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130 results about "Cfu - colony-forming unit" patented technology
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In microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. Viable is defined as the ability to multiply via binary fission under the controlled conditions.
Probiotic food, process for its preparation and dietary regimen
InactiveUS20070160589A1Easy maintenanceStrong feelingBiocideMetabolism disorderWater activityDietary regimen
A probiotic food item containing a beneficial amount of dry active probiotic cultures is provided. The food item also contains a substantial continuous fat-based coating with an effectively low water activity level. The food item is packaged in a substantially moisture impermeable package marked with a use by or sell by date, so as to ensure a desired minimal amount of probiotic colony forming units (CFUs) on the use by or sell by date. Methods for manufacturing the probiotic food product are also provided.
Owner:ATTUNE FOODS
Novel compound microorganism living bacteria agent, method for preparing same and applications
The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu) / gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.
Owner:SHANGHAI ECO WELL BIOSCI
Biofunctional magnetic nanoparticles for pathogen detection
InactiveUS20060292555A1High sensitivityEasy to reportNanotechMicrobiological testing/measurementMagnetite NanoparticlesBiology
This invention provides a method of detecting pathogens comprising the steps of: (a) contacting a sufficient amount of biofunctional magnetic nanoparticles with an appropriate sample for an appropriate period of time to permit the formation of complexes between the pathogens in the sample and the nanoparticles; (b) using a magnetic field to aggregate said complexes; and (c) detecting said complexes. The method may further comprise the additional step of removing said complexes. The biofunctional magnetic nanoparticles are preferably a conjugate of vancomycin and FePt. The pathogens may be bacteria or viruses, and the sample may be a solid, liquid, or gas. Detection may involve conventional fluorescence assay, enzyme-linked immunosorbent assay (ELISA), optical microscope, electron microscope, or a combination thereof. The sensitivity of detection for the method is at least as low as 10 colony forming units (cfu) of the pathogens in one milliliter of solution within one hour.
Owner:THE HONG KONG UNIV OF SCI & TECH +1
Microbial deodorant and preparation method thereof
InactiveCN102258796AStrong fermentation and decomposition abilityGrowth inhibitionFungiBacteriaCfu - colony-forming unitSaccharomyces cerevisiae
The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit) / ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.
Owner:黄小娃
Method of determining soybean sudden death syndrome resistance in a soybean plant
InactiveUS7288386B2Solves the problem quickly and cheaply selecting resistant cultivarsImprove selection for SDS and SCN resistanceMicrobiological testing/measurementFermentationCell culture mediaBacterial growth
Owner:SOUTHERN ILLINOIS UNIVERSITY
Antibiotic dressing for the treatment of infected wounds
InactiveUS20090297588A1Simple methodExtended retention timePeptide/protein ingredientsMetabolism disorderWound dressingCfu - colony-forming unit
A silk protein membrane is described which is loaded with an antimicrobial compound and has a substantially non-granular ultrastructure which is (i) substantially devoid of micellar silk fibroin substructures and (ii) substantially devoid of pores when analysed by scanning electron microscopy at 0.2 μm resolution. The antimicrobial compound comprises, in one aspect of the invention, a host defense peptide. The silk protein membrane of the invention can be used in a method for the treatment of wounds and allows the wound dressing to be kept in place after removal of that wound dressing from a wound the wound has less than 105 colony forming units per gram. A method for manufacturing a wound dressing is also disclosed which comprises transferring a cast precursor material and optionally a host defense peptide, into a solid support and then drying the precursor material on the solid support to form a silk protein membrane for use as the wound dressing.
Owner:SPINTECH ENG
Method Of Producing Concentrated Liquid Dairy Products
InactiveUS20080160134A1Add flavorGreat tasteMilk preparationMilk preservationUltrafiltrationMicrofiltration membrane
A method is provided for forming aseptic or substantially aseptic concentrated dairy liquid, such as dairy milk, without significant heat treatment. In one form, the method first concentrates a starting dairy milk to about 2× to about 7× concentration using an ultrafiltration membrane to form a dairy concentrate. Thereafter, the dairy concentrate is filtered using a microfiltration membrane to provide the aseptic or substantially aseptic concentrated dairy milk. The resultant concentrated dairy milk has less than about 0.5 percent total bacteria and less than about 5 colony forming units of spore forming bacteria per gram. The substantially aseptic concentrated dairy milk is not subjected to significant heat treatment during processing.
Owner:INTERCONTINENTAL GREAT BRANDS LLC
Biofertilizer for improving saline and alkaline lands and preparation method and application of biofertilizer
ActiveCN105152811AImprove nutritional statusImprove fertilitySoil-working methodsFertilizer mixturesPhosphoric acidMushroom
The invention relates to biofertilizer for improving saline and alkaline lands. The biofertilizer comprises the following components in parts by weight: 200 to 250 parts of fermented mushroom residue, 150 to 200 parts of ammonium sulfate, 50 to 100 parts of superphosphate, 50 to 100 parts of complex improver, 15 to 21 parts of EDTA (ethylene diamine tetraacetic acid)-metallo-chelate, 0.5 to 1 parts of ammonium molybdate and 5 to 10 parts of borax, and also comprises 20 to 30 parts of compound micro-organism, so that the living bacteria count of the biofertilizer is not less than 5.0 * 108CFU (colony-forming unit) per gram. The invention also relates to a preparation method for the biofertilizer and the application of the biofertilizer in crop planting. The biofertilizer for improving the saline and alkaline lands, disclosed by the invention, has important functions of improving the soil conditions of the saline and alkaline lands, improving the soil fertility, increasing crop yield and preventing the soil from secondary salinization.
Owner:烟台瑞腾生物技术研究院有限公司
Method for recovering cadmium polluted soil by combining compound microorganism bacterium agent with houttuynia cordata
InactiveCN103071672AImprove the effect of bioremediationImprove repair effectContaminated soil reclamationHouttuyniaCombined method
The invention discloses a method for recovering cadmium polluted soil by combining a compound microorganism bacterium agent with houttuynia cordata. The compound microorganism bacterium agent comprises the following components by count: 1*10<9>-9*10<9>CFU (colony forming unit) / m<3> pseudomonas fluorescens, 1*10<8>-6*10<8>CFU / m<3> actinomyces ruminicola, and 1*10<9>-9*10<9>CFU / m<3> glomus mosseae. The method for recovering the soil comprises the following steps that the houttuynia cordata is planted in cadmium soil and the compound microorganism bacterium agent is inoculated into the soil, or the houttuynia cordata is soaked in the compound microorganism bacterium agent and then planted into the cadmium soil; when branches and leaves of the houttuynia cordata are luxuriant, the branches and the leaves of the houttuynia cordata are harvested; and cadmium in the soil can be removed. The compound microorganism bacterium agent can better improve the tolerance of the houttuynia cordata, promote growth of a plant in the high-cadmium-content soil, improve the absorbility of the plant to cadmium, and enhance a soil heavy metal removal effect. The method for recovering the soil is a safe, environment-friendly, high-efficiency, economical and practical plant-microorganism combined method for recovering the heavy metal polluted soil, and has a significant application value.
Owner:SICHUAN UNIV
Treatment of inflammation, respiratory tract infections and cystic fibrosis
InactiveUS20170239289A1Lower Level RequirementsAntibacterial agentsInorganic active ingredientsDiseaseVolumetric Mass Density
The present invention provides a method for treating a disease in a human subject in need thereof, wherein the disease is selected from the group consisting of inflammation, bronchiolitis and cystic fibrosis, and wherein the method comprises repeatedly administering to the human subject a gas mixture comprising nitric oxide at a concentration from about 144 to about 176 ppm for a first period of time, followed by a gas mixture containing no nitric oxide for a second period of time, wherein the administration is repeated for a time sufficient to: a) reduce the level of at least one inflammatory biomarker in the human subject when compared to the level of the inflammatory biomarker prior to the administration; b) reduce the microbial density by 1 to 2 log units as measured by colony forming units in the human subject when compared to the microbial density prior to the administration; or c) a combination thereof.
Owner:ADVANCED INHILATION THERAPIES AIT LTD
Production method of microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes
The invention discloses a production method of a microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The production method is characterized by comprising the following steps: carrying out symbiotic assemblage screening on lactic acid bacteria and saccharomycetes separated from an Inner Mongolia dairy product kumiss to select a set of enterococcus faecium HE5 and brewer yeast J-4 with mutual promotion, wherein the viable count of the lactic acid bacteria reaches 2.88*10<9>CFU (Colony-Forming Unit) / mL when enterococcus faecium HE5 and brewer yeast J-4 screened are cultivated on an optimized skimmed milk culture medium, the viable count of the saccharomycetes reaches 3.3*10<8>CFU / mL, and double bacteria culture is better to promote the growing effect of lactic acid bacteria; and freezing and drying the cultivated and harvested double bacteria to obtain the microbial preparation by symbiotic fermentation of lactic acid bacteria and saccharomycetes. The microbial preparation is good in symbiotic effect and strong in acid-producing capacity.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
Straw in-situ decomposition microbial agent and application thereof
InactiveCN103642721AIncrease enzyme activityQuick destructionBio-organic fraction processingBacteriaCelluloseMicrobial agent
The invention discloses a straw in-situ decomposition microbial agent. Active ingredients of the microbial agent are formed by mixing clostridium xylanolyticum, xylan monad cellulolyticus, pseudomonas and bacillus subtilis in a CFU (Colony Forming Unit) ratio of (25-35): (25-30):(20-30):(15-20). Experiments prove that the microbial agent provided by the invention has degradation rate for straws, which can reach 70%-80% in a culture medium, and can reach over 50% when the straws are returned to the field for 20 days. The microbial agent disclosed by the invention is low in cost and simple to prepare. The microbial agent disclosed by the invention breaks through the limit that natural cellulose cannot be efficiently decomposed by purifying bacteria and clastic enzymes thereof, provides a key technology for straw in-situ rapid decomposition, and has a wide application prospect in the field of returning straws to the field and decomposing a lignocelluloses material.
Owner:MOUNT EMEI GREEN LAND ECOLOGICAL AGRI DEV LIMITED
Silage inoculant and application thereof
InactiveCN104531566ALower pHImprove fermentation qualityBacteriaMicroorganism based processesFungicideLactococcus
The invention relates to a silage inoculant and a preparation method and application of the silage inoculant. The silage inoculant is prepared by respectively fermenting lactobacillus plantarum, bacillus subtilis and lactococcus and mixing the obtained fermentation broth, wherein the ratio of numbers of colony-forming units of lactobacillus plantarum to bacillus subtilis to lactococcus lactis in the mixed fermentation broths is (90-96) to (3.5-6) to (0.5-4), and the total number of colony-forming units is 0.8*10<12>-1.2*10<12>CFU / ml. Various lactic acid bacteria in the fungicide have a high synergistic effect, and form a stable eco-system, and non-sterile natural raw materials can be quickly propagated and colonized to hold advantages, so that large amounts of lactic acid are quickly generated, the pH value of fermented materials is reduced, the fermentation quality of a fodder is improved, the nutritional value and the palatability of the fodder are improved, the application is simple and easy, and the cost is low.
Owner:CHINA AGRI UNIV
Biofunctional magnetic nanoparticles for pathogen detection
InactiveUS7754444B2High affinityBig ratioNanotechMicrobiological testing/measurementMagnetite NanoparticlesBiology
Owner:THE HONG KONG UNIV OF SCI & TECH +1
Bushi lactobacillus prepn., prepn. method and use thereof
InactiveCN1831111AImprove stabilityDefuse security risksBacteriaAnimal feeding stuffBiotechnologyLactobacillus
The invention discloses a Bu lactobacillus preparation that contains 1*109-1*1011 Bu lactobacillus colony forming unit. It also discloses the manufacture method and the usage. It could used to improve the quality of corn silage.
Owner:SOUTH CHINA AGRI UNIV
Method for culturing hematopoietic stem/progenitor cells
InactiveCN101597594AA large amountGood ability to rebuild bloodTissue cultureProgenitorBiotechnology
The invention belongs to the field of biotechnology, and relates to a method for expanding the hematopoietic stem / progenitor cells from umbilical cord blood in vitro by an alginate three-dimensional culture system. The method combines SCF, TPO and FL cytokines to support the expansion of the hematopoietic stem / progenitor cells in a single karyocyte of human umbilical cord blood, does not need adding other cytokines, and cultures the single karyocyte of human umbilical cord blood packaged in an alginate microsphere in a three-dimensional stationary culture system and a three-dimensional rotary culture system to observe the expansion effect of the single karyocyte of human umbilical cord blood packaged in the alginate microsphere in the rotary culture system. Compared with the conventional two-dimensional system, the three-dimensional culture system improves the total cells and obviously increases CD34+ cells under the condition of culturing low-concentration cytokines; and colony forming unit-granulocytes and macrophages of cells expanded in the three-dimensional culture system are cloned to form obviously increased cells, and the single karyocyte of human umbilical cord blood in a transplanted NOD / SCID mouse has better engraftment and reconstructs hemopoiesis.
Owner:AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis
ActiveCN102559560AHigh content of live bacteriaGuaranteed validityBacteriaWaste water treatment from animal husbandryBacillus licheniformisMicroorganism
The invention relates to the field of microorganism application, in particular to Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparations and application of the Bacillus licheniformis. The invention provides Bacillus licheniformis CRVAB001 for producing lactic acid at high yield after fermentation in the collection number of CGMCC No.4850. The inventionalso provides a microecological liquid preparation comprising the Bacillus licheniformis, a preparation method for the microecological liquid preparation, microecological powder comprising the Bacillus licheniformis, and a preparation method for the microecological powder. The Bacillus licheniformis can be efficiently and quickly fermented to produce the lactic acid, and has great industrial application prospect. The viable content of the liquid preparation is more than 1*10<10> colony-forming units (CFU) / mL, the viable content of the powder is more than 1*10<11>CFU / g, and the effectiveness of the Bacillus licheniformis preparations is fully guaranteed.
Owner:北京科为博生物科技有限公司
High pressure processing of foods
High pressure processing (HPP) uses isostatic pressure to evenly apply great pressures to foods on all sides of the foods. Because the pressure is isostatic, the food is not deformed, so long as there is no internal cavity in the food. The pressures used are preferably from about 100 MPa (about 14.5 ksi) to about 1000 MPa (about 145 ksi), and the temperature rise is preferably limited to about 3° C. (about 5° F.) per 100 MPa (about 14.5 ksi). The high pressure dramatically reduces pathogens in the food so processed, as much as a four or five-log reduction in counts of colony forming units (CFU). The process has been shown to be effective in achieving these reductions in E. Coli, listeria, and salmonella. Pieces or sticks of beef jerky or sausage may be processed via HPP and combined with cheese to form a food product with a long shelf life.
Owner:SNACK PATROL
Probiotic composition and preparation method and application thereof
ActiveCN113151056ASurprised by the technical effectRelieve constipation somatosensory effectBacteriaDigestive systemBiotechnologyProbiotic bacterium
The invention provides a probiotic composition and a preparation method and application thereof. The probiotic composition comprises raw materials of lactobacillus reuteri, lactobacillus acidophilus and bifidobacterium animalis, wherein the weight ratio of the lactobacillus reuteri to the lactobacillus acidophilus to the bifidobacterium animalis is (1.5-2.5):(0.5-1.5):(0.5-1.5). The colony forming units of the lactobacillus reuteri, the lactobacillus acidophilus or the bifidobacterium animalis in unit weight are the same. The lactobacillus reuteri is preserved in China Center for Type Culture Collection with the preservation number of 209138, and the lactobacillus acidophilus and / or the bifidobacterium animalis preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC 15210 are / is bifidobacterium animalis BB-115. The probiotic composition can relieve the symptom of slow transit constipation under the condition of low dosage, and can remarkably and effectively regulate gastrointestinal regulatory peptide indexes related to constipation, such as P substance (SP), gastrin (Gas) and vasoactive peptide (VIP).
Owner:SIRIO PHARMA CO LTD
Polycyclic aromatic hydrocarbon antibacterial agent of composite carrier and preparation method of polycyclic aromatic hydrocarbon antibacterial agent
InactiveCN102392012ALittle heterogeneityHigh activityContaminated soil reclamationOn/in inorganic carrierPolycyclic aromatic hydrocarbonPolluted environment
The invention discloses a polycyclic aromatic hydrocarbon antibacterial agent of a composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent comprises a Mycobacterium vanbaalenii. PYR-1 bacterium suspension and a carrier, wherein the volume / mass ratio of the bacterium suspension to the carrier is (20-50)ml: 15g; (5.0-6.0)*10<8>CFU (Colony-Forming Unit) of the Mycobacterium vanbaalenii. PYR-1 thallus is contained in the bacterium suspension per ml; the carrier comprises black charcoal and humus in the mass ratio of (1.8-2.2):1. The invention also discloses a preparation method of the polycyclic aromatic hydrocarbon antibacterial agent of the composite carrier. The polycyclic aromatic hydrocarbon antibacterial agent is capable of effectively reducing the content of the polycyclic aromatic hydrocarbon in the polluted environment.
Owner:ZHEJIANG UNIV
Bacterial cultivation system for growth of substrate specific micro-organisms for use in industrial wastewater remediation
ActiveUS8052873B1Adequate air introductionWater treatment compoundsMultistage water/sewage treatmentCell massWaste treatment
A waste treatment method includes the concentration of selected strains of bacteria in a selected medium in the presence of nutrients and water, under aerobic conditions. This concentrated batch is thereafter discharged for downstream applications in wastewater remediation. The present invention employs a cultivation chamber having inlet ports and a circular vent port that allows for adequate air introduction and heat release. Aeration is achieved by recirculation of the fluid medium from the top of the apparatus through a pipe that runs the length of the inner wall and is specially configured at the top to minimize cell damage. Fluid can be routed tangentially in both the clockwise and counterclockwise directions within the chamber. The conical bottom also has an orifice allowing for recirculation of the fluid medium tangentially to the sidewalls. Upon completion of the batch cultivation, the medium and bacteria are discharged for downstream applications in wastewater remediation of paper mill, chemical plant, oil refinery, and other industrial effluents. The aeration and circulation of the fluids is designed to limit cells shearing and damage yet achieve critical cell mass (e.g. between 107 and 1010 cfu (colony forming units) per milliliter (ml)) and dissolved oxygen uptake rates (e.g. between about 50 and 1500) during cultivation.
Owner:ENVIRONMENTAL BUSINESS SPECIALISTS
Formula and preparation method of yeast liquid biocontrol bactericide
InactiveCN103583576AReduce incidenceImprove the effect of prevention and controlBiocideFungicidesSucrosePhosphate
The invention discloses a formula and a preparation method of a yeast liquid biocontrol bactericide. The formula comprises yeasts, sucrose, trehalose, galactose, ascorbic acid and a 0.05 mol / L phosphate buffer solution. The method comprises the following steps: activating a yeast strain 4-4A by an NYDB (nutrient yeast dextrose broth) liquid culture medium and inoculating two rings into 50 ml of a seed culture medium; putting a cultured yeast fermentation solution into 50 ml of a centrifugal pipe for conducting a centrifuging process at a speed of 4000 r / min for 15 minutes, dumping supernatant liquor, washing by aseptic water for two times, and adding obtained sediments into the 0.05 mol / L phosphate buffer solution to prepare a bacterial suspension solution; selecting the sucrose, the galactose and the trehalose as protecting agents for preparing a solution by the phosphate buffer solution; adding 21.6 ml of the solution into 2.4 ml of a yeast mother solution with 109 CFU / ml (colony forming unit / ml) of the yeasts, fully and uniformly mixing, and sub-packaging a mixture into 1.5 ml of the centrifugal pipe; measuring the number of living yeasts in a yeast preparation. According to the yeast liquid biocontrol bactericide, the occurrence rate of anthracnose after mangoes are harvested is effectively reduced, the yeast liquid biocontrol bactericide is applied for 4-6 times from an early flowering period to a harvest period to prevent and control mango anthracnose, the morbidity and the severity are reduced by 64%-67% and 89%-90% respectively, and the disease prevention and control effect is improved.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Improved meat slurry methods of production and compositions
InactiveUS20160029667A1Sufficient amountSmall sizeMilk preparationAnimal feeding stuffBiotechnologyAnimal protein
A process for producing a meat slurry is described. The process includes: (i) obtaining an animal source of protein; (ii) reducing piece size of the animal source of protein to produce a smaller-piece-sized animal source of protein; (iii) introducing one or more types of lactic acid producing bacteria and at least one carbohydrate energy source to the smaller-piece-sized animal source of protein to produce a meat mixture; (iv) adding water to the meat mixture to produce an activated meat mixture; (v) incubating the activated meat mixture to produce a meat slurry; and wherein the activated meat mixture has a pH value that is greater than about 5.0, and wherein prior to incubating, the lactic acid producing bacteria is present on the meat mixture in an amount that is at least about 1×107 colony forming units (“CFU”) of lactic acid producing bacteria per gram of said meat mixture, and wherein after incubating, the lactic acid producing bacteria produces lactic acid in an amount that is sufficient to maintain a pH of the meat slurry at a value that is no greater than about 4.7.
Owner:MICRO NATURE
Stabilizing methods for coating seeds with biological materials
The present invention provides a method for coating seeds with biological materials such as bacteria, fungi (e.g., yeasts and molds), parasites, recombinant vectors, and viruses. The method comprises (a) applying a moistening liquid to seeds to moisten seeds, wherein the moistening liquid comprises a moistening polymer, and (b) coating the moistened seeds with an effective amount of a dry composition, wherein the dry composition comprises biological materials, one or more disaccharides, one or more oligosaccharides, one or more polysaccharides, one or more carboxylic acid salts, and one or more hydrolyzed proteins. The coated seeds may have an initial water activity (Aw) below 0.4, and the microorganisms on the coated seeds have initial viability of at least 5 logs of colony forming units per gram of seeds (CFU / g seed). Also provided are coated seeds.
Owner:ADVANCED BIONUTRITION CORP
Pleurotus eryngii yoghurt and production method thereof
InactiveCN103444869AIncrease health functionPromoting rational processing and utilizationMilk preparationAdditive ingredientProduct processing
The invention provides pleurotus eryngii yoghurt and a production method thereof, and belongs to the technical field of food science. The pleurotus eryngii yoghurt is milk white in color, has uniform and exquisite texture, moderate viscosity, faint fragrance of pleurotus eryngii and special aroma of fermented milk, and moderate soursweet, and contains not less than 3.0% of protein, not less than 3.1% of fat, not greater than 70 thorner degrees of acidity, and not less than 1*10<6>CFU (Colony Forming Unit) / mL of lactobacilli count. The production method comprises the following steps: getting pleurotus eryngii and milk as the major raw materials; slicing and pre-cooking the pleurotus eryngii; pulping the pleurotus eryngii with equivalent water; adding milk, granulated sugar, a stabilizer and a sweetening agent in proportion; homogenizing; sterilizing; cooling; adding 2.5% of lactobacillus bulgaricus and streptococcus thermophilus in proportion of 1: 1.5; filling; fermenting at 42 DEG C until the titration acidity reaches 65 to 75 thorner degrees; and stopping to refrigerate and after-ripen for 24 hours at 4 to 6 DEG C to obtain the pleurotus eryngii yoghurt. The production method has the beneficial effects that the whole pleurotus eryngii is pulped and added, thus the variety and content of nutritional components in the yoghurt are increased, the healthcare function of the yoghurt is improved, and new taste is created. The production method is important for promoting the rational processing and utilizing of pleurotus eryngii resources, formation of an after-production processing industry chain and adjustment of an agriculture product processing industry structure.
Owner:HEBEI AGRICULTURAL UNIV.
Additive having function of regulating intestines and stomach of pet as well as preparation method and application thereof
InactiveCN108056226ASimple structureHigh glossFood processingDigestive systemIntestinal structureAnti stress
The invention provides an additive having a function of regulating intestines and stomach of a pet as well as preparation method and application thereof, and belongs to the technical field of animal feed. The additive having the function of regulating the intestines and the stomach of the pet is prepared from the following components in parts by weight: 2 to 10 parts of lactobacillus acidophilus powder, 1 to 5 parts of fructus crataegi, 2 to 10 parts of rhizoma dioscoreae, 2 to 10 parts of poria cocos, 1 to 5 parts of liquorice root and 1 to 5 parts of pericarpium citri reticulatae, wherein the viable bacteria concentration of the lactobacillus acidophilus powder is 0.5*10<10> to 1.5*10<10> CFU (Colony Forming Unit) / g. The additive provided by the invention is capable of obviously increasing anti-stress capability of puppies in a weaning stage and regulating the gastrointestinal function, an experiment shows that compared with a control group, the prevalence rate, the appetite, the skin and hair glossiness, the activity, the daily gain and the intestinal flora composition of an experimental group are all remarkably improved, and compared with a rhizoma dioscoreae and poria cocos taste-lack group, the prevalence rate, the appetite, the skin and hair glossiness, the activity and the daily gain of the experimental group have remarkable difference.
Owner:JILIN UNIV
Straw decomposition agent
InactiveCN106495761ARipe fastEfficient decompositionBio-organic fraction processingExcrement fertilisersDiseaseDecomposition
The invention discloses a straw decomposition agent. An active ingredient of the straw decomposition agent is prepared from actinomycetes, trichoderma harzianum, saccharomyces and bacillus subtilis which are in the bacterial flora formation unit number ratio of (0.5 to 2): (1 to 2): (0.8 to 1.2): 1. According to the straw decomposition agent disclosed by the invention, all bacterial florae can harmoniously coexist and mutually promote so as to achieve the effect of having complementary advantages, and lignin, cellulose, hemicellulose and the like in crop straws can be effectively decomposed; the degradation time is short, the decomposition effect is stable, diseases and insect pests are eliminated effectively, and the straw decomposition agent has a decomposition effect at normal temperatures, so that the rapid treatment of organic materials and final resource-converting utilization are achieved; treated organic solid wastes are transformed thoroughly, embody good properties of improving soil, promoting growth and inhibiting soil-borne diseases and meanwhile are environmentally friendly and sanitary.
Owner:临沂中磷生物科技有限公司
Thorough decomposition agent for locally returning straw to fields, and preparation method of thorough decomposition agent
PendingCN110628675AImprove decomposition efficiencyShort degradation timeFungiBio-organic fraction processingDecompositionPotassium
The invention relates to a thorough decomposition agent for locally returning straw to fields, and a preparation method of the thorough decomposition agent, and belongs to the technical field of thorough decomposition agents for straw. The thorough decomposition agent disclosed by the invention has the characteristics of being high in decomposition and disintegration efficiency and short in degradation time, and particularly has favorable disintegration effects at normal temperature and even low temperature. The thorough decomposition agent for locally returning straw to fields, disclosed by the invention, is prepared through the steps of performing fermentation with bacillus amyloliquefaciens, aspergillus niger, trichoderma longibrachiatum and saccharomyces cerevisiae respectively to obtain fermentation liquid, and mixing the fermentation liquid, wherein the ratio of the unit number of colonies of bacillus amyloliquefaciens agent fermentation liquid to the unit number of colonies of aspergillus niger agent fermentation liquid to the unit number of colonies of trichoderma longibrachiatum agent fermentation liquid to the unit number of colonies of saccharomyces cerevisiae fermentation liquid is 2:3:3:1. The thorough decomposition agent for locally returning straw to fields, obtained by the preparation method disclosed by the invention can promote rapid thorough decomposition ofthe straw and enable the thoroughly decomposed straw to return to the fields, can supplement soil organic matter, can sufficiently release and utilize nitrogen, phosphorus and potassium nutrients in the straw, and besides, can improve soil microorganism environment.
Owner:哈尔滨谷润生态科技发展有限公司
Methods for co-culturing cord blood derived cells with menstrual stem cells
InactiveUS20090191628A1Increased proliferationArtificial cell constructsSkeletal/connective tissue cellsPopulation doublingCord blood stem cell
Methods are provided for obtaining expanded human cord blood cells expressing CD34. The methods involve seeding a sufficient amount of cord blood cells with a sufficient amount of menstrual cells under co-culture conditions suitable to promote expansion of the cord blood cells, and co-culturing the cord blood cells with the menstrual cells under culture conditions that support at least two or more population doublings of the cord blood cells. Methods are also provided for growing expanded human cord blood cells to give rise to any one of colony forming units, colony forming unit granulocyte macrophages (CFU-GM), burst forming unit erythroids (BFU-E), and colony forming unit granulocyte erythrocyte macrophage megakaryocyte (CFU-GEMM) blood lineage precursor cells. The expanded cells may express CD34, SSEA-4, and HLA-II. Compositions of the expanded cells are also provided.
Owner:CRYO CELL INTERNATIONAL INC
Microbial powder, infant yoghourt and preparation method thereof
ActiveCN103468617AHigh in fatProduct properties are stableMilk preparationBacteriaMicroorganismColony-forming unit
The invention discloses a microbial powder, an infant yoghourt and a preparation method thereof. The microbial powder comprises a compound microbial powder of streptococcus thermophilus STI-13 and streptococcus thermophilus Body-3 at a ratio of 1:1 to 1:4 by colony forming unit quantity. The infant yoghourt obtained by fermentation by using the microbial powder is free of D-lactic acid, and the protein component, the fat structure component and the fatty acid component are all similar to those of breast milk, therefore the infant yoghourt can be favorably digested and assimilated by infants.
Owner:BRIGHT DAIRY & FOOD
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