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Medicine for inhibiting proliferation of esophageal cancer cells

A technology for cancer cells, esophageal squamous cell carcinoma, applied in the biological field

Inactive Publication Date: 2018-06-22
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of SOX9 in different tumors is not completely the same; in prostate cancer, highly expressed SOX9 can inhibit the growth of tumor cells, which indicates that the different roles of SOX9 may be produced by regulating different target genes

Method used

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  • Medicine for inhibiting proliferation of esophageal cancer cells
  • Medicine for inhibiting proliferation of esophageal cancer cells
  • Medicine for inhibiting proliferation of esophageal cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1. High expression of SOX9 in esophageal squamous cell carcinoma tissue and esophageal cancer stem cell spheres

[0066] 1. Tissue specimens

[0067] The tissue samples of the present invention come from the First Affiliated Hospital of Shihezi University School of Medicine, Xinjiang Yili Friendship Hospital and Xinjiang Autonomous Region People's Hospital, including 195 cases of esophageal squamous cell carcinoma and paracancerous tissue samples. The above-mentioned specimens were obtained with the prior informed consent of the subjects.

[0068] 2. Detection of SOX9 expression in tissue microarray samples by immunohistochemical method

[0069] The expression of SOX9 in tissue microarray samples was analyzed by immunohistochemical method. The specific steps are as follows: After the paraffin tissue chip is routinely sectioned, dewaxed, and xylene removed, the antigen is repaired by the method of heat repair antigen; take out the repair box and let it stand at...

Embodiment 2

[0083] Example 2. Preparation of esophageal cancer cells with reduced expression of SOX9 and application of substances that interfere with SOX9 expression in inhibiting proliferation, EMT and self-renewal of esophageal cancer cells

[0084] 1. Expression of SOX9 protein in esophageal squamous cell carcinoma cell line

[0085] The expression of endogenous SOX9 protein in esophageal squamous cell carcinoma cell lines EC9706, ECa-109, KYSE150 and TE-1 was detected by western blot. The detection method is the same as Step 4 in Example 1.

[0086] The results showed that the expression of SOX9 protein was the lowest in the ECa-109 cell line, and it was expressed to varying degrees in the other three cell lines ( figure 2 -A). Finally, the cell line EC9706 with higher expression of endogenous SOX9 was selected for subsequent experiments related to inhibition of SOX9, and the cell line ECa-109 with the lowest expression of endogenous SOX9 was selected for subsequent experiments re...

Embodiment 3

[0111] Example 3. Preparation of SOX9 Overexpression Cells and Application of SOX9 in Promoting Proliferation, EMT, Self-renewal and Stem Cell Characteristics of Esophageal Cancer Cells

[0112] 1. Preparation of SOX9 overexpression cells

[0113] 1. Lentivirus overexpressing SOX9

[0114] The lentivirus overexpressing SOX9 was constructed by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0115] 2. Cells transfected with lentivirus

[0116] The lentivirus overexpressing SOX9 was inoculated in the ECa-109 cell line, and the lentiviral vector vector (constructed by Shanghai Gemma Pharmaceutical Technology Co., Ltd.) was used as a control. Cells successfully transfected with virus.

[0117] 3. Western blot detection

[0118] Western blot was used to detect the expression of SOX9 protein in SOX9 overexpression cells (SOX9) and control cells (Vector).

[0119] The results showed that the expression of SOX9 protein in the SOX9 overexpression cells (SOX9) was significantly...

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Abstract

The invention discloses a medicine for inhibiting proliferation of esophageal cancer cells. The roles of SOX9 in the occurrence and development of esophageal squamous cell carcinoma and a downstream molecular mechanism regulated by the SOX9 are researched from the view of tumor stem cells by detecting clinical specimens and establishing an in vitro cell model and an in vivo mouse model; experiments proves that the SOX9 is highly expressed in the tissues of the esophageal squamous cell carcinoma and in esophageal cancer stem cell balls, and can promote the proliferation and self-renewal of a cell line of the esophageal squamous cell carcinoma, the formation of tumor stem cell balls, the growth of transplantation tumor in nude mice, and the tolerance to chemotherapeutic drug cisplatin. The invention provides a theoretical basis and an experimental basis for further clarifying the occurrence and development mechanism and targeted treatment of esophageal cancer.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to drugs for inhibiting the proliferation of esophageal cancer cells, in particular to the application of substances that interfere with the expression and / or activity of SOX9 in the preparation of products for inhibiting the proliferation of esophageal cancer cells. Background technique [0002] Esophageal cancer is the sixth leading cause of cancer-related deaths. Most of esophageal cancer occurs in developing countries, especially in China, where the incidence of esophageal cancer is about 70% of the total number of esophageal cancer in the world. The five-year survival rate for esophageal cancer is only about 15%, and most patients die within a year of diagnosis. Due to the lack of specific and sensitive early diagnostic indicators and methods, nearly half of the patients with esophageal cancer have metastasized at the time of diagnosis, thus losing the best opportunity for surgica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/713A61P35/00A61P35/04
CPCA61K31/713A61K45/00
Inventor 王良海张之钰侯隽郁晓丹庞丽娟李锋
Owner SHIHEZI UNIVERSITY
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