The invention relates to a
content determination method for
coleus forskohlii capsules. The method adopts
high performance liquid chromatography, and includes the steps of: 1. weighing an isoforskolin reference substance, adding
methanol to prepare a solution with a content of 0.1-0.3mg / ml; 2. taking
coleus forskohlii capsules, pouring out the contents, mixing the contents evenly, taking 1 part of the mixture, adding 30-100 parts of
ethanol, conducting ultrasonic treatment (or
backflow, soxhlet extraction), performing
filtration, taking subsequent filtrate, carrying out
evaporation to
dryness, or not using the aforementioned extraction method, adding 2-4 parts of
silica gel and 5-7 parts of
ethanol, blending the substances evenly, carrying out
evaporation to
dryness,
grinding the substances evenly, then transferring the substances to a
silica gel column, employing 120-180 parts of a
petroleum ether-
ethyl acetate mixed solution in a volume ratio of 3:1 to perform
elution, collecting the eluent, transferring the eluent to a
silica gel column containing 3-8 parts of 2-4% AgNO3, discarding the
effluent, using a
mixed solution of
petroleum ether and
ethyl acetate in a volume ratio of 2:1 to perform
elution, discarding the eluent, then using 90-180 parts of a
mixed solution of
petroleum ether and
ethyl acetate in a volume ratio of 3:1 to perform
elution, collecting the eluent, conducting
evaporation to
dryness, performing
dissolution with
methanol, and transferring the product into a 2-5 times measuring flask, adding
methanol to scale, and performing
filtration; and 3. sucking 5-20microl of the reference substance solution and the
test solution respectively, injecting the solutions into a liquid chromatograph to obtain a chromatogram, and calculating the content of the effective component isoforskolin in the
test solution according to the chromatogram.