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Silage inoculant and application thereof

An inoculant and silage additive technology, which is applied in the application, microorganism, preservation method of animal feed raw materials, etc., can solve the problems of high cost and poor aerobic stability of silage, and can reduce pH value, improve nutritional value and palatability. , the effect of animal safety

Inactive Publication Date: 2015-04-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of the inoculant is better than that of CN101449752A, but the homofermentative lactic acid bacteria produce a large amount of lactic acid, and the aerobic stability of the silage is poor
Add the inoculant to switchgrass silage, after 30 days of fermentation, the pH of the silage drops to 4.2, but the cost of cellulase is high

Method used

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  • Silage inoculant and application thereof

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1 A kind of preparation of silage inoculant

[0025] A silage inoculant, inoculate Lactobacillus plantarum, Bacillus subtilis, and Lactococcus lactis in MRS-S medium respectively, culture at 30°C for 72 hours, and mix the obtained fermentation liquid in proportion, wherein Lactobacillus plantarum, Bacillus subtilis , The colony-forming unit ratio of Lactococcus lactis is 94:4.5:1.5, and the total number of colony-forming units is 1×10 12 CFU / ml, the silage inoculant is obtained.

Embodiment 2

[0026] Embodiment 2 A kind of preparation of silage inoculant

[0027] A silage inoculant, inoculate Lactobacillus plantarum, Bacillus subtilis, and Lactococcus lactis in MRS-S medium respectively, culture it statically at 32°C for 70 hours, and mix the obtained fermentation liquid in proportion, wherein Lactobacillus plantarum, Bacillus subtilis , The colony-forming unit ratio of Lactococcus lactis is 92:6:2, and the total number of colony-forming units is 1×10 12 CFU / ml, the silage inoculant is obtained.

Embodiment 3

[0028] Embodiment 3 A kind of preparation of silage inoculant

[0029] A silage inoculant, inoculate Lactobacillus plantarum, Bacillus subtilis, and Lactococcus lactis in MRS-S medium respectively, culture at 31°C for 75 hours, and mix the obtained fermentation liquid in proportion, wherein Lactobacillus plantarum, Bacillus subtilis , The colony-forming unit ratio of Lactococcus lactis is 90:6:4, and the total number of colony-forming units is 1×10 12 CFU / ml, the silage inoculant is obtained.

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Abstract

The invention relates to a silage inoculant and a preparation method and application of the silage inoculant. The silage inoculant is prepared by respectively fermenting lactobacillus plantarum, bacillus subtilis and lactococcus and mixing the obtained fermentation broth, wherein the ratio of numbers of colony-forming units of lactobacillus plantarum to bacillus subtilis to lactococcus lactis in the mixed fermentation broths is (90-96) to (3.5-6) to (0.5-4), and the total number of colony-forming units is 0.8*10<12>-1.2*10<12>CFU / ml. Various lactic acid bacteria in the fungicide have a high synergistic effect, and form a stable eco-system, and non-sterile natural raw materials can be quickly propagated and colonized to hold advantages, so that large amounts of lactic acid are quickly generated, the pH value of fermented materials is reduced, the fermentation quality of a fodder is improved, the nutritional value and the palatability of the fodder are improved, the application is simple and easy, and the cost is low.

Description

technical field [0001] The invention relates to a silage inoculant and an application thereof, belonging to the field of silage processing. Background technique [0002] In recent years, with the development of my country's animal husbandry, the rapid development of grassland forage industry has been driven. High-quality grass forage is more and more favored by people. However, as the number of livestock continues to increase, the natural growth of these pastures can no longer meet the nutritional needs of livestock throughout the year. Therefore, producers solve this contradiction by artificially planting high-quality and high-yield pastures. Gramineous forages such as switchgrass have high productivity, excellent forage characteristics and soil and water conservation capabilities, and strong stress resistance. They can be planted in arid and semi-arid areas, low-lying areas prone to waterlogging and saline-alkali, and mountainous and semi-mountainous areas with poor soil....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K3/02C12R1/25C12R1/125C12R1/01
CPCC12N1/205C12R2001/01C12R2001/125C12R2001/25
Inventor 王小芬崔宗均刘晶晶袁旭峰朱万斌
Owner CHINA AGRI UNIV
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