The present invention discloses a cloned embryo treatment liquid, which comprises BIX-01294 and a PZM culture liquid, wherein the BIX-01294 concentration in the PZM culture liquid is 5-500 nM, and 500 mL of the PZM culture liquid comprise 3.156 of NaCl, 1.053 g of NaHCO3, 0.373 g of KCl, 0.024 g of KH2SO4, 0.049 g of MgSO4, 0.308 g of calcium lactate, 0.011 g of pyruvic acid, 0.073 g of L-glutamic acid, 0.273 g of hypotaurine, 10 mL of BME, 5 mL of MEM, 0.033 g of penicillin, 0.025 g of streptomycin, and the balance of Sigma water. According to the present invention, with the application of the cloned embryo treatment liquid using the technical scheme to perform the culture treatment on the cloned embryo, the gene expression of SUV39H1, SETdb1, DNMT1 or DNMT3a in the cell can be significantly reduced, and the H3K9me2 levels of 2cell and 4cell at the early embryo development stage can be significantly down-regulated.