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Method of liquid fermentation culturing flammulina velutipes mycelium rich in Se and Zn

A technology of Flammulina velutipes bacteria and liquid fermentation, which is applied in the field of bioengineering to achieve the effect of simple process, high technology content and high content of active ingredients

Inactive Publication Date: 2008-07-30
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

Zinc cannot be synthesized in the human body and can only be obtained from outside the body

Method used

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Embodiment Construction

[0046] 1. Cultivation of strains to be used: inoculate the sawdust spores on the slant of a blank eggplant bottle prepared with the above slant formula, culture at 26°C for 7-10 days, and then scrape the bacteria on the slant into the In a 250ml Erlenmeyer flask with glass beads sterilized in advance, add 30ml of sterile water and shake for 10 minutes to make a bacterial suspension. Aspirate 4ml of the bacterial suspension into two 500ml Erlenmeyer flasks that have been sterilized in advance (the culture medium prepared according to the above-mentioned primary shake flask seed formula, the liquid volume is 150ml), and cultivate it at 26°C with a rotation speed of 110r / min for 7 Tiande first-class shake flask seeds; pour one bottle of cultivated first-grade shake flask seeds into the 5000mL prepared with the above-mentioned second-grade shake flask seed formula (the filling volume is 1500mL) under aseptic operation In the Erlenmeyer flask, at 26 ℃, under the rotation speed of 1...

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Abstract

A method for cultivating golden mushroom mycelia abundant in Se and Zn elements through liquid fermentation belongs to the field of biotechnology. The invention substantially comprises cultivation of strains, cultivation of liquid seed, namely the domestication of two elements, and enrichment, fermentation and cultivation of two elements in a deep liquid, and collecting, drying and smashing of mycelia. The dried golden mushroom mycelia abundant in organic Se and organic Zn obtained with the method of the invention has over 35Mug / g organic Se and 4.25mg / g organic Zn. The powder of the mycelia with suitable food can be used as food additives and made into functional food, which has great health care function for the people in sub-health state resulted from deficient intake of microelements of Se and Zn. The invention is high in technological content, high content in effective components of the products, high additive value, which is suitable for industrial production.

Description

technical field [0001] The invention discloses a method for cultivating Flammulina velutipes mycelium rich in selenium and zinc elements by liquid fermentation, which belongs to the technical field of bioengineering. It involves the cultivation of Flammulina velutipes mycelium by fermentation method, inoculating the spores of Flammulina velutipes on a fresh solid slope for cultivation, and then inoculating them into a liquid medium containing low concentrations of inorganic selenium and inorganic zinc for seed cultivation after the slope grows well, namely Domesticate the strains; then insert them into the fermentation medium with appropriate amount of selenium and zinc for submerged liquid fermentation culture, use the enrichment and transformation functions of Flammulina velutipes to accumulate high concentrations of organic selenium and organic zinc. After the fermentation is finished, the mushroom body is collected and dried to obtain Flammulina velutipes mycelium powder ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14A23L1/212A23L19/00
Inventor 沈国强杨春霞张栋
Owner JIANGNAN UNIV
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