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Two-layer mimetic periosteum material with HAp loaded inner layer and VEGF loaded outer layer,

A technology of inner and outer layers and membrane materials, used in medical science, prosthesis, tissue regeneration, etc., can solve the problem of difficult bone repair materials, achieve synergistic effect of promoting vascularization, facilitate adhesion and growth, and promote the induction of bone cell growth. Effect

Inactive Publication Date: 2018-02-16
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But how to apply it to bone repair materials is a difficult problem

Method used

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  • Two-layer mimetic periosteum material with HAp loaded inner layer and VEGF loaded outer layer,
  • Two-layer mimetic periosteum material with HAp loaded inner layer and VEGF loaded outer layer,
  • Two-layer mimetic periosteum material with HAp loaded inner layer and VEGF loaded outer layer,

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1L of Ca(NO with a concentration of 0.5mol / L 3 ) 2 .4H 2 O aqueous solution was added to a 5L three-neck flask, and the pH of the solution was adjusted to ~10 by adding concentrated ammonia water (concentration 25%~28%) to it. Take two single-necked pear-shaped flasks, add 600mL, 0.5mol / L (NH 4 ) 2 HPO 4 Aqueous solution, 800 mL dilute ammonia solution (composed of concentrated ammonia water and deionized water with a volume ratio of 1:9) was added to another flask. The (NH 4 ) 2 HPO 4 The aqueous solution and dilute ammonia solution were added dropwise at a flow rate of 5 mL / min to the Ca(NO 3 ) 2 .4H 2 O aqueous solution in a three-necked flask, while mechanically stirring at a speed of 600 rpm. Wait (NH 4 ) 2 HPO 4 After the aqueous solution was added dropwise, the resulting mixture was poured into a beaker, left to age at room temperature for 24 hours, and then the supernatant was poured off. Then, 1.5 L of absolute ethanol was added to the beaker, sti...

Embodiment 2

[0036] The preparation of HAp powder is shown in Example 1.

[0037] Dissolve 0.160 g HAp, 0.231 g SF and 0.464 g P(DLLA-co-CL) in 8 g HFIP, stir at room temperature for 12 h and then sonicate for 20 min to obtain a homogeneous solution, which is added to a 10 mL syringe HAp / SF-P(DLLA-co-CL) fiber membrane with HAp loaded on the inner layer was obtained by electrospinning equipment under the conditions of 13 KV spinning voltage, 13 cm receiving distance, and 0.5 mL / h solution flow rate.

[0038] When the above solution was spun, the pre-prepared HFIP solution dissolved with VEGF and SF (0.64 g SF and 32 mg VEGF dissolved in 8 g HFIP) was added into another 10 mL syringe and passed through the electrospinning device at 15 The spinning voltage of KV, the receiving distance of 15 cm, and the solution flow rate of 0.5 mL / h continued to spin a layer of VEGF / SF fiber on the surface of the HAp / SF-P(DLLA-co-CL) fiber membrane prepared above. Membrane, the final HAp / SF-P(DLLA-co-CL)@V...

Embodiment 3

[0042] The preparation of HAp powder is shown in Example 1.

[0043] Dissolve 0.128 g HAp, 0.64 g SF and 0 g P(DLLA-co-CL) in 8 g HFIP, stir at room temperature for 12 h and then sonicate for 20 min to obtain a homogeneous solution, which is added to a 10 mL syringe HAp / SF fiber membrane with HAp loaded on the inner layer was obtained by electrospinning equipment under the conditions of 15 KV spinning voltage, 17 cm receiving distance, and 0.5 mL / h solution flow rate.

[0044] When the above solution was spun, the pre-prepared HFIP solution dissolved with VEGF and SF (0.64 g SF and 66 mg VEGF dissolved in 8 g HFIP) was added into another 10 mL syringe and passed through the electrospinning device at 15 The spinning voltage of KV, the receiving distance of 15 cm, and the solution flow rate of 0.5 mL / h continued to spin a layer of VEGF / SF fiber on the surface of the HAp / SF-P(DLLA-co-CL) fiber membrane prepared above. Membrane, the final HAp / SF-P(DLLA-co-CL)@VEGF / SF bilayer fibe...

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Abstract

The present invention relates to a two-layer mimetic periosteum material with a HAp loaded inner layer and a VEGF loaded outer layer, a preparation method and applications. According to the present invention, silk fibroin (SF) and poly(racemic lactic acid-co-caprolactone) (P(DLLA-co-CL)) are used as base materials, the fibrous porous two-layer mimetic periosteum material is constructed through anelectrospinning technology, the inner layer can promote the bone regeneration by doping HAp, and the outer layer can promote the vascularization by doping VEGF; the two-layer mimetic periosteum material has the fibrous porous structure similar to the autologous bone growth microenvironment, such that the adhesion and the growth of osteoblasts can be easily achieved; and by respectively loading HApand VEGF on the inner layer and the outer layer, the two-layer mimetic periosteum material induced osteoblast growth and vascularization synergy can be effectively promoted, such that the important theoretical research significance and practical value can be provided for the rapid repair of bone tissues.

Description

technical field [0001] The invention relates to a double-layer periosteum-imitative material, in particular to a double-layer periosteum-imitative material whose inner and outer layers are respectively loaded with HAp and VEGF, as well as its preparation method and application. Background technique [0002] Bone defect is a common clinical disease, and its clinical repair methods mainly include autologous bone graft, allogeneic bone graft and artificial bone repair. Among them, the artificial bone grafting method has the disadvantages of small bone fragments, limited bone sources, and new wounds for patients. Allogeneic bone grafting often has the risks of immune rejection, inflammation, and infection. Compared with these two methods, the artificial bone repair method avoids the limitations of the former two by developing an artificial bone repair material whose performance is similar to that of autogenous bone without relying on autogenous bone, which has great significanc...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/46A61L27/48A61L27/56
CPCA61L27/46A61L27/48A61L27/56A61L2300/112A61L2300/412A61L2300/414A61L2430/02C08L89/00C08L67/04
Inventor 何丹农朱海娥叶恺王萍金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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