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Stable fungal blastospores and methods for their production, stabilization and use

A technology of blastospores and fungi, applied in the field of stable fungal blastospores and their production, stabilization and use, can solve the problems that the production of blastospores resistant to drying has not been realized, commercial use does not exist, long fermentation time and other problems

Inactive Publication Date: 2017-08-01
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC OF AGRI (US) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the commercial use of blastospores of B. bassiana is non-existent due to the long fermentation time required for blastospore preparations and poor resistance to desiccation and short spore shelf life
The production of dessication-resistant blastospores exhibiting storage stability while having bioefficacy as a biocontrol agent has not yet been achieved and is therefore desirable

Method used

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  • Stable fungal blastospores and methods for their production, stabilization and use
  • Stable fungal blastospores and methods for their production, stabilization and use
  • Stable fungal blastospores and methods for their production, stabilization and use

Examples

Experimental program
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Embodiment

[0070] Fungi and inoculum preparation

[0071] Five isolates of B. bassiana and five isolates of I. fumosorosea were tested in this study. Most of the fungal isolates originated from Brazil, details are shown in Table 1. The isolate ARSEF 3581 of I. fumosorosea and the GHA of B. bassiana (ARSEF 6444) are currently designated as (Laverlam, Butte, MT, USA) as the active ingredient of a commercial biopesticide as the US standard for liquid culture studies. Brazilian fungal isolates were previously identified using molecular techniques based on domain gene sequencing. Stock cultures of these fungi were grown on potato dextrose agar ([PDA] Detroit, MI, USA) were grown in Petri dishes at 22±2°C with a 12:12 hr (L:D) photoperiod for 2–3 weeks until sporulation and cut into 1 mm sections. 2 Agar plugs and store in 10% glycerol at -80 °C in sterile cryovials. To generate conidial inoculum, use frozen stock cultures to inoculate PDA plates and incubate for 2-3 weeks until the cul...

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Abstract

Described herein is a method for blastospore-based insect control products of entomopathogenic fungi including either B. bassiana or / . fumosowsea that produces high concentrations of stable, effective spores by identifying nutritional and environmental conditions required for the rapid production of high concentrations of a stable and infective yeast-like blastospore composition.

Description

[0001] cross reference [0002] This application claims priority to US Provisional Application No. 62 / 050,473, filed September 15, 2014. technical field [0003] The present invention generally relates to methods and compositions related to the control of arthropod pests using entomogenic fungi. Specifically disclosed are methods and compositions for producing and drying dry blastospores (vegetative cells) of Beauveria bassiana (Beauveria bassiana) and Isaria fumosorosea (Isaria fumosorosea) for controlling mollusc insect pests. Background technique [0004] Plant diseases and damage caused by arthropod pests cause enormous economic losses to plant-based agriculture and industry. A variety of approaches have been pursued to control pests that attack plants, especially commercially valuable plants. Despite this, pest damage to plants and plant products remains a major problem. [0005] Traditionally, arthropod pest control has been pursued through the application of chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N63/30
CPCC12N3/00A01N63/30A01N25/08A01N25/34
Inventor M·A·杰克森G·莫拉·马斯克林
Owner THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SEC OF AGRI (US)
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