50 results about "Osseous Cell" patented technology

The invention provides a preparation method of a multifunctional layered joint cartilage support. The multifunctional layered joint cartilage support capable of simulating the biological property andthe mechanical property of natural cartilage is prepared by taking hydrogel as a support material, taking mesenchymal stem cells, cartilage cells and osteoblasts as inducing and developing objects andby adopting a 3D printing technology. The multifunctional layered joint cartilage support can accurately simulate the internal tissue form and the contour of each layer of the natural cartilage and can realize that different layers have different mechanical and biological characteristics. The prepared artificial cartilage support cells are derived from patients, the support serves as a degradablehydrogel material and the rejection reaction after implantation is greatly reduced. Various nutritional substances and growth factors are mixed in the hydrogel support, so that the sustained releaseeffect can be achieved and regulation and control of the growth and development of the artificial cartilage can be realized.

The invention provides a preparation method of a degradable medical macromolecular three-dimensional material for improving osteoblast adhesion and osteogenic property. The method comprises the following steps: compounding a degradable medical macromolecular material and an active substance capable of promoting osteoblast adhesion and proliferation, and preparing a film with a three-dimensional structure; carrying out surface functionalization treatment for the film with the three-dimensional structure; carrying out surface modification for the film with the three-dimensional structure after the surface functionalization by adopting the active substance with osteoblast adhesion and proliferation activity; and washing and drying the surface modified film with the three-dimensional structure to obtain a product. Compared with the prior art, the degradable medical macromolecular three-dimensional material for improving the osteoblast adhesion and osteogenic property prepared by virtue of the method can maintain the mechanical property and the structure and realize the combination of the bulk-phase low-concentration long-term expression and surface high-concentration short-time expression of the osteogenic active substance, and the adhesion and three-dimensional growth of the osteoblast can be obviously improved.

The invention discloses an antibacterial functional gradient porous HA-Ag bone filling scaffold with an active interlayer and belongs to the technical field of biomedical material preparation. The functional gradient porous HA-Ag bone filling scaffold material with the active interlayer is prepared by weighing and ball-milling hydroxyapatite HA and Ag powder according to an ingredient proportion, weighing and mixing mixed powder and an ammonium bicarbonate pore former according to a porosity proportion, preparing three kinds of powder, then laying the powder in a gradient manner, mechanically pressing into a block pressed blank, putting the pressed blank into a discharge plasma sintering furnace, performing sintering after vacuuming a system to 2-6Pa, heating at a rate of 50-100min/DEG C, keeping at 800-1000 DEG C for 5-10min, and cooling to room temperature in the furnace. The material can induce adhesion and proliferation of bone cells on the surface of the material actively, has good osteogenesis activity and antibacterial property, high porosity and mechanical stability at the same time, and can serve as a good artificial bone filling material.

The invention provides a culture method for inducing mesenchymal stem cell to differentiate for forming osteocyte under the condition without CO2. The culture method comprises the following steps of: culturing the third-generation human mesenchymal stem cell under the conditions of 5 percent of CO2 and 37 DEG C and standing overnight; after the cell is completely attached to the wall and grows until 80 percents of cell is fused, replacing an inductive culture medium and culturing under the conditions of 37 DEG C without CO2 for 14-21 days to obtain osteoblast, wherein the inductive culture medium is formed by adding fetal calf serum, penicillin, streptomycin, hexadecadrol, beta-sodium glycerophosphate and vitamin C in a basal medium and is prepared from the following components calculatedby final concentration: 0.41g/L of sodium bicarbonate, 1.5g/L of disodium hydrogen phosphate dodecahydrate, 0.07g/L of monopotassium phosphate and 8.17g/L of sodium chloride; and solvent of the inductive culture medium is an L-DMEM cell culture medium free of sodium bicarbonate. According to the novel culture medium formula provided by the invention, the mesenchymal stem cell osteoblast can be normally induced to differentiate; the differentiation effect is favorable, so that the consumption of equipment is reduced; and the novel culture medium formula is suitable for cell culture and research on induced differentiation under the condition without CO2.

The invention provides a bone repairing material, and a preparation method and application of the bone repairing material. The preparation method of the material comprises the steps: dissolving a structure directing agent and a pore-enlarging agent, adding a calcium source, a silicon source and a phosphorus source, adjusting a pH (potential of hydrogen) value to 9-11, placing for 56-80 h at 90-110 DEG C, baking for 6-10 h at 550-600 DEG C to form bioactivity glass, mixing the bioactivity glass and 3-aminopropyltriethoxysilane for 14-20 h, baking for 20-30 h at 80-150 DEG C, washing with chloroform, drying to form amino modified bioactivity glass, and incubating the amino modified bioactivity glass and a cell factor in a carbon dioxide incubator at 37 DEG C for 4-6 h to promote adsorption to form the bone repairing material. The material can promote osteoblast proliferation, osteogenesis direction differentiation and mineralization processes, has high cell compatibility and a good effect of promoting bone formation and has wide application prospects as the repairing material in a bone defect repairing process.

The invention discloses active peptides for promoting osteoblast proliferation and application thereof. The active peptides have amino acid sequences as shown in SEQ ID NO: 1 to SEQ ID NO: 5. The active peptides can promote osteoblast proliferation so as to prevent or treat osteoporosis. These active peptides can further be developed as pharmaceuticals, food or health products.

The invention encompasses compositions and methods for effectively interfering, reducing and preventing conversion of vascular smooth muscle cells (VSMCs) and circulating stem cells to osteoblastic bone-like cells, thereby reducing and/or preventing vascular calcification (VC) or calcium mineral (hydroxyapatite) deposition in the vasculature. The severity and extent of calcification in the major arteries reflect atherosclerotic plaque burden and strongly predict cardiovascular morbidity and mortality. The present inventive compositions used for administration to human and other mammalian subjects comprise select actives that inhibit, interfere or regulate the biochemical processes leading to such calcification and include (1) at least one agent that modulates expression and/or activity of peroxisome activated protein receptor gamma (PPAR-γ); (2) at least one agent that inhibits expression and/or suppresses activity of one or more of the osteogenic transcription factors (Cbfα1/Runx2, Osterix, Msx2) and β-catenin signaling; (3) at least one agent that inhibits expression and/or suppresses activity of one or more of bone morphogenetic proteins (BMPs: BMP 2 and 4), alkaline phosphatase (ALP), and osteocalcin; (4) at least one agent that inhibits the activity of Reactive Oxygen Species (ROS); and (5) at least one agent that suppresses one or more of inflammatory mediators including interleukins IL-1α, IL-1β, IL-6, NF-κB, TNF-α, matrix metalloproteinases (MMPs) and prostaglandin E2 (PGE2). The compositions may further comprise at least one agent that promotes expression and/or carboxylation of matrix Gla protein (MGP). Advantageously, these select actives include materials such as phytonutrients, vitamins and minerals that have been broadly used in food and drink products and are safe for human and pet/animal consumption. Compositions with such combinations have the ability to prevent, treat and reverse VC not only in coronary arteries but also in other tissues capable of undergoing undesirable calcification. In addition the present compositions are effective against associated conditions or contributory factors/inducers to VC, including diabetes, obesity, hypertension, inflammation, oxidative stress, osteoporosis and arthritis.

The invention discloses a small-molecule inhibitor for a bone formation negative regulatory factor Smurf1. The compound shown as a formula I can be used in preparation of medicines for promoting bone formation and preparation of a BMP sensitizing agent. The invention also provides one of the applications of the compound shown as the formula I: (1) preparation of products for improving osteogenic potential of cells, wherein the cells are specifically myogenic precursor cells or osteogenic precursor cells; (2) preparation of products for improving alkaline phosphatase, osteocalcin and / or type I collagen mRNA levels in cells, wherein the cells are specifically myogenic precursor cells or osteogenic precursor cells; (3) preparation of products for improving activity of alkaline phosphatase in myogenic precursor cells or osteogenic precursor cells or osteoblasts; (4) preparation of products for inhibiting degradation of an Smad1 / 5 protein under the condition of BMP pathway activation; and (5) preparation of products for reducing ubiquitination level of the Smad1 / 5 protein under the condition of BMP pathway activation.

The invention relates to a capsule for increasing bone density and a preparation method of the capsules. The capsule comprises a capsule body and filler filling the capsule body, wherein the filler is prepared from the following raw materials in parts by weight: 25 parts of extracts of fortune's drynaria rhizome, 25 parts of eucommia bark extracts, 80 parts of milkvetch root extracts, 70 parts of Kudzuvine root extracts, 180 parts of oyster extracts, 18 parts of starch and 2 parts of magnesium stearate. The capsule strengthens the activity of osteoblasts, promotes production and secretion of bone matrixes and bone mineralization and improves the body immune function; the components are applied compatibly, realize a synergistic effect, improve calcium absorption of human bodies together and increase the bone density; the preparation method is reasonable in process and high in operability and easily realizes large-scale production.

The invention discloses a polylactic acid/hydroxyapatite/acellular amniotic membrane composite stent and a construction method thereof. The polylactic acid/hydroxyapatite/acellular amniotic membrane composite stent is characterized in that polylactic acid, hydroxyapatite/acellular and amniotic membrane are dissolved into dichloroethane and n-hexane by steps, and a non-solvent induced phase separation method is adopted to construct the composite stent which is of a composite pore structure using large pores to coat small pores. The polylactic acid/hydroxyapatite/acellular amniotic membrane composite stent has the advantages that by using the polylactic acid as a substrate, the mechanical strength and biodegradability of the stent are guaranteed; by compounding with the hydroxyapatite, whilethe bone inducing property and bone conduction property of the stent are improved, the acidifying of the metabolism environment is relieved; by compounding with the acellular amniotic membrane, the water-absorbing property, biocompatibility and identification property of the stent are improved, and the sticking and propagation of cells on the stent are promoted; the porosity is high, the pore communication property is good, the mechanical property is strong, the biocompatibility is good, the osteogenesis inducing activity is high, and the like; the good biology function on important seed cells and osteogenesis cells in bone tissue engineering is realized, and the polylactic acid/hydroxyapatite/acellular amniotic membrane composite stent can be used as an excellent biomedical material to be applied to the field of bone tissue engineering.

The invention discloses a regulation and control research method of cell cilium transportprotein 80 in rapid maxillary expansion mechanical biological signal transduction. The method comprises the following steps: a, establishing a rapid maxillary expansion mouse animal model; b, establishing IFT80 gene silence osteoblast; c, establishing an in-vitro stretching culture osteoblast model; d, establishing a co-culture model of osteoblast and osteoclast; e, carrying out in-vivo transfection experiment; f, confirming a relationship of IFT80 spatio-temporal expression and rapid maxillary expansion midline palatine suture bone remolding; g, confirming regulation and control mechanisms of IFT80 upon proliferation and differentiation of in-vitro stretching cultured osteoblast, primary cilium formation and mechanical biological signal transduction; h, confirming influence of IFT80 gene silence upon in-vitro stretching cultured osteoblast in regulating functions of osteoclast. The method has great instruction significances for treating such malocclusion.