38 results about "Brassica carinata" patented technology

The invention discloses a method for increasing proanthocyanidin content in escherichia coli by cotransformation of brassica juncea gene ANS and brassica carinata BAN. According to the invention, an anthocyanidin synthetase ANS gene is cloned from cDNA of brassica juncea seeds, and an anthocyanidin reductase BAN gene is cloned from cDNA of brassica carinata seeds; a prokaryotic expression vector pET-ANS-BAN containing the ANS and BAN genes is constructed; and the ANS and the BAN genes are introduced into a BL21 escherichia coli strain simultaneously. The transformed strain is cultured in an LB liquid medium at 28-38 DEG C at 150-250 rpm for 6-12 hours, transferred into an LB liquid medium containing grape skin extracts (0.2-0.8 g / L), and cultured under shaking at 28-38 DEG C at 150-250 rpm for 36-54 hours; the strain is collected by centrifugation; and the proanthocyanidin content in escherichia coli is determined by a DMACA-HCl method. The transgenic escherichia coli obtained in the invention has significantly increased proanthocyanidin content, and the maximum content can be 24.2 times higher than that of a non-transformed control strain.

The invention discloses a method for increasing proanthocyanidin content in escherichia coli by cotransformation of brassica juncea gene ANS and brassica carinata BAN. According to the invention, an anthocyanidin synthetase ANS gene is cloned from cDNA of brassica juncea seeds, and an anthocyanidin reductase BAN gene is cloned from cDNA of brassica carinata seeds; a prokaryotic expression vector pET-ANS-BAN containing the ANS and BAN genes is constructed; and the ANS and the BAN genes are introduced into a BL21 escherichia coli strain simultaneously. The transformed strain is cultured in an LB liquid medium at 28-38 DEG C at 150-250 rpm for 6-12 hours, transferred into an LB liquid medium containing grape skin extracts (0.2-0.8 g / L), and cultured under shaking at 28-38 DEG C at 150-250 rpm for 36-54 hours; the strain is collected by centrifugation; and the proanthocyanidin content in escherichia coli is determined by a DMACA-HCl method. The transgenic escherichia coli obtained in the invention has significantly increased proanthocyanidin content, and the maximum content can be 24.2 times higher than that of a non-transformed control strain.

The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically relating to Brassica carinata variety DH-20.141. The present invention relates to seeds, plants or parts thereof, cells, methods of making, and uses of this variety and its progeny.

The invention discloses a molecular marker and a method for identifying hybrids between Chinese cabbage and Ethiopian mustard and tracking the chromosomal segregation of A10 and C09 offspring materials, belonging to the field of plant genetics and breeding. This marker is a pair of co-dominant molecular markers, which can be used to identify the authenticity of hybrids between Chinese cabbage and Ethiopian mustard, and can also be used to identify and track self-crossed offspring, backcrossed offspring, chromosome addition lines, and recombination of distant hybrids Chromosome segregation of A10 and C09 in segregating populations and plants.

The invention discloses a molecular marker and a method for identifying hybrids between Chinese cabbage and Ethiopian mustard and tracking the chromosomal separation of A03 and C03 offspring materials, belonging to the field of plant genetics and breeding. This marker is a pair of co-dominant molecular markers, which can be used to identify the authenticity of hybrids between Chinese cabbage and Ethiopian mustard, and can also be used to identify and track self-crossed offspring, backcrossed offspring, chromosome addition lines, and recombination of distant hybrids Chromosomal segregation of A03 and C03 in segregating populations and plants.

The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically relating to Brassica carinata variety AGR044-312E. The present invention relates to seeds, plants or parts thereof, cells, methods of making, and uses of this variety and its progeny.