Supercharge Your Innovation With Domain-Expert AI Agents!

Culture medium and method for improving genetic transformation efficiency of tobacco Honghuadajinyuan

A technology of genetic transformation efficiency and safflower jinyuan is applied in the field of medium for improving the genetic transformation efficiency of tobacco safflower jinyuan, which can solve the problems of low rooting rate and low conversion efficiency of tobacco safflower jinyuan, and achieves Good differentiation, improve the efficiency of differentiation buds, and ensure the effect of selection

Inactive Publication Date: 2020-08-18
CHINA TOBACCO YUNNAN IND
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current plant transgenic technology is relatively mature, the transformation efficiency of tobacco safflower dajinyuan is generally not high, and the germination rate and rooting rate are low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium and method for improving genetic transformation efficiency of tobacco Honghuadajinyuan
  • Culture medium and method for improving genetic transformation efficiency of tobacco Honghuadajinyuan
  • Culture medium and method for improving genetic transformation efficiency of tobacco Honghuadajinyuan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: as figure 1 As shown, the method for improving the genetic transformation efficiency of tobacco safflower Dajinyuan in the present embodiment comprises the following steps:

[0038] 1. Preparation of Tobacco Safflower Dajinyuan Leaf Disc:

[0039] Take plump tobacco seeds, sterilize them in 75% alcohol for 30 seconds, then sterilize them with 10% sodium hypochlorite solution for 8-10 minutes, wash them with sterile water for 4 times, and plant the sterilized seeds on MS medium. Cultivate for 45-60 days at 25°C under 16h light / 8h dark conditions to obtain sterile tobacco seedlings;

[0040] Take the middle and upper leaves of the aseptic tobacco seedlings, under aseptic conditions, use a puncher to obtain a leaf disc with a size of 5 mm × 5 mm, soak the leaf disc in MS medium, and the MS medium is MS basic medium, Sucrose 30g / L, pH=5.75;

[0041]2. Infection of Agrobacterium: Cultivate a single colony of Agrobacterium with successfully constructed knock...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture medium and a method for improving genetic transformation efficiency of tobacco Honghuadajinyuan. The culture medium comprises a co-culture medium and a selective culture medium; the co-culture medium and the selective culture medium each comprise an MS basic culture medium, NAA and 6-BA, the final concentration of the NAA is 0.41 mg / L, and the concentration ratioof the NAA to the 6-BA is 1:5; the method comprises the following steps: preparing a tobacco Honghua Dajinyuan leaf disc; infecting with agrobacterium tumefaciens; carrying out selective culture; andcarrying out rooting culture. According to the method, the budding rate (differentiated buds / leaf discs) of tobacco calluses reaches 127%, and the rooting rate (rooted seedlings / differentiated buds) reaches 66%; compared with an original method (the budding rate is 62%, and the rooting rate is 10%), the budding rate is increased by about 2 times, and the rooting rate is increased by about 6 times;and the requirement for large-scale genetic transformation is met.

Description

technical field [0001] The invention belongs to the technical field of genetic transformation of tobacco safflower and golden element, and in particular relates to a culture medium and a method for improving the genetic transformation efficiency of tobacco safflower and golden element. Background technique [0002] cultivated tobacco ( Nicotiana tabacum ) is an allotetraploid belonging to the family Solanaceae, and is considered an important model organism for verifying gene functions in other plants due to its easy genetic transformation. Compared with the model plant Arabidopsis thaliana, there is still a big gap in the functional research of tobacco genes. In recent years, the emergence of CRISPR / Cas9 technology has provided an effective tool for the study of tobacco gene function. In order to quickly obtain more tobacco mutants, a knockout plasmid library based on CRISPR / Cas9 can be used for mixed transfection to speed up the pace of tobacco gene function research. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/04C12N15/82A01H4/00A01H5/00A01H6/82
CPCA01H4/001A01H4/008C12N5/0025C12N15/8205C12N2500/30C12N2500/40
Inventor 蒋佳芮向海英曾婉俐高茜许力张建铎邓乐乐杨文武宋春满贾凌李雪梅杨光宇陈章玉夏庆友
Owner CHINA TOBACCO YUNNAN IND
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com