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Bushi lactobacillus prepn., prepn. method and use thereof

A technology of Lactobacillus Brucella preparations, which is applied in Lactobacillus Brucella preparations to improve the stability of whole-plant corn silage after opening. Its preparation, functional strains - Lactobacillus Brucella preparations, can solve the risk, corn silage Feed quality decline, loss and other issues to achieve the effect of improving stability, resolving feed safety risks, and improving quality

Inactive Publication Date: 2006-09-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lactic acid fermentation of corn silage prepared by traditional methods, the concentration of acetic acid and propionic acid is low after the silage is completed. After the cellar is opened, due to the destruction of the anaerobic environment, a large number of gas-consuming microorganisms re-grow, causing its stability to sharply decrease. decline, the main features are yeast and mold overgrowth, accelerated oxidation of glucose, and CO 2 The rapid increase in production not only causes the quality of corn silage to decline, but also brings a great risk to feed safety due to the mycotoxins produced by a large number of molds
If it is not used in time or the speed of use is too slow, it will cause a lot of losses due to the sharp decline in its stability

Method used

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  • Bushi lactobacillus prepn., prepn. method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The preparation of Lactobacillus buchneri is fermented and cultured by Lactobacillus buchneri (L.buchneri) and freeze-dried; each gram contains 9.5×10 10 L. buchneri colony forming unit (cfu / g).

[0063] Among them, Lactobacillus brauchneri was purchased from the China Center for Culture Collection of Microorganisms (CICC), and the CICC number is 6007.

[0064] The preparation of Lactobacillus brunii contains 33% freeze-drying protectant, and the freeze-drying protectant is glycerin and skimmed milk, and the volume ratio is 1:1.

[0065] Concrete preparation process is as follows:

[0066] 1. Activation culture of Lactobacillus Brucella

[0067] In the ultra-clean workbench, burn the inoculation loop at the inner flame of the alcohol lamp, inoculate the Lactobacillus Brucella powder into the MRS liquid medium, burn the test tube mouth and rubber stopper, and plug the test tube. Put the Lactobacillus Brucella into a shaker at 30°C for 18 hours.

[0068] MRS liquid me...

Embodiment 2

[0079] Lactobacillus buchneri preparations are fermented and cultured by Lactobacillus buchneri (L.buchneri) and freeze-dried; each gram contains 1.2×10 10 L. buchneri colony forming unit (cfu / g).

[0080] It contains 40% lyoprotectant, the lyoprotectant is glycerin and skimmed milk, and the volume ratio is 1:2.

[0081] Concrete preparation process is as follows:

[0082] 1. Activation culture of Lactobacillus Brucella

[0083] In the ultra-clean workbench, burn the inoculation loop at the inner flame of the alcohol lamp, inoculate the Lactobacillus Brucella powder into the MRS liquid medium, burn the test tube mouth and rubber stopper, and plug the test tube. Put the Lactobacillus Brucella into a shaker at 32°C for 15 hours.

[0084] MRS liquid medium, its composition is: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween-801ml, ammonium citrate 2g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, formazan hydrochloride (MgSO 4 ·7H 2 O 11.5g+MnSO ...

Embodiment 3

[0095] Lactobacillus buchneri preparations are fermented and cultured by Lactobacillus buchneri (L.buchneri) and freeze-dried; each gram contains 1.0×10 11 L. buchneri colony forming unit (cfu / g).

[0096] It contains 30% lyoprotectant, the lyoprotectant is glycerin and skimmed milk, and the volume ratio is 1:1.

[0097] Concrete preparation process is as follows:

[0098] 1. Activation culture of Lactobacillus Brucella

[0099] In the ultra-quiet workbench, burn the inoculation loop at the inner flame of the alcohol lamp, inoculate the Lactobacillus Brucella powder into the MRS liquid medium, burn the test tube mouth and rubber stopper, and plug the test tube. Put Lactobacillus brucei on a shaker at 28°C for 20 hours.

[0100] MRS liquid medium, its composition is: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween-801ml, ammonium citrate 2g, sodium acetate 5g, dipotassium hydrogen phosphate 2g, formazan hydrochloride (MgSO 4 ·7H 2 O 11.5g+MnSO 4 4H 2 ...

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Abstract

The invention discloses a Bu lactobacillus preparation that contains 1*109-1*1011 Bu lactobacillus colony forming unit. It also discloses the manufacture method and the usage. It could used to improve the quality of corn silage.

Description

technical field [0001] The invention belongs to the field of feed, and relates to a preparation of functional bacteria Lactobacillus buchneri (L.buchneri), a preparation method thereof, and an application of using the Lactobacillus buchneri preparation to improve the stability of whole plant corn silage after opening a cellar. Background technique [0002] There are many kinds of silage, which has the characteristics of wide source, low cost, convenient collection, good palatability and comprehensive nutrition. It is the main feed for herbivorous animals, such as ideal feed for cattle and sheep. General gramineous crops, leguminous crops, roots, tubers, aquatic feed and leaves can be used for silage. At present, the corn stalks, sorghum stalks and sweet potato vines that are specially planted for silage are the most used ones. [0003] The principle of silage is to produce an acidic environment through anaerobic fermentation under suitable conditions and inhibit the reprodu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/02C12R1/225A23K10/18
Inventor 管武太杨金波
Owner SOUTH CHINA AGRI UNIV
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