李春如
Owner JIANGXI TIANREN ECOLOGY
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