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Microbial inoculum for degrading straw

A technology of bacteria agent and straw, applied in the direction of microorganism-based methods, bacteria, fungi, etc., can solve the problems of low energy value, unbalanced mineral content, low protein content, etc., and achieve the effect of improving quality

Inactive Publication Date: 2010-02-03
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the shortcoming of chemical method is: cellulose content is too high, and protein content is low (about 5%-10%); Mineral content is unbalanced, lacks vitamin A, vitamin D and vitamin E etc.; , 2004; Yang Lianyu, 2001)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the preparation of the bacterial agent for degrading straw

[0017] The bacteria agent used to degrade straw consists of reagent A, reagent B and reagent H, and reagent A, reagent B and reagent H are packaged independently. Reagent A, Reagent B and Reagent H were prepared as follows:

[0018] Strain fermentation medium: cornmeal 0.5g / L, corn stalk powder 15g / L, bran 0.25g / L, bean cake powder 0.25g / L, 4ml / 100ml Vogel’s mother liquor, pH 8.5.

[0019] Trichoderma viride (Trichoderma viride) ACCC 30166 was cultivated in the fermentation medium of the strain at 28° C., shaken at 200 r / min for 3 days, and all the fermentation liquid was collected as reagent A.

[0020] Trichoderma aureoviride (Trichoderma aureoviride) ACCC 32248 was cultivated in the fermentation medium of the strain at 28° C., shaken at 200 r / min for 3 days, and all the fermentation liquid was collected as reagent B.

[0021] Bacillus paenibacillus pabuli (Bacillus paenibacillus pabuli) ACCC...

Embodiment 2

[0030] Embodiment 2, the preparation of the bacterial agent for degrading straw

[0031] The bacterial agent for degrading straw is composed of reagent A, reagent B and reagent H, and the reagent A, reagent B and reagent H are uniformly mixed to prepare the bacterial agent for degrading straw.

[0032] Reagent A, Reagent B and Reagent H were prepared as follows:

[0033] Strain fermentation medium: cornmeal 0.5g / L, corn stalk powder 15g / L, bran 0.25g / L, bean cake powder 0.25g / L, 4ml / 100ml Vogel’s mother liquor, pH 8.5.

[0034] Trichoderma viride (Trichoderma viride) ACCC 30166 was cultivated in the fermentation medium of the strain at 28° C. and shaken at 200 r / min for 3 days, and all the fermentation liquid was collected as reagent A.

[0035] Trichoderma aureoviride (Trichoderma aureoviride) ACCC 32248 was cultivated in the fermentation medium of the strain at 28° C. and shaken at 200 r / min for 3 days, and all the fermentation liquid was collected as reagent B.

[0036] Fee...

Embodiment 3

[0038] Embodiment 3, the bacterial agent for degrading straw

[0039] The bacteria agent for degrading straw consists of reagent A, reagent B and reagent H, and the reagent A, reagent B and reagent H are packaged separately. Reagent A, Reagent B and Reagent H were prepared as follows:

[0040] Strain fermentation medium: cornmeal 0.5g / L, corn stalk powder 15g / L, bran 0.25g / L, bean cake powder 0.25g / L, 4ml / 100ml Vogel’s mother liquor, pH 8.5.

[0041] Trichoderma viride (Trichoderma viride) ACCC 30166 was cultivated in the fermentation medium of the strain at 28° C. and shaken at 200 r / min for 5 days, and all the fermentation liquid was collected as reagent A.

[0042] Trichoderma aureoviride (Trichoderma aureoviride) ACCC 32248 was cultivated in the fermentation medium of the strain at 28° C. and shaken at 200 r / min for 5 days, and all the fermentation liquid was collected as reagent B.

[0043] Feed Paenibacillus pabuli (Paenibacillus pabuli) ACCC 10273 was cultivated in th...

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PUM

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Abstract

The invention discloses a microbial inoculum for degrading straw and the active ingredients of the microbial inoculum comprise Trichoderma viride, Trichoderma aureoviride and forage Paenibacillus pabuli. The colony formation unit ratio of Trichoderma viride to Trichoderma aureoviride and forage Paenibacillus pabuli in the microbial inoculum is (0.8-1.2):(0.8-1.2):(0.8-1.2). The microbial inoculumfor degrading straw of the invention can mature straw fast to prepare organic fertilizer, and the prepared organic fertilizer can be uses as a base fertilizer and can obviously improve the quality ofvegetables.

Description

technical field [0001] The invention relates to a bacterial agent for degrading straw. Background technique [0002] Crop straw is a potential non-competitive resource, which has the characteristics of large quantity, wide distribution and variety. According to reports, the total annual output of various crop straws in my country is more than 700 million tons, including 230 million tons of rice straw, 220 million tons of corn straw, 1 billion tons of beans and autumn miscellaneous grains, and 100 million tons of peanuts, potato vines and beet leaves. tons, accounting for about 30% of the world's total straw production. However, these straw resources have not been rationally developed for a long time. Traditionally, straw is used as fuel for farmers or roughage for livestock, and a small amount is used for papermaking. In recent years, due to the sharp decline in the living fuel of farmers, the amount of direct or decomposed straw returning to the field is small, while the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C05F11/08C12R1/885C12R1/01
Inventor 顾金刚姜瑞波李世贵张晓霞阮志勇马晓彤张瑞颖
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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