45 results about "Placental blood" patented technology

The present invention provides an improved kit for the collection of umbilical cord blood and placental blood, and collection of the placenta from which such blood is obtained. The kit improves upon existing kits in that it provides for improved user convenience, provides for the collection of the placenta itself, and better maintains the internal temperature of the container in which the collected blood and placenta are shipped to a blood bank or registry. The invention further provides a method of collecting umbilical cord and placental blood, and the placenta from which such blood is obtained, comprising using the kit described herein.

The present invention provides an improved kit for the collection of umbilical cord blood and placental blood, and collection of the placenta from which such blood is obtained. The kit improves upon existing kits in that it provides for improved user convenience, provides for the collection of the placenta itself, and better maintains the internal temperature of the container in which the collected blood and placenta are shipped to a blood bank or registry. The invention further provides a method of collecting umbilical cord and placental blood, and the placenta from which such blood is obtained, comprising using the kit described herein.

The present invention provides an improved kit for the collection of umbilical cord blood and placental blood, and collection of the placenta from which such blood is obtained. The kit improves upon existing kits in that it provides for improved user convenience, provides for the collection of the placenta itself, and better maintains the internal temperature of the container in which the collected blood and placenta are shipped to a blood bank or registry. The invention further provides a method of collecting umbilical cord and placental blood, and the placenta from which such blood is obtained, comprising using the kit described herein.

The present invention provides an improved kit for the collection of umbilical cord blood and placental blood, and collection of the placenta from which such blood is obtained. The kit improves upon existing kits in that it provides for improved user convenience, provides for the collection of the placenta itself, and better maintains the internal temperature of the container in which the collected blood and placenta are shipped to a blood bank or registry. The invention further provides a method of collecting umbilical cord and placental blood, and the placenta from which such blood is obtained, comprising using the kit described herein.

A system and method are provided for analyzing medical images and monitoring pregnancies for a plurality of animal patients. Medical images obtained from medical imaging devices are scanned, and extracted data are stored in a database. A color histogram is generating characterizing the image based on placental blood flow.

A method for preserving whole placental blood comprising introducing whole placental blood into an air barrier storage bag, storing said bag containing whole placental blood at a temperature of more than 0° C. and less than 40° C., so as to preserve the whole placental blood.

Provided herein are dual modality imaging systems and methods within displayed anatomical structures of placenta in real time. The imaging system comprises a dual modality laser optoacoustic and ultrasonic platform with a plurality of subsystems for delivering near infrared light, optoacoustic and ultrasonic pulses to the placenta and / or associated tissue and deep anatomic structures, for detecting ultrasonic pulses generated or reflected within the tissue using a multi-channel optoacoustic-ultrasound probe and associated transducers. The dual modality imaging system displays the results obtained as quantitative functional images of the parameters coregistered with anatomic tissue images. A multichannel electronic system comprising a computer tangibly storing software enables processor-executable instructions to calculate quantitative functional parameters of the placental blood within specific anatomical tissue structures and display quantitative functional optoacoustic images of the functional parameters within specific anatomical structures in the tissue that are visualized by ultrasound.

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL / mL, human Source AB type serum 5‑15 μL / mL, human transferrin 5‑15 μg / mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.

The present invention relates to a method for isolating mesenchymal stem cells from placental vessels and used digestive enzyme compositions. The method comprises the following steps: the placenta issterilized with alcohol; Dissecting placental blood vessels from the placenta; Cut into pieces, wash with PBS, filter the residual blood stains to obtain placental vascular tissue; Adding mixed enzymesolution to digest; End digestion, filter and collect tissue fluid; The placental mesenchymal stem cells (P0) were obtained by centrifugation and cultured with DMEM-F12 basal medium was resuspended and the number and viability of nucleated cells were counted. The obtained cells are cryopreserved with cryopreservation protection solution so as to be re-cultured before use, or the passages and / or the mesenchymal stem cells are continued to be subjected to cell identification and / or detection, cryopreservation, library establishment and the like. The method of the invention can effectively improve the efficiency of isolating mesenchymal stem cells from the blood vessels of the placenta.

The invention provides a method for preparing CD3+CD56+cells through high-killing K562 cells from perinatal placental blood. The method comprises the step of: separating mononuclear cells from the perinatal placental blood so as to carry out induction differentiation to form CD3+CD56+cells. By utilizing the method, the preparation period of the cells can be shortened, and moreover, the multiplication capability of the cells is improved.

The invention discloses a cell culture solution for enhancing cartilage differentiation induction, a method and an application. A blood platelet lysate is extracted and prepared from clinically wasteplacental blood, so that the source is wide, and the cost is low. Mesenchymal stem cells obtained by the method can stably grow in an adherent manner, characteristics are similar to those of cells obtained by a conventional culture method, and the cells are in a typical fusiform vortex shape under a microscope; and compared with the conventional culture method, the method has the advantages that expression of cartilage differentiation marker genes SOX9 and COL2A1 in the cells can be effectively activated, the number and strength of formed cartilages after in-vitro induction are obviously improved, and the higher chondroblast differentiation capacity is shown.

A placental blood extractor consisting of an outside box having a plurality of plastic bags mounted within the top and bottom sections of the box and having an opening in the bottom section, a sterile bag having a central closure on a first side and a leader for an umbilical cord on a second side for holding a placenta mounted between the top and bottom plastic bags and a collector of blood system attached to the opening in the bottom, the collector of blood system consists of a pump, a manometer, a computer and a vibrator for gentle shaking of the collector of blood system.

The invention relates to a preparation method of a biology preparation, and specifically relates to a preparation method of placenta polypeptide for injection. The method takes use of placental blood in placenta of full term delivery of healthy parturient, and comprises the steps of inactivating bacteria, separating by centrifugation, clarifying and filtering, carrying out ultra filtration, sterilizing and filtering and freeze-drying. A process is simplified; production efficiency is increased; component differences among bathes of products are reduced; and quality indexes such as product activity are increased.

Methods, computer systems, and computer program products for maintaining a stem cell registry comprising information about a plurality of stem cell units. A donor is enrolled in the stem cell registry. A stem cell unit from the donor is characterized. Information about the stem cell unit, obtained by the characterizing, is recorded in the stem cell registry. Computer readable media comprising a plurality of data records. One or more respective data records in the plurality of data records comprises (i) a collection identifier number that uniquely corresponds to a stem cell donation, (ii) a cord blood cell count associated with the stem cell donation, and (iii) a placenta blood cell count associated with the stem cell donation. Additional computer readable media comprising a plurality of data records. One or more respective data records in the plurality of data records comprises (i) a cord blood cell count associated with a stem cell donation, (ii) a placenta blood cell count associated with the stem cell donation, and (iii) an indication of at least two stem cell transplant units in the stem cell donation.

A placental blood extraction device comprises a receptacle for receiving a placenta with umbilical cord, the receptacle comprising a compartment arranged to receive the placenta; and a tube extending from the compartment for receiving the umbilical cord; wherein the receptacle is selectively removable from the device for receiving the placenta. The placental blood extraction device may comprise a pressure application device for applying pressure to a placenta, the pressure application device comprising a plurality of pressing members for applying pressure on a plurality of regions of the placenta.

The invention relates to a culture method of peripheral blood CTL cells. The method comprises the following steps: 1) inoculating PBMC obtained by separating from placental blood to a culture vessel coated with a CD3 antibody and a CD28 antibody for culture, wherein the cell factor in the culture system is IFN-gamma 900U / mL-1100U / mL; 2) adding IL-1alpha and IL-2 into the culture system, with the final concentrations being both 900U / mL-1100U / mL, and also adding IL-7 into the culture system, with the final concentration being 4-6ng / mL; and 3) changing the solution, wherein cell factors in the new culture system are IL-2 900U / mL-1100U / mL, IL-7, 2-3ng / mL, and the basal culture medium is a VIVO culture medium containing 4-6% autologous plasma. The method has the advantages that the CTL amplification effect is good, the activated CTL proportion is relatively high, and the T-reg cell proportion is relatively low.

The invention relates to a method for separating and purifying placental blood by using a protective fluid and a placenta squeezer and a method for resuscitating the placental blood, and relates to the method for separating and purifying the placental blood by using the protective fluid and the placenta squeezer and the method for resuscitating the placental blood. The purpose of the invention isto solve the problems of difficult separation and extraction of placental blood, low viability of nucleated cells, low number of cells, easy contamination during preparation, and reduced cell qualityduring resuscitation in a conventional method. The placenta squeezer provided by the invention is used, and the protective fluid is added, so that the blood in a placenta is fully squeezed out, and through the efficient separation and purification method, the purposes that the total number of the nucleated cells can reach 15x10<8> or more, the viability is 95% or more, the colony formation is obvious, the phenotype flow detection CD34 reaches 0.3% or more, the pollution rate is controlled within 1%, the viability after resuscitation is 90% or more, the cloning ability is realized, the phenotype flow detection CD34 can reach 0.3% or more, and the sterility test is negative can be ensured. The methods are applied to the field of placental hematopoietic stem cell preparation.

The invention relates to the field of cell culture, in particular to a culture method of placental blood NK (natural killer) cells. The method comprises the steps as follows: inoculating a culture vessel coated with a CD16 monoclonal antibody with PBMC cells separated from placental blood, and performing culture, wherein components including FBS, IL-2, IL-12 and IL-15 are added to a culture systemon the basis of a VIVO-15 base culture medium; performing enlarged culture after 4-4.5 days of culture, wherein components including FBS, IL-2, IL-12 and IL-15 are added to a culture system for enlarged culture on the basis of a GT-T581 base culture medium. The culture method of the placental blood NK cells is easy to operate and low in cost, more NK cells meeting the clinical use standard can becultured, and a good foundation is laid for application of the placental blood NK cells.

The invention relates to a medical auxiliary appliance, especially a disposable pad for obstetric surgery, which includes a base plate; two drawstrings are fixedly arranged on one side of the baseplate, and both sides of the drawstring protrude outward to form a drawstring head that can be pulled by hand; the baseplate Two elastic bodies for adjusting the height and inclination angle of the base plate are also fixedly arranged on the same side; Advantages: The structure is simple and practical, it can effectively relieve the pressure of the uterus on the inferior vena cava, improve the blood circulation supply of mother and baby, and reduce the risk of placental insufficiency, supine hypotension and other complications; the length can be adjusted according to different puerpera The shape of the body is made into different lengths, which can facilitate the medical staff to carry gynecological patients under special circumstances, so that the patient will not cause secondary injuries when moving; in addition, the pull belt can also provide pregnant women with tools for grasping force during delivery, and absorbent pads It can effectively absorb amniotic fluid or blood and other dirt left by the lower body, so as not to pollute the surrounding environment.

The invention discloses a method for culturing placental blood hematopoietic stem cells. The method comprises the following steps that (1) placental blood is collected, diluted by 0.3-1.0 wt% of a sodium chloride solution, precipitated by 0.3-1.0 wt% of a methylcellulose solution, re-suspended by human AB plasma with concentration of 12-16% and separated and purified by a separation solution to obtain mononuclear cells; (2) a CD34<+> antibody is added to a mononuclear cell resuspension solution for incubation, then CD34<+> immunomagnetic beads are added for incubation, washing is performed with a new washing solution, and the mononuclear cell resuspension solution is placed in a magnetic field and subjected to standing to obtain CD34<+> cells; and (3) the CD34<+> cells are inoculated on aplacental blood hematopoietic stem cell culture medium for culture to obtain the placental blood hematopoietic stem cells. The culture method is stable and effective, the hematopoietic stem cells canproliferate for a long time without differentiation, the problem that cell apoptosis is promoted due to the fact that the hematopoietic stem cells secrete a large number of oxidation products in the process of proliferation is solved, and the amplification rate and cell activity of the hematopoietic stem cells are improved.

A placental blood extractor consists of an outside box having plurality of plastic bags for compressing the placenta and mounted to the top of the box. The lower part of the box has possibility to use several different circularly movable plates for mechanically facilitating the flow of the blood in the collecting veins on the fetal side of the placentas toward the central main umbilical cord vein. The box has an opening in the central lower part of the box and the lower area mechanical tray for the umbilical cord to exit the box. Blood is collected from umbilical cord and is to be collected into commercial collection bags. The area of the bag for collection of blood may be under negative pressure to facilitate the collection of blood but also gravity force may be used for that purpose. Both, the first part with the compression chambers as well as the second part, the Collector of Blood have pumps and manometers controlled by computer in their operations.

The invention provides a method for amplifying natural killer cells from placenta tissues, which comprises the following steps: (1) taking perinatal placenta tissues, and cutting down villus lobule structure tissues in a small block form; (2) digesting placental lobule tissues by utilizing I and II type collagenase, DNA enzyme and thermolysin; (3) extracting mononuclear cells in the digested cell suspension and mononuclear cells in placenta blood by using a full-automatic separation device method; (4) inducing amplification of the NK cells in the mononuclear cells by utilizing cell factors such as IL-15, FLT3-L and OK432; and (5) carrying out biological identification on the amplified NK cells. The method disclosed by the invention has excellent technical effects as described in the specification.

A placental blood extraction device comprises a receptacle for receiving a placenta with umbilical cord, the receptacle comprising a compartment arranged to receive the placenta; and a tube extending from the compartment for receiving the umbilical cord; wherein the receptacle is selectively removable from the device for receiving the placenta. The placental blood extraction device may comprise a pressure application device for applying pressure to a placenta, the pressure application device comprising a plurality of pressing members for applying pressure on a plurality of regions of the placenta.

The invention provides a method for preparing CD3+CD56+cells through high-killing K562 cells from perinatal placental blood. The method comprises the step of: separating mononuclear cells from the perinatal placental blood so as to carry out induction differentiation to form CD3+CD56+cells. By utilizing the method, the preparation period of the cells can be shortened, and moreover, the multiplication capability of the cells is improved.

The present invention relates to a solution for the preservation of cells from placental blood, from bone marrow and from peripheral blood

The present invention relates to multipotent adult stem cells derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart, and a method for preparing the same as well as a cellular therapeutic agent containing the same, more specifically, to a multipotent adult stem cell isolated by culturing an eukaryotic cell derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart in a FBS-containing medium and a method for preparing the same. Adult stem cells according to the present invention are derived from canine umbilical cord blood, placental blood and blood sample from canine fetal heart. The adult stem cells have characteristics highly similar to human mesenchymal stem cells as well as remarkable cell growth at the initial step compared to human UCB-derived mesenchymal stem cells so that the cells are useful to treat canine incurable diseases and difficult-to-cure diseases. Furthermore, multipotent adult stem cells are effective to treat musculoskeletal diseases and neural diseases due to the ability to differentiate into osteogenic cells and neural cells.

The invention discloses a method and device for measuring placental blood flow by utilizing flow-velocity compensated and non-compensated diffusion magnetic resonance. The method includes the following steps: based on a spin echo diffusion-weighted sequence, constructing a flow-velocity compensated (FC) diffusion-weighted sequence through bipolar gradient fields with same polarity; using the bipolar gradient fields with polar mirror symmetry to construct a non flow-velocity compensated (NC) diffusion-weighted sequence; respectively adopting the FC and NC sequences to measure the intra-voxel incoherent motion (IVIM) signals with multiple b values of placenta of pregnant women; and establishing an FC-NC joint model, and performing fitting to estimate the proportion and flow velocity of ballistic type blood microcirculation flow and the diffusion rate of tissue water. Compared with clinically and conventionally used IVIM imaging modes, the method can refine the ballistic type components and diffusion type components in microcirculation blood flow; and therefore, novel IVIM quantitative indexes can be provided, and the effects of the two kinds of microcirculation in placentas in a fetal-placental perfusion system can be quantitatively explained.

The invention discloses a high-purity allogeneic NK cell culture medium and an in vitro expansion method. Mononuclear cells were isolated from placental blood by lymphocyte separation medium, and the isolated mononuclear cells were added to a serum-free immune cell culture medium system containing CD314 antibody, IL-2, inactivated autologous plasma, etc. for 3 days; 10% inactivated autologous plasma and 1000IU / mL IL-2 were added once on the 4th and 5th days, respectively. After 7 days of continuous culture, a large number of cells were seen to expand, and the culture was continued for 2 days, and supplementary factors were added as needed. The present invention does not need to pre-coat the culture flask, and does not need to use feeder layer cells. After 14-16 days of culture, the prepared NK cell CD3-CD16+ / CD56+ cells have an expression rate of more than 90%, and the in vitro antitumor activity is strong, which can solve the problem of current In the NK culture system and technology, there are problems of low purity and poor killing activity of NK cells.