294 results about "Fibroblastic cell" patented technology

Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.

The invention provides improved methods for producing induced pluripotent stem cells (iPSC) from adult fibroblasts. The methods include contacting adult fibroblasts with a reprogramming composition suitable for reprogramming the adult fibroblasts to iPSC, under conditions effective for the reprogramming composition to penetrate the adult fibroblasts, followed by culturing the contacted fibroblasts for a time period sufficient for the cells to be reprogrammed. The cultured cells are then sorted to select cells based upon their expression of the cell membrane surface markers CD13NEG SSEA4POS Tra-1-60POS. iPSC colonies are then identified from the sorted cells.

The present invention provides a novel method to isolate and expand pure progenitor / stem cells from a primary tissue explant, which produces a population enriched in multipotent functional progenitor / stem cells free of contaminating fibroblasts and other cell types. Cardiac progenitor / stem cells isolated by this method maintain their self-renewal and clonogenic character in vitro and differentiate into normal cells in myocardium, including cardiomyocytes, endothelial cells, and smooth muscle cells, after transplantation into ischemic hearts. The present invention also includes substantially pure populations of multipotent progenitor / stem cells, e.g., cardiac progenitor / stem cells, and their use to treat and prevent diseases and injuries, including those resulting from myocardial infarction.

The invention relates to bioreactors having an enclosed chamber, a sheet growth module, a rollable mandrel, and a clamp for holding the sheet to the mandrel for rolling for the manufacture of a tissue engineered blood vessel (TEBV). The TEBV is made from a cultured fibroblast sheet rolled into a multilayer vessel which has sufficient burst strength to withstand physiological blood pressure without the inclusion of smooth muscle cells or synthetic scaffolding.

The present disclosure provides method of generating cardiomyocytes from post-natal fibroblasts. The present disclosure further provides cells and compositions for use in generating cardiomyocytes.

The invention relates to a centella asiatica triterpenic acid single-glucopyranoside composition which is composed of ursane madecassic acid single-glucopyranoside, madecassic acid single-glucopyranoside and oleanane chebuloside II, wherein the mass ratio of ursane madecassic acid single-glucopyranoside to madecassic acid single-glucopyranoside to oleanane chebuloside II is 1:0.5-2:0.1-1, and the sum of the mass percentage content of three components is not less than 50%. The composition takes a centella asiatica extract as a substrate, the centella asiatica extract is fermented and hydrolyzed by microbes of beta-glucosidase or microbes capable of generating beta-glucosidase, and extracted by n-butanol or separated and purified by macroporous adsorption resin. The invention also provides a quantitative analysis method which is a HPLC quantitative analysis for three components by adding a proper amount of mobile phase of beta-cyclodextrin. Experimental research of pharmacodynamics proves that the composition has substantial activity for inhibiting tumor cells and fibroblast, the composition can be used for treating tumor and scar hyperplasia.

A transcutaneous prosthesis includes a first component configured for attachment to a bone, the first component including flutes or grooves on a surface thereof for deterring rotation of the prosthesis within a bone; a second component adapted for location between the bone and the skin, the second component having a surface treatment for stimulation of fibroblastic cell proliferation and attachment of epithelial cells; and a third component adapted for location to extend from the skin surface and is adapted to extend directly from the skin surface in use, the third component having a coating of a non-stick material on an outer surface thereof, the coating having a surface energy that is lower than a surface energy of the first and second components and which is low enough to deter bacterial adhesion.

Three-dimensional tissue-like organization of cells by high cell-seeding-density culture termed as macromass culture is described. By macromass culture, cells can be made to organize themselves into a tissue-like form without the aid of a scaffold and three-dimensional macroscopic tissue-like constructs can be made wholly from cells. Tissue-like organization and macroscopic tissue-like constructs can be generated from fibroblastic cells of mesenchymal origin (at least), which can be either differentiated cells or multipotent adult stem cells. In this work, tissue-like organization and macroscopic tissue-like constructs have been generated from dermal fibroblasts, adipose stromal cells-derived osteogenic cells, chondrocytes, and from osteoblasts. The factor causing macroscopic tissue formation is large scale culture at high cell seeding density per unit area or three-dimensional space, that is, macromass culture done on a large scale. No scaffold or extraneous matrix is used for tissue generation, the tissues are of completely cellular origin. No other agents (except high cell-seeding-density) that aid in tissue formation such as tissue-inducing chemicals, tissue-inducing growth factors, substratum with special properties, rotational culture, etc, are employed for tissue formation. These tissue-like masses have the potential for use as tissue replacements in the human body. Tissue-like organization by high cell-seeding-density macromass culture can also be generated at the microscopic level.

The invention discloses a multilayer core-shell structured nano-fiber scaffold, and a method for constructing a tissue engineering material by using the multilayer core-shell structured nano-fiber scaffold and melanocyte. The multilayer core-shell nano-fiber scaffold is prepared through electrostatic spinning, the external layer of the scaffold is a biocompatible material, the middle layer of the scaffold is a polymer barrier layer, and the core layer of the scaffold is a drug supported core material. The tissue engineering material can be formed by using the multilayer nano-fiber scaffold and the melanocyte, or using the multilayer nano-fiber scaffold, fibroblast and keratinocyte, and can be used to treat depigmentation (such as leucoderma). The multilayer core-shell structured nano-fiber scaffold has the advantages of good biocompatibility and mechanical performances, and realization of long-time controllable release of drugs, and the tissue engineering scaffold constructed by using the multilayer core-shell structured nano-fiber scaffold can effectively support growth, propagation and transplantation of melanocyte and co-culture cells.

A synthetic tetrapeptide having an amino acide sequence, Glycine-Proline-Arginine-Proline (GPRP) has pro-inflammatory effects on human fibroblastic cells, including synovial cells. An amide analog of GPRP is ineffective in inducing, or effective in causing a loss of, pro-inflammatory effects.

Methods, devices, kits and compositions to treat a myocardial infarction. In one embodiment, the method includes the prevention of remodeling of the infarct zone of the ventricle using a combination of therapies. The method may include the introduction of structurally reinforcing agents. In other embodiments, agents may be introduced into a ventricle to increase compliance of the ventricle. The prevention of remodeling may include the prevention of thinning of the ventricular infarct zone. Another embodiment includes the reversing or prevention of ventricular remodeling with electro-stimulatory therapy. The unloading of the stressed myocardium over time effects reversal of undesirable ventricular remodeling. These therapies may be combined with structurally reinforcing therapies. In other embodiments, the structurally reinforcing component may be accompanied by other therapeutic agents. These agents may include but are not limited to pro-fibroblastic and angiogenic agents.

A three-dimensional reconstruct model containing solid layers of collagen and fibroblasts closely mimic the condition of patients in vivo, preserving in vivo phenotypic and functional characteristics of the cells. Reconstructs can be used to detect cellular migration, including active migration of cytotoxic T lymphocytes towards tumor cells, which can be measured by detecting tumor cell lysis. Reconstructs also can be used to identify factors that influence migration of cells, including chemokines that influence migration of cytotoxic T lymphocytes, as well as to identify tumor antigens

The present invention discloses a melanin-containing skin models in vitro skin test model and a preparation method and use. The test model comprises a dermis layer and an epidermis layer, the epidermis layer comprises epidermal cells and melanin cells, and the dermis layer is a membrane comprising fibroblast and an extracellular matrix formed by the fibroblast. The skin model is very similar to the human skin structure, the building process simulates the skin development process, the epidermis layer can be developed under nourishing of the dermis layer, and synthesis, secretion, transport and the like of melanin can be promoted. The present invention discloses a preparation method and use of the skin test model in whitening and freckle removal effect testing of a variety of cosmetics, health products and pharmaceuticals, related mechanism exploration and research, and the like.

The invention discloses a core-shell type nano fiber bracket and a method for constructing a tissue engineering material by the core-shell type nano fiber bracket and melanocytes. A core-shell type nano fiber is prepared through electrostatic spinning; a shell layer is a biocompatible material and a core layer is in-situ cross-linked polymer hydrogel. The tissue engineering material is constructed by using the nano fiber bracket and the melanocytes or the nano fiber bracket, mechanocytes and keratinocytes, and can be used for treating depigmentation (such as leucoderma). The core-shell type nano fiber bracket disclosed by the invention has good biocompatibility and biodegradability, and can be used for loading medicines and realizing controllable releasing of the medicines; a tissue engineering bracket constructed by the core-shell type nano fiber bracket can be used for effectively supporting the growth, multiplication and transplanting of the melanocytes and co-culture cells.

The invention provides a method for simultaneously preparing autologous epidermal cells and fibroblasts. The method comprises the steps of obtaining the epidermal cells from autologous skin, and culturing the epidermal cells to obtain the fibroblasts. The invention further provides a biological beauty product comprising the autologous epidermal cells and / or the fibroblasts. One or two cells are added into autologous peripheral blood platelet-rich plasma, and meanwhile, nutritional factors can be added to prepare the biological beauty product comprising autologous living cells; the product has beautifying effects of removing vitiligo macules, repairing scars, removing skin wrinkles and spots, and the like.