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Cabbage-type rape TT8 gene families and applications thereof

A technology of Brassica napus and genes, which is applied in the field of genetic engineering and achieves good results in application prospects

Inactive Publication Date: 2013-03-20
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports on the number of members, protein characteristics, evolutionary relationship, tissue specificity of expression, relationship between traits such as yellow seeds, and transgenic molecular breeding of TT8 gene in Brassica species such as Brassica napus, Chinese cabbage, and Brassica oleracea.

Method used

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  • Cabbage-type rape TT8 gene families and applications thereof
  • Cabbage-type rape TT8 gene families and applications thereof
  • Cabbage-type rape TT8 gene families and applications thereof

Examples

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Embodiment Construction

[0040] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0041] The plant materials used in the preferred embodiment: the cabbage materials are all from the Brassica napus subspecies (B.rapa ssp.oleifera), including the typical black seed line 06K130 and the yellow seed line 06K124; the cabbage materials are all from the kale variety (B. oleracea var.acephala), including typical black seed line 06K158 and yellow seed line 06K165; Brassica napus materials include typical black seed line 5B, black seed variety Xiangyou 15, near-isogenic black seed line L1...

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Abstract

The invention discloses cabbage-type rape as well as parent species Chinese cabbage and cabbage TT8 gene families and applications thereof, wherein, the Chinese cabbage TT8 gene family comprises a BrTT8-1 gene (SEQ ID No.1-2) and a Br TT8-2 gene (SEQ ID No.3-4); the cabbage TT8 gene family comprises a BoTT8-1 gene (SEQ ID No.5-6) and a BoTT8-2 gene (SEQ ID No.7-8); the cabbage-type rape TT8 gene family comprises a BnTT8-1 gene (SEQ ID No.9-10) and a BnTT8-2 gene (SEQ ID No.11-12). The above gene families can be used for molecule breeding of the brassica crop seed properties.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to Brassica napus and its parent species Chinese cabbage (Brassica rapa) and cabbage (Brassica oleracea) TT8 (TRANSPARENT TESTA8, transparent seed coat 8; also known as bHLH42, BASIC HELIX- LOOP-HELIX42, basic helix-loop-helix 42) gene family and its application. Background technique [0002] The genus Brassica (Brassica) of the Brassicaceae (Brassicaceae) includes many oil crops, vegetables and ornamental plant species. It provides humans with nutritious edible oils, vegetables and ornamental plants, and provides feed for animal husbandry. It has an important economic value. Among the Brassica species, the allotetraploid species Brassica napus was formed from two diploid species Brassica oleracea and Brassica oleracea through interspecific hybridization followed by remultiplication. Brassica napus is the second largest oil crop in the world, widely planted all over t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
Inventor 柴友荣殷家明赵文军谢伶俐肖霞李加纳林呐张瑞熙许本波
Owner SOUTHWEST UNIV
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