Method for improving yield of lucid ganoderma exopolysaccharides
An extracellular polysaccharide and Ganoderma lucidum cell technology is applied in the field of Vernonia almondii leaf powder to increase the yield of Ganoderma lucidum liquid fermentation exopolysaccharide, which can solve the problems of unstable quality of active ingredients, low production efficiency and the like, and achieve the enhancement of biological activity and pharmacological function. Effect
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Embodiment 1
[0017] Example 1 Adding 5g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide
[0018] Put the ganoderma lucidum mycelia stored on the PDA slope at 4°C at room temperature for 4 hours, then pick the mycelium with a size of 5mm×5mm on the slope and insert it into a fresh PDA plate, culture at 30°C for 7 days, the plate is covered with The mycelium is activated. Use a sterilized toothpick to pick out mycelium with a size of 5 mm × 5 mm, insert it into a 50 mL centrifuge tube containing 10 mL of seed medium, and culture it on a shaker at 28 ° C and 150 rpm for 7 days. In the ultra-clean workbench, next to the alcohol lamp, pour the well-cultivated seed culture solution (10ml per bottle) into a 250mL Erlenmeyer flask containing 100mL of fermentation medium added with Vernonia almond leaf powder. The addition amount of leaf powder is 5g / L. Place on a shaker at 28°C and 150 rpm for 8 days.
[0019] The obtained fermented liquid was transferre...
Embodiment 2
[0022] Example 2 Adding 10g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide
[0023] Other conditions were the same as in Example 1, except that the amount of Vernonia almond leaf powder was adjusted to 10 g / L. The result shows that the ganoderma mycelium biomass is 12.3g / L, and the ganoderma exopolysaccharide output is 3.32g / L (see figure 1 ).
Embodiment 3
[0024] Example 3 Adding 15g / L Almond Vernonia leaf powder to ferment and produce Ganoderma lucidum exopolysaccharide
[0025] Other conditions were the same as in Example 1, except that the addition of Vernonia almond leaf powder was adjusted to 15g / L. The results showed that the biomass of Ganoderma lucidum mycelium was 10.9g / L, and the yield of exopolysaccharide of Ganoderma lucidum was 3.04g / L.
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