33 results about "Vernonia amygdalina" patented technology

The invention discloses a tissue culture rapid-propagation seedling culture method of vernonia amygdalina, comprising the following steps: (1) collecting of an explant: collecting a tender stem, removing leaves, and flushing by using clean water, wherein the flushed stem is taken as the explant for standby application; (2) disinfecting of the explant: soaking and disinfecting the explant obtained in the step (1), and flushing by using sterile water; (3) primary culture: inoculating the disinfected explant obtained in the step (2) into an induction medium for culture; (4) subculture: separating and cutting adventitious buds formed in the induction medium in the step (3), and transferring into a subculture medium for culture; (5) rooting culture: selecting robustproliferated seedlings obtained in the step (4), and transferring into a rooting culture medium for culture; (6) transplanting: after root development is finished, washing off a base culture medium, exercising seedlings for 10 days, and then transplanting into a light matrix. The method has the advantages that the seedling propagation speed is fast, the production is not affected by seasons, the transplanting survival rate is high, the seedling consistency is good, and the like, and a technique is provided for large-scale standardized production of the vernonia amygdalina.

The invention provides a method for culturing inonotus hispidus by using vernonia amygdalina, and belongs to the technical field of edible fungi cultivation. A culture medium is used in the method forculture; a solid culture medium includes the following components by the mass percentage: 80%-100% of cottonseed hull, 0%-20% of corncob, and 0%-20% of rice, and the sum of the mass percentages of all the components is 100%. A nutrient liquid culture medium includes the following components: 0.045%-0.055% of vernonia amygdalina powder, 1.8%-2.2% of glucose, 0.15%-0.25% of yeast extract powder, and the balance water, wherein the sum of all the components is 100%, and the pH is neutral; the solid culture medium and the liquid culture medium are mixed according to the mass ratio of 1:1.4-1:1.6.The mycelia of inonotus hispidus cultured by the culture medium grow fast, the fruiting body growth cycle is short, and the culture medium is simple to prepare, easy to operate and prone to realization of industrialization.