Method for extracting and separating forskolin in coleus forskohlii

A technology for coleus forskohlii and crystallization, which is applied in the field of extraction and separation of terpenoid compounds, can solve the problems of complex process, low yield and high cost, and achieves the effects of simple process, high yield and low cost

Active Publication Date: 2014-02-19
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a method for extracting and isolating forskolin from the root of Coleus forskohlii, which main...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1] Extraction: the raw material of Coleus forskohlii forskohlii root is crushed, the mesh size is 60 mesh, moistened with water, and extracted with acetone after standing for 1 hour, the extraction temperature is controlled at 50°C, extracted 3 times, each time for 1.5 hours;

[0031] 2] Concentration: Combine and concentrate the three extracts in step 1 to extract, the concentration temperature is 60°C;

[0032] 3] Column separation: mix the above-mentioned extract with silica gel, put on a mixed column of silica gel and alumina, wherein the mass ratio of silica gel and alumina is 1:1.2, the mass ratio of raw materials and mixed packing is 2:1.3, n-hexane The elution ratio with ethyl acetate is 10:0.8;

[0033] 4] Crystallization: Collect and concentrate the above-mentioned crystals in sections, dissolve them with 3 times the weight of ethyl acetate, add petroleum ether 1.5 times the weight of the crystals after dissolving, and leave to crystallize for 10 hours after s...

Embodiment 2

[0035] 1] Extraction: the raw material of Coleus forskohlii forskohlii root, crushed, the mesh size is 20 mesh, moistened with lime water with a mass concentration of 5%, and extracted with ethyl acetate after standing for 10 minutes. The extraction temperature is controlled at 10°C, and the extracted 1 time, 3 hours each time;

[0036] 2] Concentration: Concentrate the extract obtained in step 1 to extract, and the concentration temperature is 40°C;

[0037] 3] Column separation: mix the above-mentioned extract with silica gel, put on a mixed column of silica gel and alumina, wherein the mass ratio of silica gel and alumina is 1:10, the mass ratio of raw materials and mixed packing is 10:1, petroleum ether Elute with a mixed solvent of ethyl acetate, and collect the eluent in sections; the ratio of petroleum ether and ethyl acetate is 10:1;

[0038] 4] Crystallization: collect and concentrate the above-mentioned crystals in sections, and dissolve them with 1 times the weight...

Embodiment 3

[0040] 1] Extraction: The raw material of Coleus forskohlii forskohlii root is crushed, the mesh size is 80 mesh, moistened with 3% acid water of mass concentration, and extracted with methanol after standing for 2 hours. The extraction temperature is controlled at 80°C, and extracted twice , 0.5h each time;

[0041] 2] Concentration: Concentrate the extract obtained in step 1 to an extract, and the concentration temperature is 80°C;

[0042] 3] Column separation: mix the above-mentioned extract with silica gel, put on a mixed column of silica gel and alumina, wherein the mass ratio of silica gel and alumina is 1:20, the mass ratio of raw materials and mixed packing is 1:1, ethyl acetate A mixed solvent of ester and acetone is used for elution, and the eluate is collected in sections; its ratio is 10:2;

[0043] 4] Crystallization: Collect and concentrate the above-mentioned crystals in sections, dissolve them with 10 times the weight of ethanol, add 0.1 times the weight of n...

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PUM

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Abstract

The invention provides a method for extracting and separating forskolin in coleus forskohlii, which solves the problems of complex process, low yield, high cost and industrialization inadequacy in the prior art. The method comprises the following steps: 1) extracting; 2) concentrating; 3) column separating and 4) crystallizating, thereby forskolin with content of more than 98% can be obtained by the above 4 steps, the yield is greater than 0.3%. The method for extracting and separating forskolin in coleus forskohlii has the advantages of simple process, high yield and low cost, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a technology for extracting and separating terpenoids, in particular to a method for extracting and separating forskolin from the root of Coleus forskohlii. Background technique [0002] Coleus forskohlii is a plant of the Lamiaceae Coleus, mainly distributed in Nepal, Bhutan, India, Sri Lanka and other places. In 1989, the rare plant was discovered and introduced in the northeast of Yunnan Province, Qi State. It is now successfully cultivated in Yunnan and Hubei. In ancient India, Coleus forskohlii was called a panacea, and it was mainly used by folks to treat diseases such as colds and coughs. It is now mostly used as a natural medicine for the treatment of tumors, cardiovascular diseases, AIDS and senile dementia. Pharmacological experiments have shown that it has a significant effect on intestinal cancer, gastric cancer, nasopharyngeal cancer, leukemia and common diseases of the elderly. [0003] At present, there are ma...

Claims

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Application Information

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IPC IPC(8): C07D311/92
CPCC07D311/92
Inventor 肖红惠战锋王晓莹张瑜
Owner SHAANXI JIAHE PHYTOCHEM
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