31 results about "Fatty acid elongases" patented technology

The present invention relates to a fungal C16 / 18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C16 / 18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16 / 18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.

This document provides materials and methods for generating oilseed (e.g., pennycress) plants that having low levels of erucic acid. For example, oilseed plants having reduced expression levels of one or more polypeptides involved in erucic acid metabolism (e.g., fatty acid elongase 1 (FAE1)), as well as materials and methods for making and using oilseed plants having low levels of erucic acid are provided.

The invention discloses an engineered yeast strain producing nervonic acid. The yeast strain overexpresses genes related to enzymes required for the synthesis of long-chain unsaturated fatty acids such as fatty acid elongase, desaturase, and diglyceride acyltransferase, and any Optionally, the triglyceride synthesis and decomposition pathway, the sphingomyelin synthesis and decomposition pathway, the lipid subcellular level synthesis and decomposition pathway, and the redox balance pathway of the strain are further regulated. The recombinant yeast strain can produce microbial oil, and the content of the prepared nervonic acid accounts for 39.6% of the total fatty acid content.

Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds of leaves.

The invention belongs to the technical field of genetic engineering and particularly relates to dsRNA of a migratory locust fatty acid elongase gene LmElo and a preparation method and application of the dsRNA. The preparation method of the dsRNA of the migratory locust fatty acid elongase gene LmElo comprises the following steps of a, designing an upstream primer sequence and a downstream primer sequence and synthesizing the upstream primer sequence and the downstream primer sequence; b, combining with upstream and downstream primers for designing the migratory locust fatty acid elongase geneLmElo, obtaining a full-length fragment of the fatty acid elongase gene through PCR amplification, purifying an obtained product, cloning and converting the product into escherichia coli, performing sequencing, conducting verification and obtaining the full-length nucleotide sequence of the gene, wherein the nucleotide sequence is shown as SEQ ID NO:1; c, designing upstream and downstream primersof the dsRNA on the basis of the sequence of the migratory locust fatty acid elongase gene which is shown as SEQ ID NO:1, wherein the sequences of the upstream and downstream primers are shown as SEQID NO:3 and SEQ ID NO:4 respectively, and the upstream primer and the downstream primer both carry a T7 promoter subsequence; d, carrying out transcriptive synthesis of the dsRNA.

The present disclosure provides methods and compositions for modifying fatty acids in Camelina sativa oil. Fatty Acid Desaturase 2 (FAD2), Fatty Acid Desaturase 3 (FADS), and / or Fatty Acid Elongase 1 (FAE1) genes regulate fatty acid composition in camelina oil.

This invention relates to nucleic acid sequences coding for a Lunaria annua, Cardamine graeca or Teesdalia nudicaulis fatty acid elongase, yeast cells expressing the genes / enzymes, plants themselves and cells of such plants and seeds which contain a heterologous gene coding for a L. annua, C. graeca or T. nudicaulis fatty acid elongase gene, the plant or seed being capable of producing increased proportion of a very long chain monounsaturated fatty acid, especially nervonic acid and eicosenoic acid, beyond that of a control plant or seed lacking the heterologous FAE gene or genes.

The invention provides a rocket salad fatty acid elongase and a coding gene EvFAE1 thereof. The gene is introduced into yeast, and the content of recombination yeast erucic acid reaches 2.50+ / -0.06%. The protein and coding gene have important theoretical and practical significance for studying a plant erucic acid genetic regulation mechanism, and improving the erucic acid content of plants and correlated characters, plays an important role in the erucic acid genetic engineering improvement and is wide in the application prospect.

The invention relates to a DNA sequence for coding Myrmecia incisa delta-6 fatty acid elongase, which comprises: a, the nucleotide sequence in SEQIDNO.10; or b, the nucleotide sequence complementary to the nucleotide sequence in a. The invention also provides a recombinant expression vector and genetically engineered host cells of the nucleotide sequence and the application thereof. The advantages of the invention are as follows: it is testified through the molecular cloning and functional identification of the Myrmecia incisa delta-6 fatty acid elongase gene that the gene code is delta-6 fatty acid elongase; the application provided by the invention provides a theoretical basis for improving the capability of long-chain polyunsaturated fatty acid synthesis from oil crops, and theoretically, the gene provided by the invention can be introduced into oil crops to effectively prolong the precursors that are necessary for the synthesis of arachidonic acid such as gamma-linolenic acid and endow the oil crops with the capability of the long-chain polyunsaturated fatty acid synthesis so as to improve the quality of the oil crops.

The invention discloses a method for increasing the palmitoleic acid content of Saccharomyces cerevisiae. The present invention provides recombinant bacteria, in order to carry out the following 1) transformation in the oil-producing Saccharomyces cerevisiae to obtain the recombinant bacteria: 1) the recombinant bacteria obtained by improving the transcriptional activity of the OLE1 gene in the oil-producing Saccharomyces cerevisiae. In the present invention, in the Saccharomyces cerevisiae strain YS58 that produces more oil, the transcription factor Mga2 or the truncated Mga2 gene fragment is overexpressed, the expression level of the OLE1 gene regulating Δ9 desaturase is increased, and the content of palmitoleic acid in Saccharomyces cerevisiae is increased, Simultaneously express the exogenous fatty acid elongase genes KCS and FAE to construct Saccharomyces cerevisiae genetically engineered bacteria and increase the content of palmitoleic acid in Saccharomyces cerevisiae.

The invention provides a fatty acid elongase derived from brassicealongata ehrhart, and a coding gene thereof (BeFAE1). The gene is introduced in to yeast; and the erucic acid content in the recombinant yeast reaches 1.52+ / -0.13%. The protein and the coding gene thereof have important theoretical and practical significance for the research of a genetic control mechanism of plant erucic acids, the increasement of the erucic acid content and the improvement of related traits, can play an important role in the genetic engineering improvement of the plant erucic acid genes, and have wide application prospects.

Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds or leaves.

The invention discloses a rainbow trout fatty acid elongase gene named as an OmElo gene. The nucleotide sequence of the rainbow trout fatty acid elongase gene is SEQ ID No.1. The OmElo gene is the novel elongase gene obtained after a source gene is optimized and designed according to the preference of encoding gene expression sequential codons in silkworm genome sequential data. The amino acid sequence of the protein encoded by guidance of the OmElo gene is SEQ ID No.2. The invention further provides a recombinant expression vector comprising the OmElo gene and a construction method thereof. A promoter of an expression cassette of the recombinant expression vector is the BmNPV very-early promoter IE1. The recombinant expression vector comprising OmElo gene can be applied to obtaining a silkworm transgenic strain with the high content of cis-11,14,17-epoxyeicosatrienoic acid. The invention further provides a method for improving the content of the cis-11,14,17-epoxyeicosatrienoic acid in silkworm tissue, the rainbow trout fatty acid elongase OmElo gene can be guided into a silkworm embryo, and screening is performed to obtain the target silkworm transgenic strain.

The invention belongs to the technical field of genetic engineering, and in particular relates to a dsRNA of migratory locust fatty acid synthase gene LmFAS, a preparation method and application thereof. The preparation method of the dsRNA of migratory locust fatty acid synthase gene LmFAS of the present invention, comprises the following steps: a, design upstream primer sequence and downstream primer sequence, and synthesize; B, extract RNA and reverse transcribe into first-strand cDNA as The template, combined with the upstream and downstream primers of the fatty acid synthase gene LmFAS of migratory locusts, was amplified by PCR to obtain the full-length fragment of the fatty acid elongase gene, the obtained product was purified, connected to the T4 vector, and then transformed into Trans1‑T1 competent cells Medium culture, spot picking, detection, and sequencing; c, based on the fatty acid synthase gene sequence SEQ ID NO: 1 of migratory locust, design dsRNA upstream and downstream primers, the sequences of which are SEQ ID NO: 3 and SEQ ID NO: 4, the upper Downstream primers all carry T7 promoter sequence; d, dsRNA is transcribed and synthesized.

Fatty acids, and methods for the production thereof, are provided. Transgenic organisms, microbes, plants, seeds, and cells useful in the production of fatty acids, along with related expression vectors, phages, plasmids, nucleic acids, and enzymes, are also provided. Methods for the production of fatty acids such as docosadienoic acid and docosatrienoic acid, involving the use of winter aconite (Eranthis hyemalis) EhELO1 elongase, are described in detail.