Strain separation method of apricot ormer mushroom and cultivating method thereof
A technology of strain isolation and cultivation method, applied in the directions of botanical equipment and methods, fungi, fertilizer mixtures, etc., can solve the problems of restricting the large-scale cultivation of king oyster mushrooms, high technical requirements, complex production processes, etc., to promote ecological A virtuous circle, improving cultivation efficiency and good quality
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Embodiment 1
[0054] Weigh 200g of peeled potatoes, wash and dig out the buds and cut into thin slices, heat in water to 100°C for 30-35min, filter, take the filtrate and add 20g of agar powder to make it completely melted, then add 20g of glucose, potassium dihydrogen phosphate 1.5g and 1g of magnesium sulfate, and make up the water consumption in the original formula, keep warm at 100°C for 25-30min, filter, and control the pH value to 7-7.5;
[0055] Divide the filtered solution into test tubes, the filling volume is 1 / 4 of the test tube, plug the test tube with a cotton plug, wrap the end with the cotton plug in kraft paper, put it into an autoclave, and quickly raise it to 1.5 Sterilize under atmospheric pressure for 3 hours; or sterilize at 100°C for 10 hours under normal pressure; collect fresh fruiting bodies, and carry out epidermis disinfection, take a small piece of fruiting bodies and put them into a test tube cooled to 28°C under sterile conditions, and Place the inoculated tes...
Embodiment 2
[0057] Weigh 200g of peeled potatoes, wash and dig out the buds and cut into thin slices, heat in water to 100°C, keep warm for 30-35min, filter, add 20g of agar powder to the filtrate to make it completely melted, add 20g of glucose, and make up the original For the amount of water in the formula, keep warm at 100°C for 25-30 minutes, filter, and control the pH value to 7-7.5. Following method is with embodiment 1.
Embodiment 3
[0059] Weigh 180g of peeled potatoes, wash and dig out the buds and cut them into thin slices, put them in water or add wheat bran, heat to 100°C, keep warm for 30-35min, filter, take the filtrate and add 20g of agar powder to make it melt completely, then add 20g of glucose , Potassium dihydrogen phosphate 1.5g and magnesium sulfate 1g, and make up the water consumption in the original formula, keep warm at 100°C for 25-30min, filter, and control the pH value to 7-7.5. Following method is with embodiment 1.
[0060] original seed culture technology
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