Streptomyces luteogriseus YAAS0018 strain, and plasmodiophora brassicae resistant microbial preparation and application thereof
A technology of Streptomyces luteus and microbial preparations, applied in the direction of microorganisms, methods based on microorganisms, chemicals for biological control, etc., to achieve good application prospects, good control effects, and low raw material costs
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Embodiment 1
[0030] Example 1 The acquisition, identification, culture and preservation of Streptomyces luteogriseus YAAS0018CGMCC No. 7628
[0031] 1. Acquisition and identification of Streptomyces luteogriseus YAAS0018CGMCC No. 7628
[0032] 1.1 Material
[0033] 1.1 Source
[0034] Collected from the soil of Niuluoshan tea plantation in Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, China.
[0035] 1.2 Medium
[0036] Medium formula: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO4·7H 2 O0.5g, NaCl0.5g, FeSO4·7H 2 O0.01g, agar 20g, pH=7.5.
[0037] 1.3 method
[0038] Weigh 10 g of soil sample, put it into an Erlenmeyer flask containing 100 mL of sterile water, and add 10 drops of 10% phenol to inhibit bacterial growth. Shake for 10 minutes to make 10 -1 Bacteria suspension. According to the serial dilution separation method, 10 -3 Bacteria suspension. Pipette 0.1mL10 -3 Inject the bacterial suspension onto the plate medium, use a sterile glass spatula to spread the bacterial suspen...
Embodiment 2
[0050] Example 2: Greenhouse pot experiment for preventing and controlling root swelling of cabbage
[0051] (1) Anti-cruciferous root edema microorganism preparation and preparation method thereof
[0052] 1. The preparation method of microbial preparations against cruciferous root edema, the steps are as follows:
[0053] (1) Test tube species cultivation
[0054] The Streptomyces luteogriseus YAAS0018CGMCC No. 7628 strain was inoculated on the test tube solid medium slope and cultured at 28°C for 7 days to obtain test tube species. The formula of the test tube solid culture medium is: yeast extract 4g, glucose 4g, malt extract 5g, vitamin B 1 1mg, vitamin B 6 1mg, riboflavin 1mg, niacin 1mg, phenylalanine 1mg, biotin 1mg, alanine 0.3mg, FeSO 4 ·7H 2 O20mg, MnCl 2 ·2H 2 O10mg, ZnSO 4 ·7H 2 O10mg, 20g agar, 1000ml water, pH7.8.
[0055] (2) Liquid seed culture
[0056] Use triangular flask shake flask culture, the method is:
[0057] ① Prepare a liquid seed culture medium, and mix the i...
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