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The Rapid Propagation Method of Vernonia almonds

A kind of Almond Vernonia, fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult cleaning of medium, degradation of breeding seedlings, and reduced survival rate, etc., to overcome the degradation of breeding seedlings, reduce Diseases and the effect of reducing the survival rate

Active Publication Date: 2017-09-01
SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation process of cutting technology, diseases will accumulate year by year, causing the propagation seedlings to degenerate; if tissue culture is adopted, when the rooted seedlings are taken out from the medium, the medium on the roots of the seedlings is not easy to clean, which is labor-intensive, and the medium is rich in nutrients. During the hardening and planting process, it is easy to be infected with germs and reduce the survival rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Propagate Almond Vernonia by following these steps:

[0032] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for about 1 hour, soak in alcohol with a volume ratio of 75% for about 25 seconds on the ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 1g / L mercury chloride solution for about 9 minutes, rinse with sterile water After 3 times, inoculate in MS starting medium containing 1mg / L 6-BA and 0.1mg / L NAA, culture temperature is 25°C, light intensity is 1500LX, light time is 13h per day;

[0033] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, inoculate them into MS proliferation medium containing 1mg / L6-BA and 0.1mg / LNAA, the temperature is 25℃, the light intensity is 1500LX, and th...

Embodiment 2

[0039] Propagate Almond Vernonia by following these steps:

[0040] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for 0.5h, soak in alcohol with a volume ratio of 75% for 20s on the ultra-clean workbench, rinse with sterile water twice, then disinfect with 1g / L mercury chloride solution for 10min, rinse with sterile water twice Afterwards, inoculate in the MS starting medium containing 0.5mg / L 6-BA and 0.05mg / L NAA, the culture temperature is 23°C, the light intensity is 2000LX, and the light time is 10h per day;

[0041] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, inoculate them into MS proliferation medium containing 0.5mg / L6-BA and 0.05mg / LNAA, the temperature is 23°C, and the light intensity is 2000LX. The ligh...

Embodiment 3

[0047] Propagate Almond Vernonia by following these steps:

[0048] (1) Induction of explants: Stems were cut from Vernonia amygdala plants, which were growing robustly and free from diseases and insect pests, as explants, soaked in a diluted solution of detergent, and then cleaned the surface of the explants with a brush. Then rinse with running water for 0.8h, soak in alcohol with a volume ratio of 75% for 30s on the ultra-clean workbench, rinse with sterile water for 4 times, then disinfect with 1g / L mercury chloride solution for 8min, and rinse with sterile water for 4 times Afterwards, inoculate in the MS starting medium containing 1mg / L 6-BA and 0.05mg / L NAA, the culture temperature is 28°C, the light intensity is 1500~2000LX, and the light time is 15h per day;

[0049] (2) Subculture of induced buds: when the induced buds grow to 2~3cm, they are inoculated into MS proliferation medium containing 0.5mg / L6-BA and 0.1mg / LNAA, the temperature is 28℃, and the light intensity...

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Abstract

The invention discloses a propagation method of Vernonia amygdala. Specifically, the secondary buds of Vernonia amygdala are placed in a rooting medium for pre-cultivation to obtain aseptic seedlings, and then the aseptic seedlings are cut into the substrate for cultivation. After rooting, Plant in pots after new leaves have grown. The present invention combines tissue culture technology and cutting technology to propagate Vernonia amygdala to achieve complementary advantages of the two. The pre-cultivated and unrooted aseptic seedlings are cut into the mixed matrix of peat soil, river sand and vermiculite to take root, which overcomes the need for cleaning and cultivation. It is helpful to improve the survival rate due to the problems of root and susceptibility to pathogenic bacteria; tissue culture technology can be used to provide large-scale cutting propagation materials all year round, meeting the requirements of large-scale production and annual production of seedlings.

Description

technical field [0001] The invention relates to a propagation method of Vernonia amygdala, in particular to a rapid propagation method of Vernonia amygdala in combination with tissue culture and cutting techniques. Background technique [0002] Vernonia amygdalina Del., also known as Vernonia amygdalina, Vernonia apricot leaf, magic tree, bitter tree, South African tree, South African leaf, sharp tail phoenix, etc., is a plant of the genus Vernonia in the family Asteraceae, native to Africa, most common in West Africa. At present, there are about 1,000 species of Vernonia plants in the Asteraceae family, most of which belong to herbs, woody vines or trees, and mainly grow in tropical regions. About 30 species have been found in my country, including Vernonia rugosa, Vernonia willow, Vernonia repellent, and Nocturnal oxen, etc. The main species are distributed in southwest to southeast, Taiwan, Xinjiang and other places. Vernonia amygdala is mostly a perennial tree or shrub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 沈彦会周桂英许建新刘文竹唐彪赵克奇刘璞张静王成聪
Owner SHENZHEN TECHAND ECOLOGY & ENVIRONMENT CO LTD
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