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Microbial secondary metabolite palmatine hydrochloride and application thereof

A secondary metabolite, palmatine hydrochloride technology, applied in the direction of microorganisms, microorganism-based methods, applications, etc., can solve problems that have not been seen, and achieve excellent results, strong pH adaptability, and strong adaptability

Inactive Publication Date: 2015-04-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the literature, there is no public report on the use of palmatine hydrochloride, a secondary metabolite of the antibiotic Lysobacterium, to control bacterial diseases of rice and clubroot of cruciferous vegetables

Method used

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  • Microbial secondary metabolite palmatine hydrochloride and application thereof
  • Microbial secondary metabolite palmatine hydrochloride and application thereof
  • Microbial secondary metabolite palmatine hydrochloride and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: the stability test test of palmatine hydrochloride

[0026] 1. Test materials:

[0027] Tested strains: virulent strain YM15 of rice bacterial leaf spot, virulent strain FUJ of rice bacterial blight, antibiotic lysobacter strain YFY 02; test preparation: palmatine hydrochloride; test medium: KB medium, NA medium; test agents: sodium hydroxide, hydrochloric acid; test instruments: pH meter, water bath, ultraviolet lamp, sterile workbench, ultraviolet / visible light spectrophotometer.

[0028] 2. Test method

[0029] 2.1 Determination of the tolerance of palmatine hydrochloride to pH, temperature and ultraviolet rays

[0030] The preparation of palmatine hydrochloride refers to the detailed process of preparation of biological preparations. The test concentration of palmatine hydrochloride solution is 1ug / mL, and the amount added in the Oxford cup is 100uL / cup; the plate with bacteria is added 50uL 3×10 8 cfu / mL suspension of leaf spot fungus YM15 or bac...

Embodiment 2

[0042] Example 2: Field disease control test of palmatine hydrochloride on rice bacterial spot disease

[0043] 1. Test materials

[0044] Tested strains: virulent strain YM15 of rice bacterial leaf spot, antibiotic lysobacter strain YFY 02; Tested preparations: palmatine hydrochloride, 1% Shenzimycin suspension 1000 times liquid, Ye Banning 100 times liquid; The test medium: KB medium, NA medium; the test crops: the main local rice variety Deyou 13, the spot disease resistant variety Hongyou No. 4; the control object: rice bacterial streak disease;

[0045] Experimental location: Rice experimental field in Ruili City, Dehong Prefecture, Yunnan Province, with medium fertility, and the main rice variety-Deyou 13 planted in the land. The plot is a single-row area with an area of ​​30m 2 , each treatment set 3 replicates, randomly arranged, a total of 9 plots, surrounded by protection rows, protection behavior stripe spot disease resistant variety Hongyou No. 4, and standardized...

Embodiment 3

[0064] Embodiment 3: Field control test of palmatine hydrochloride on rice bacterial blight

[0065] 1. Test materials:

[0066] Test strains: rice bacterial blight virulent strain FUJ, antibiotic lysobacter strain YFY 02; test preparations: palmatine hydrochloride preparation, 1% Shenzimycin suspension 1000 times liquid, Ye Banning 100 times liquid; The test medium: KB medium, NA medium; the test crops: the rice variety Deyou 13 planted by the landlord, the bacterial blight-resistant variety Hongyou No. 4; the control object: rice bacterial blight;

[0067] Experimental location: Rice experimental field in Ruili City, Dehong Prefecture, Yunnan Province, with medium fertility, and the main planted rice variety - Deyou 13. The plot is a single row area with an area of ​​30m 2 , each treatment set 3 replicates, randomly arranged, a total of 9 plots, surrounded by protection rows, protection behavior of bacterial blight resistant variety Hongyou No. 4, standardized management ac...

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Abstract

The invention relates to a microbial secondary metabolite palmatine hydrochloride and application thereof and belongs to the technical field of biological pesticides. The secondary metabolite is prepared by the following steps: (a), producing a strain of L.antibioticus YFY02 with the collection number of CGMCC NO.2403; (b) culturing the strain YFY02 for two days by utilizing a KB culture medium, performing enlarged culture by adopting a conventional fermentation method, extracting the fermentation liquor by using ethyl acetate, and performing rotary evaporation, thereby obtaining a crude metabolite extract; (c) by utilizing liquid chromatography, Sephadex LH-20 gel column chromatography, mass spectrometry, nuclear magnetic resonance and other spectroscopic techniques, and separating, purifying and identifying the crude extract of the secondary metabolite; and (d) identifying to obtain the effective secondary metabolite palmatine hydrochloride. The invention also discloses application of the microbial secondary metabolite palmatine hydrochloride in the preparation of agents for preventing and controlling bacterial blight of rice, bacterial leaf streak and plasmodiophora brassicae woronin. The microbial secondary metabolite disclosed by the invention has the advantages of environment friendliness, low cost and excellent effects of preventing and controlling the rice bacterial diseases and plasmodiophora brassicae woronin.

Description

Technical field: [0001] The invention relates to a microbial secondary metabolite palmatine hydrochloride and its application, belonging to the technical field of microbial pesticide production. Background technique: [0002] Rice bacterial streak disease, caused by Xanthomonoas oryzae pv. oryzicola, is widely distributed in tropical and subtropical regions of Asia. area, has become one of the main diseases in the southern rice areas of my country. When the disease occurs, it spreads rapidly, causing rice leaves to turn yellow and die, empty grains to increase, and yield to decrease. When it is severe, it can reach 40-60%. This disease seriously threatens rice production. [0003] Rice bacterial blight, caused by the infection of Xanthomonas oryzae pv. Serious; except for Xinjiang and Northeast China, the disease has always been an important disease affecting the safe production of rice in my country. Rice bacterial blight has occurred in China for more than 70 years, and i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18A01N43/90A01P3/00A01P1/00C12R1/01
Inventor 姬广海周丽洪魏兰芳
Owner YUNNAN AGRICULTURAL UNIVERSITY
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