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Application of Pulsatilla chinensis decoction in preparation of drugs for reducing TLR-4 gene expression in intestinal microvascular endothelial cells

A technology of TLR-4 and Pulsatilla decoction, applied in antibacterial drugs, pharmaceutical formulas, plant raw materials, etc., can solve problems such as septic shock, inflammation, and pig fever, and achieve remarkable results

Inactive Publication Date: 2020-04-14
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in some pig farms, when colibacillosis occurs, a large amount of antibiotics will be used to treat colibacillosis. Although the death of E. coli is caused, a large amount of endotoxin is released at the same time, resulting in a large amount of endotoxin in the air, land, and drinking water of the pig farm. endotoxin, causing severe diseases such as fever, inflammation, anorexia, and septic shock in the pigs of the entire pig farm[16]

Method used

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  • Application of Pulsatilla chinensis decoction in preparation of drugs for reducing TLR-4 gene expression in intestinal microvascular endothelial cells
  • Application of Pulsatilla chinensis decoction in preparation of drugs for reducing TLR-4 gene expression in intestinal microvascular endothelial cells
  • Application of Pulsatilla chinensis decoction in preparation of drugs for reducing TLR-4 gene expression in intestinal microvascular endothelial cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 15g Coptidis Rhizome, 10g Cortex Phellodendron, 20g Peel of Qin, and 20g of Pulsatilla chinensis, put the weighed Chinese herbal medicines in the medicine pot, add enough distilled water to soak for 30min, boil with strong fire (rapid fire) and change to slow fire (slow fire) to continue Decoct for 30 minutes, use four layers of gauze to filter while hot, and collect the liquid medicine. Add a sufficient amount of distilled water to the remaining medicine dregs in the tank, boil it with strong fire, change to slow fire and continue to decoct for 30 minutes, filter it while it is hot with new gauze, collect the medicine liquid, repeat the above steps once, and finally combine the filtrate obtained three times, The combined filtrate was suction-filtered with a suction filtration device to remove residual medicinal residues. Use a rotary evaporator to concentrate the water decoction of Pulsatillae decoction to 65mL, so that its concentration is 1g / mL;

[0034] Pour ...

Embodiment 2

[0036] 1 material

[0037] 1.1 Test animals

[0038] SD rats aged 1-3 days were purchased from Beijing Xinglong Breeding Farm.

[0039] 1.2 Main reagents

[0040]

[0041]

[0042] 1.3 Main instruments

[0043]

[0044] 1.4 Configuration of main reagents

[0045] (1) DMEM basal medium

[0046] DMEM dry powder 1 bag

[0047] NaHCO 3 3.7g

[0048] wxya 2 O 1.0L

[0049] After completely dissolved, use 1.0mol·L -1 NaOH and 1.0mol·L -1 Adjust the pH value to 7.2-7.4 with HCL, and set the volume to 1.0L. After filter sterilization, aliquot and store at 4°C for later use.

[0050] (2) D-Hank's liquid

[0051] D-Hank’s dry powder 1 bag

[0052] NaHCO 3 0.35g

[0053] wxya 2 O 1.0L

[0054] After completely dissolved, use 1.0mol·L -1 NaOH and 1.0mol·L -1 Adjust the pH value to 7.2-7.4 with HCL, and set the volume to 1.0L. After filter sterilization, aliquot and store at 4°C for later use.

[0055] (3) DMEM complete medium with 20% FBS

[0056]

[0...

Embodiment 3

[0101] Coptidis 15g, Phellodendron 10g, Qinpi 20g, Pulsatilla 20g;

[0102] Soak the raw material drug in water for 20-60 minutes, preferably 30 minutes, decoct for 30 minutes, collect the medicinal solution after filtration, add water to continue decocting for 30 minutes, filter, collect the medicinal solution, add water to continue decocting for 30 minutes, filter, and decoct the obtained 3 times The filtrates were combined, filtered and concentrated to a concentration of 1g / mL, dried in a drying oven at 80°C, and dried to obtain the liquid medicine.

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Abstract

The invention discloses application of a Pulsatilla chinensis decoction in drugs for reducing TLR-4 gene expression in intestinal microvascular endothelial cells. The Pulsatilla chinensis decoction comprises, by weight, 10-15 parts of Coptis chinensis, 5-15 parts of golden cypress, 15-30 parts of ash bark and 15-30 parts of Pulsatilla chinensis. The Pulsatilla chinensis decoction remarkably reduces the TLR-4 gene expression in the intestinal microvascular endothelial cells, and the effect is obvious.

Description

technical field [0001] The invention relates to a new application of Pulsatilla Decoction, in particular to the application of Pulsatilla Decoction in reducing the expression of TLR-4 protein in intestinal microvascular endothelial cells. Background technique [0002] Bacterial diseases lead to the death rate of food animals ranking first among all kinds of diseases. Over the years, in the prevention and treatment of bacterial diseases, antibiotics have been used too much, but problems such as drug residues and drug resistance caused by antibiotics have also followed. . Therefore, traditional Chinese veterinary drugs, which have the advantages of less residue, low toxicity, and less resistance to drug resistance, have become the first choice to replace antibiotics. Microvascular endothelial cells (MVECs) are the initial target cells of pathogenic factors, which can adapt and regulate the activities of organs and the whole body. [0003] Escherichia coli is divided into pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/756A61P31/04
CPCA61K36/63A61K36/71A61K36/718A61K36/756A61K2236/331A61K2236/39A61P31/04A61K2300/00
Inventor 董虹颜紫骐蒋林树刘晓晔穆祥王芸
Owner BEIJING UNIV OF AGRI
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