54results about How to "Accelerate self-renewal" patented technology

The present invention provides a preparing method of a human hepatic progenitor cell which is amplified and cultivated in vitro, including: a. separating the human hepatic progenitor cell; b. co-cultivating a feeder cell and the human hepatic progenitor cell separated from the step a by a medium having no serum on an extracellular matrix containing human fibrin sealant or other analogues, obtaining a human hepatic progenitor cell colony by amplification. The human hepatic progenitor cell colony is easy to separate from the surface of human fibrin sealant by the simple gelatinolytic band process, purified or / and subcultured by further single cell preparation technology process such as enzymatic degradation etc. The human hepatic progenitor cell prepared by the method is hepatic progenitor cell treatment, including a cell transplantation and a bioartificial liver support system, providing excellent human hepatocyte source for cytotoxicity test platform in the drug screening, virus infection and drug screening platform etc.

The present invention discloses cell permeable Nanog and Oct4 recombinant proteins that comprise a kaposi fibroblast growth factor 4 (kFGF4)-derived macromolecule transduction domain (MTD). Also disclosed are polynucleotides encoding the cell permeable Nanog and Oct4 recombinant proteins, a method of increasing self-renewal and suppressing differentiation of stem cells by treating the cells in combination with the cell permeable Nanog and Oct4 recombinant proteins, and the combined use of the cell permeable Nanog and Oct4 recombinant proteins for increasing self-renewal and suppressing differentiation of stem cells.

The invention relates to a skin barrier repairing composite and application thereof. The skin barrier repairing composite comprises sodium pyrrolidonecarboxylate, ecdysterone, an itching relieving anti-allergy composite and a metabolic balance composite, wherein the itching relieving anti-allergy composite comprises a radix stephaniae tetrandrae extractive, potassium lactate, dipotassium glycyrrhizinate and phenoxyethanol; the metabolic balance composite comprises magnesium aspartate, zinc gluconate and copper gluconate. According to the skin barrier repairing composite, the sodium pyrrolidonecarboxylate and the ecdysterone are adopted to repair the skin barrier, the metabolic balance composite (M3) is adopted to improve cell activity and accelerate metabolic balance, the itching relievinganti-allergy composite (Comthing tetra) is adopted to lower itching and scratching caused by inflammation or histamine release, the skin barrier is protected from mechanical injury, the scalp barrierfunction is effectively recovered, and the susceptibility of the scalp is lowered.

The invention relates to the field of molecular biology and discloses an ovarian cancer stem cell vaccine and a preparation method and application thereof. Cancer stem cells are insensitive to radiotherapy and chemotherapy, which is the root of cancer metastasis and recurrence. Aiming at active immunotherapy of the cancer stem cells, satisfactory therapeutic effect can be gained only on the condition that cancer 'seed' cells, namely the cancer stem cells are eliminated. The ovarian cancer stem cell CD117+CD44+ vaccine is capable of improving blood serum IFN (interferon)-gamma level, lowering TGF (transforming growth factor)-beta expression, enhancing NK cell activity, decreasing the number of ovarian cancer stem cells CD117+CD44+ remarkably and inhibiting cancer growth in animal experiments.

The invention discloses a method and a device for updating software. The method is implemented by the aid of an updating module on the basis of software. The method includes steps of enabling the updating module to acquire a version of the software; connecting the updating module with a network and acquiring the latest version of the software; enabling the updating module to compare the version of the software to the latest version of the software; connecting the updating module with the network to download software in the latest version if the latest version of the software is higher than the version of the software, and updating the software. The method and the device for updating the software in an embodiment of the invention have the advantages that the updating software is arranged on the outside of the software, so that the version and information of the software can be independently acquired by the updating module without being affected by the software, the software still can be updated under the condition that the software fails or is collapsed, the self-updating and repairing ability of the software can be improved, and the user experience can be enhanced.

The invention provides a sunblocking cream which is natural and resists ultraviolet B (UVB) radiation and ultraviolet A (UVA) radiation, and can improve the resisting and defending capacity of the human body cells to ultraviolet radiation. The product comprises the following raw materials in parts by weight: 0.5-5 parts of zingiber corallinum hance essential oil, 0.5-1 parts of curcuma oil, 0.5-1parts of zedoary turmeric oil, 5-8 parts of emulsifier, 1-5 parts of humectant, 1-3 parts of active agent, 8-10 parts of excipient and 62.8-72.3 parts of pure water, and is prepared by the following steps: mixing and heating aqueous phase substances, namely the humectant, active agent and pure water to the temperature of 85-87 DEG C; evenly mixing and heating oil phase substances, namely the zingiber corallinum hance essential oil, curcuma oil, zedoary turmeric oil, emulsifier and excipient to the temperature of 85-87 DEG C; and slowly pouring the oil phase into the aqueous phase, stirring and evenly mixing, homogenizing for 5-10 minutes at the temperature of 85-87 DEG C, and cooling. The sunblocking cream of the invention resists the UVB radiation and UVA radiation, can improve the resisting and defending capacity of the human body cells to ultraviolet radiation, can promote skin cell division and accelerates self renewal of the skin cells; the skin does not generate erythema after being exposed to the sun, and can not be tanned; and the sunblocking cream has good sunblocking effect, low application cost, simple and feasible preparation method.

The invention belongs to the technical field of cosmetics, and particularly relates to facial mask essence containing chitosan and a preparation method thereof. The facial mask essence is prepared from the following raw materials in percentage by mass: 0.5-5 percent of chitosan, 0.5-5 percent of yeast beta-glucan, 1-15 percent of marine collagen, 3-8 percent of glycerinum, 0.05-0.2 percent of allantoin, 0.05-0.2 percent of bletilla striata polysaccharide, 1-10 percent of plant extract, 0.000001-0.0003 percent of growth factor and 50-95 percent of deionized water. The facial mask essence containing chitosan is mild in property, does not irritate skin, has the advantage that the active ingredient can be easily absorbed by skin so as to accelerate self-renewal and repair of skin cells, has remarkable effects of relieving senescence, moistening, diminishing inflammation, alleviating allergy and whitening skin, and can be used for quickly improving skin texture, increasing skin elasticity, smoothing wrinkles and the like.

The invention provides a functional lipstick which has broad antibacterial spectrum, has the functions of antiphlogosis, detumescence and analgesia, is used for treatment or prevention, and has short treatment period, low cost and simple and practical preparation method. The preparation method comprises the following steps: weighting the following raw materials in parts by weight: 0.5-3 parts of Zingiber corallinum Hance essential oil, 10-15 parts of tea tree oil, 10-15 parts of castor oil, 10-15 parts of beeswax, 8-10 parts of paraffin oil, 20-40 parts of essential oil, 10-20 parts of glyceryltri(2-ethylhexanoate) and 2-6 parts of lanolin; heating tea tree oil, castor oil, beeswax, paraffin oil, lanolin and glyceryltri(2-ethylhexanoate) for eutectic melting, stirring evenly, cooling to 35-45 DEG C, adding Zingiber corallinum Hance essential oil, fully and evenly stirring, and cooling to obtain the finished product. The invention has remarkable effects of antiphlogosis, detumescence and analgesia, improves the microcirculatory disturbance caused by the inflammation of the lip, promotes the blood circulation and the self-renewing and self-repairing abilities of tissues, takes effect fast, and has short treatment period, high recovery rate and no toxic or side effects.

The invention relates to a micro-needle based in vitro culture method for testicular tissue and applications thereof, and belongs to the technical field of biology. The method for in vitro culturing testicular tissue to promote proliferation of spermatogonial stem cells is provided. The method includes: soaking PEGDA micro-needles in a culture medium for replacement, so as to obtain a culture system including replaced PEGDA micro-needles and the culture medium; and adding isolated testicular tissue in the culture system to culture, so as to proliferate the spermatogonial stem cells in the isolated testicular tissue. The method improves situations of death of tissue centers during tissue culture in vitro, so that large tissue culture can be realized. Therefore, a physiological structure of the tissue can be maintained to the greatest extent, which is more in line with in vivo situations. In addition, the method can promote the proliferation of the spermatogonial stem cells in vitro, the self-renewal of the spermatogonial stem cells (SSCs) is supported, and the dryness of the spermatogonial stem cells can be maintained.

The present invention provides a facial mask with excellent moisturizing and repairing effects, the components of which include a polypeptide and a moisturizing agent. Mixed active protein peptides obtained by mixing the small molecule penetrating peptides shown in 3. The mask formula of the present invention is compounded with TAT-EL mixed active protein peptide: it replaces tissue / collagen / capillary to receive free radical attack, so as to protect capillaries and collagen; it has excellent functions of promoting cell proliferation and differentiation, accelerating The self-renewal and self-repair of skin epidermal cells has a good effect on the repair of damaged acne muscles / medical and cosmetic wounds. The acetylated hyaluronic acid used in the present invention, as one of the moisturizing agents, enhances its solubility, permeability and resistance to hyaluronidase; It is also soluble in oil, and can use the intercellular substance as a carrier to penetrate into the basal layer of the skin and exert a moisturizing effect.

The invention discloses culture fluid for maintaining the pluripotency of human amniotic epithelial stem cells. The culture fluid comprises base culture fluid, 15%-25% of serum substitutes, 0.1%-2% of non-essential amino acid, 1 mmol / L-5 mmol / L of essential amino acid, 0.01 mmol / L-1 mmol / L of dithiothreitol, 30 ng / ml-120 ng / ml of growth factors and 0.1%-2% of system stabilizing factors. The culture fluid has the advantages that in-vivo survival environments of the stem cells can be simulated by the culture fluid, accordingly, the pluripotency of the stem cells can be maintained when the stem cells are subjected to in-vitro culture, and stem cells with high pluripotency still can be obtained after repeated pass culture is carried out.

The present invention relates to the cosmetic use of dedifferentiated plant cells of Rosa plants or of extracts or lyophilizates of said cells as active agents for the care of aging skin or aging hair. The present invention also relates to rose dedifferentiated cells, especially rose dedifferentiated cells of Lancome.

The invention discloses application of a substance for inhibiting TGF-betaR1 expression in treatment and / or prevention of esophageal cancer. It finds that TGF-betaR1 is highly expressed in esophageal cancer stem cell spheres and can promote proliferation and self-renewal of esophageal squamous carcinoma cells and formation, migration and invasion of tumor stem cell spheres, and substances for inhibiting expression of TGF-betaR1 can reverse the phenomenon. The invention further provides a theoretical basis and an experimental basis for defining the occurrence and development mechanism of esophageal cancer and targeted therapy.

The present invention discloses a method for preparing a human hepatic progenitor cell which is amplified and cultivated in vitro, including: a. separating the human hepatic progenitor cell; b. co-cultivating a feeder cell and the human hepatic progenitor cell separated from the step a by a medium having no serum on an extracellular matrix containing human fibrin sealant or other analogues, obtaining a human hepatic progenitor cell colony by amplification. The human hepatic progenitor cell colony is easy to separate from the surface of human fibrin sealant by the simple gelatinolytic band process. The human hepatic progenitor cell can be purified or / and subcultured by further single cell preparation technology process such as enzymatic degradation etc. The human hepatic progenitor cell prepared by the method provides good human hepatic progenitor cell source for hepatic cell treatment, including a hepatic cell transplantation and a bioartificial liver support system, cytotoxicity test platform in the drug screening, hepatitis virus infection and drug screening platform and the like.