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Application of APS (astragalus polysaccharide) to prevention of BMSCs (bone marrow mesenchymal stem cells) injury caused by radiation A549 cell bystander effect

An astragalus polysaccharide and cell damage technology, which is applied in the application field of astragalus polysaccharide in the protection of radiation A549 cells to produce side effects on BMSCs damage

Pending Publication Date: 2018-01-19
GANSU UNIV OF CHINESE MEDICINE
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Problems solved by technology

Therefore, at present, only the killing of tumor cells by radiation has been paid attention to clinically, and little attention has been paid to the damage and safety issues of MSCs caused by radiation therapy for lung cancer.

Method used

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  • Application of APS (astragalus polysaccharide) to prevention of BMSCs (bone marrow mesenchymal stem cells) injury caused by radiation A549 cell bystander effect
  • Application of APS (astragalus polysaccharide) to prevention of BMSCs (bone marrow mesenchymal stem cells) injury caused by radiation A549 cell bystander effect
  • Application of APS (astragalus polysaccharide) to prevention of BMSCs (bone marrow mesenchymal stem cells) injury caused by radiation A549 cell bystander effect

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Embodiment 1

[0022] 1. Materials and Instruments

[0023] 1.1 Cell lines

[0024] Human bone marrow mesenchymal stem cell line: human bone marrow mesenchymal stem cells were purchased from Guangzhou Saiye Biotechnology Co., Ltd. (batch number: 7500, the identification certificate is attached).

[0025] Human A549 cell line was purchased from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (CatalogNumber: TCHu150).

[0026] 1.2 Main reagents

[0027] MSCM special medium, Guangzhou Saiye Biotechnology Co., Ltd., batch number: 7500;

[0028] Penicillin-streptomycin, Guangzhou Saiye Biotechnology Co., Ltd., batch number: 7500;

[0029] FBS, Hyclone, lot number: SV30087.02;

[0030] Bone marrow mesenchymal stem cell osteogenic differentiation medium kit, Guangzhou Saiye Biotechnology Co., Ltd., batch number: HUXMA-90021;

[0031] Dimethyl sulfoxide (DMSO), American Sigma company, lot number: BCBH7614V;

[0032] DMEM / F-121:1 (1X) medium,...

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Abstract

The invention relates to application of APS (astragalus polysaccharide) to prevention of BMSCs (bone marrow mesenchymal stem cells) injury caused by the radiation A549 cell bystander effect. After theAPS interference is performed in advance, the influence on the BMSCs by radiation and radiation bystander injury can be obviously reduced, so that the quantity of cells differentiated into nerve cells is increased; the length of synapses differentiated into the nerve cells is increased; the expression of Nissl substances and neuro-specific marker proteins NSE and Nestin is increased; meanwhile, the osteoblast cell differentiation calcium nodule area and the osteoblast cell differentiation mature labelled proteins OPN and OCN are increased, so that the protection effect is achieved on the influence of the BMSCs differentiation potential caused by radiation and radiation bystander injury can be realized by the APS.

Description

technical field [0001] The invention relates to a new application of astragalus polysaccharide, and more specifically relates to the application of astragalus polysaccharide in protecting BMSCs from the side effect of radiation A549 cells. Background technique [0002] Lung cancer is one of the malignant tumors with the highest morbidity and mortality in the world. In my country, lung cancer is also the main cause of cancer death, and more than 80% of all lung cancers are non-small cell lung cancers. At present, chemotherapy, radiotherapy and surgery are the main treatment methods for lung cancer. The side effects of chemotherapy are serious, and surgical treatment is easy to relapse. Therefore, radiotherapy is an effective means for local treatment of malignant tumors. Commonly used clinical radiotherapy methods include X-rays, gamma rays, electron beams, neutron beams, and proton beams. X-rays should have the characteristics of short wavelength, high frequency, and strong...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/079C12N5/077
Inventor 刘永琦张利英卢志伟周妮娜王磊张苡铭张丽昕许小敏
Owner GANSU UNIV OF CHINESE MEDICINE
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