Content determination of coleus forskohlii capsules

Abstract

The invention relates to a content determination method for coleus forskohlii capsules. The method adopts high performance liquid chromatography, and includes the steps of: 1. weighing an isoforskolin reference substance, adding methanol to prepare a solution with a content of 0.1-0.3mg / ml; 2. taking coleus forskohlii capsules, pouring out the contents, mixing the contents evenly, taking 1 part of the mixture, adding 30-100 parts of ethanol, conducting ultrasonic treatment (or backflow, soxhlet extraction), performing filtration, taking subsequent filtrate, carrying out evaporation to dryness, or not using the aforementioned extraction method, adding 2-4 parts of silica gel and 5-7 parts of ethanol, blending the substances evenly, carrying out evaporation to dryness, grinding the substances evenly, then transferring the substances to a silica gel column, employing 120-180 parts of a petroleum ether-ethyl acetate mixed solution in a volume ratio of 3:1 to perform elution, collecting the eluent, transferring the eluent to a silica gel column containing 3-8 parts of 2-4% AgNO3, discarding the effluent, using a mixed solution of petroleum ether and ethyl acetate in a volume ratio of 2:1 to perform elution, discarding the eluent, then using 90-180 parts of a mixed solution of petroleum ether and ethyl acetate in a volume ratio of 3:1 to perform elution, collecting the eluent, conducting evaporation to dryness, performing dissolution with methanol, and transferring the product into a 2-5 times measuring flask, adding methanol to scale, and performing filtration; and 3. sucking 5-20microl of the reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph to obtain a chromatogram, and calculating the content of the effective component isoforskolin in the test solution according to the chromatogram.

Description

[0001] Technical field: [0002] The invention relates to a drug detection method, in particular to a content determination method of sheath stamen capsules. [0003] Background technique: [0004] Chronic bronchitis is a chronic non-specific inflammation of the trachea, bronchial mucosa and surrounding tissues, clinically characterized by a chronic process of cough, sputum, or accompanied by asthma and recurrent attacks. The disease progresses slowly and is often complicated by obstructive emphysema. In severe cases, pulmonary hypertension and even cor pulmonale often occur. Primary disease is a common frequently-occurring disease, is common with middle-aged and elderly people. Although western medicine has a definite curative effect in the near future, the number of drug-resistant strains that are not sensitive to antibiotics is increasing day by day, as well as the frequent occurrence of allergic reactions and side effects of western medicine, so the treatment often fails t...

AI Technical Summary

Problems solved by technology

However, the established detection time is 80 minutes, which takes a long time to analyze and consumes a lot of reagents, which is not conducive to the analysis of multiple batches of samples

Images