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Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis

A Bacillus licheniformis and post-fermentation technology, applied in the directions of application, bacteria, chemical instruments and methods, etc., can solve the problems of unfavorable large-scale production and popularization, fast acid production rate, and high number of viable bacteria, and achieve strong industrial application prospects and easy processing. and preservation, the effect of improving the content of viable bacteria

Active Publication Date: 2012-07-11
北京科为博生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Liquid fermentation is easy to control and reduces pollution, but the patent does not disclose the number of live bacteria of Bacillus licheniformis fermented by this technology
Patent CN1202522A discloses a strain of Bacillus licheniformis and its probiotics. This method uses solid fermentation method or liquid fermentation method to ferment Bacillus licheniformis. The operation is simple and easy to implement, but this method belongs to the stage of laboratory research and development, which is not conducive to large-scale production. to promote
Patent CN102021131A discloses a preparation method of Bacillus licheniformis liquid preparation, liquid submerged fermentation, the number of viable bacteria is 2×10 9 When CFU / mL, the required fermentation liquid is obtained. This method is a typical liquid fermentation, and the fermentation liquid can be used to prevent and control watermelon wilt; the liquid fermentation method is not easy to infect bacteria, but the number of viable bacteria prepared by this process is low
Patent CN102120972A discloses a preparation method of Bacillus licheniformis, which uses three temperature gradients to resuscitate the freeze-dried bacteria powder of Bacillus licheniformis. The fermented liquid is dried by hot air circulation at 60±2°C, and the Bacillus licheniformis powder prepared after three-stage resuscitation has a high number of viable bacteria, and the post-treatment operation of this fermentation method is simple, but the heat drying takes too long and the viable bacteria large loss
Wang Xinlei et al. (2010) studied the fermentation conditions for Bacillus licheniformis to transform starch to produce lactic acid. After optimization, the amount of lactic acid produced was high and the rate of acid production was fast, but the number of viable bacteria and the rate of spore formation were not disclosed.

Method used

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  • Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis
  • Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis
  • Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparation and application of Bacillus licheniformis

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Effect test

Embodiment 1

[0050] Example 1 Bacillus licheniformis CRVAB001 (Bacillus licheniformis) strain isolation, purification

[0051] Samples were collected from the gastrointestinal tract of broiler chickens, layer hens, piglets, and sows, and microorganisms were isolated and purified in the laboratory. 114 strains of Bacillus were isolated from them. Twelve strains of Bacillus were screened out by the reverse test, and one high-yielding lactic acid strain was screened out by the lactic acid production test, and its lactic acid content could reach 20 μg / mL as determined by gas chromatography. The strain number is CRVAB001, identified as Bacillus licheniformis by 16S rRNA gene sequence. The strain was preserved on May 17, 2011 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, 100101), and its preservation number is: CGMCC No. 4850....

Embodiment 2

[0052] The biological characteristics of embodiment 2 Bacillus licheniformis CRVAB001 (Bacillus licheniformis)

[0053] Bacterial morphology and physical and chemical characteristics were identified according to the "Bergey's Bacterial Identification Manual". The test results of the micromorphology and physicochemical characteristics of the Bacillus licheniformis CRVAB001 are shown in Table 1 below.

[0054] Table 1 Bacillus licheniformis bacterial morphology and physicochemical test results

[0055] Pilot projects

Embodiment 3

[0056] Embodiment 3 prepares novel bacillus licheniformis liquid agent-Y1

[0057] 1) Primary slant culture of Bacillus licheniformis: inoculate Bacillus licheniformis CRVAB001 on the slant medium by conventional methods, and cultivate at 30° C. for 36 hours to obtain the primary slant surface of Bacillus licheniformis; the medium is prepared according to the following ratio Mixture (g / L): beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, agar 20g / L, water 1000mL, pH 7.0-7.5;

[0058] 2) Secondary liquid culture of Bacillus licheniformis: Inoculate the primary slant surface of Bacillus licheniformis obtained in step 1) into the liquid medium, and cultivate it under the conditions of 30°C and 250r / min to obtain the Bacillus licheniformis liquid, with the number of viable bacteria ≥ 1 ×10 10 CFU / mL stop culture; Wherein, by weight percentage, described fermentation culture medium is the mixed solution (%) prepared according to following ratio: corn meal 4.0%, soybean meal...

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Abstract

The invention relates to the field of microorganism application, in particular to Bacillus licheniformis for producing lactic acid at high yield after fermentation, and preparations and application of the Bacillus licheniformis. The invention provides Bacillus licheniformis CRVAB001 for producing lactic acid at high yield after fermentation in the collection number of CGMCC No.4850. The inventionalso provides a microecological liquid preparation comprising the Bacillus licheniformis, a preparation method for the microecological liquid preparation, microecological powder comprising the Bacillus licheniformis, and a preparation method for the microecological powder. The Bacillus licheniformis can be efficiently and quickly fermented to produce the lactic acid, and has great industrial application prospect. The viable content of the liquid preparation is more than 1*10<10> colony-forming units (CFU) / mL, the viable content of the powder is more than 1*10<11>CFU / g, and the effectiveness of the Bacillus licheniformis preparations is fully guaranteed.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a fermented bacillus licheniformis with high lactic acid production and its preparation and application. Background technique [0002] Bacillus licheniformis (Bacillus licheniformis) is one of the feed-grade strains approved by the Ministry of Agriculture in 2008 No. 1126. Bacillus licheniformis is a facultative anaerobic microorganism capable of producing spores. The bacteria reproduce fast, have strong stress resistance, and can secrete protease, amylase, lipase and various amino acids. Compared with lactic acid bacteria and bifidobacteria, the advantage of Bacillus is that it has strong resistance to external adverse environments. Compared with Bacillus subtilis, which is also a Bacillus, the growth temperature of Bacillus licheniformis is 5-7°C higher; Secondly, its growth rate is moderate, and it is easy to fully fold unfolded proteins that have just crossed the me...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C02F3/34C12R1/10C02F103/20
Inventor 吴培均姚琨李富伟杨忠广李兆勇
Owner 北京科为博生物科技有限公司
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