Chinese narcissus callus preservation and tissue culture method

A callus, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, to achieve the effects of fast reproduction, vigorous growth, and easy differentiation

Inactive Publication Date: 2013-04-03
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for tissue culture of callus preservation in view of the problem that the Chinese narcissus callus is difficult to subculture and preserve in the prior art, and to cultivate good callus

Method used

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  • Chinese narcissus callus preservation and tissue culture method
  • Chinese narcissus callus preservation and tissue culture method
  • Chinese narcissus callus preservation and tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the tissue culture method of Chinese narcissus callus preservation

[0031] The tissue culture method of Chinese narcissus callus preservation, comprises the following steps:

[0032] 1. Material selection: Take Chinese Narcissus ovaries in the flowering stage and free from diseases and insect pests, soak them in alcohol with a volume ratio of 75% for 30 seconds, then disinfect them with 0.1% mercury liter by weight for 5 minutes, and finally rinse them with sterile water for 3 times ;

[0033] 2. Induction culture: On the ultra-clean bench, cut the sterilized ovary into 5 mm thick slices and inoculate them with MN+4.0 mg / L 6-BA+0.4 mg / L 2,4-D+0.2 mg / L NAA + 30g / L sugar + 6 g / L agar powder, on the induction medium with pH 5.8; the temperature of the culture room is 25°C-28°C, the light is 12 h / d, and the light intensity is 1200 lx-1500 lx; ​​culture for 60 days;

[0034] 3. Preservation of callus: The formula of callus preservation medium is MN+2.0 mg / L...

Embodiment 2

[0035] Embodiment two, the tissue culture method of Chinese narcissus callus preservation

[0036] The tissue culture method of Chinese narcissus callus preservation, comprises the following steps:

[0037] 1. Material selection: refer to the material selection in Example 1.

[0038] 2. Induction culture: refer to the induction culture in Example 1.

[0039] 3. Preservation of callus: The formula of callus preservation medium is MN+3.0 mg / L 6-BA+1.5 mg / L 2,4-D+0.4 mg / L NAA+30 g / L sugar+6 g / L Agar powder, pH 5.8; callus cut into 0.5 cm 2 After the small pieces were inoculated into the callus preservation medium; cultured for 60 days under the conditions of the temperature of the culture room at 25°C-28°C, light of 12 h / d, and light intensity of 1200 lx-1500 lx, the callus The average reproduction coefficient reached 3.19 times, but the callus grew slowly.

Embodiment 3

[0040] Embodiment three, the tissue culture method of Chinese narcissus callus preservation

[0041] The tissue culture method of Chinese narcissus callus preservation, comprises the following steps:

[0042] 1. Material selection: refer to the material selection in Example 1.

[0043] 2. Induction culture: refer to the induction culture in Example 1.

[0044] 3. Preservation of callus: The formula of the callus preservation medium is MN+1.0 mg / L 6-BA+0.5 mg / L 2,4-D +0.4 mg / L NAA+30 g / L sugar+6 g / L L agar powder, pH 5.8, cut the callus into 0.5 cm 2 After the small pieces were inoculated into the callus preservation medium; cultured for 60 days under the conditions of the temperature of the culture room at 25°C-28°C, light of 12 h / d, and light intensity of 1200 lx-1500 lx, the callus The average reproduction coefficient reaches 3.93 times.

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Abstract

The invention relates to a Chinese narcissus callus preservation and tissue culture method. The method comprises the steps of material selection, induction culture and callus preservation. When a Chinese narcissus callus preservation and culture medium provided by the invention is put into use, the reproduction coefficient of Chinese narcissus callus can be 5.16 times, and the callus grows well, has a high reproduction speed and is free from browning and differentiation phenomena. According to the Chinese narcissus callus preservation and tissue culture method, the problems of difficult subculture and preservation, low reproduction coefficient, serious browning and differentiation and the like of the Chinese narcissus callus can be solved, the practicability is high and the generalization performance is good. The callus preserved by the method is easy to differentiate when being transferred to a differential medium, and can serve as an excellent material of a receptor for genetic transformation of Chinese narcissus.

Description

technical field [0001] The invention relates to a method for tissue culture of Chinese narcissus, in particular to a method for preserving tissue culture of Chinese narcissus callus. Background technique [0002] Chinese Narcissus ( Narcissus tazetta L. var. chinensis Roem) Amaryllidaceae Narcissus is a perennial bulbous flower. It is one of the top ten traditional flowers in my country and one of the flowers that are displayed indoors in winter in the world. It has high ornamental value, economic value and medicinal value. At present, the Chinese narcissus used for production and cultivation mainly includes two cultivars, 'Jinzhan Yintai' and 'Yulinglong'. The speed is slow, and the Chinese narcissus is triploid, so it is difficult to produce new varieties by natural or artificial hybridization, resulting in a single variety and few varieties. In addition, it is susceptible to virus infection during the cultivation process, resulting in a decline in quality and degene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 冯莹潘东明
Owner FUJIAN AGRI & FORESTRY UNIV
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