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Streptomyces luteogriseus YAAS0018 strain, and plasmodiophora brassicae resistant microbial preparation and application thereof

A technology of Streptomyces luteus and microbial preparations, applied in the direction of microorganisms, methods based on microorganisms, chemicals for biological control, etc., to achieve good application prospects, good control effects, and low raw material costs

Inactive Publication Date: 2013-09-11
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the successful application of biological agents in field control of cruciferous clubroot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The acquisition, identification, culture and preservation of Streptomyces luteogriseus YAAS0018CGMCC No. 7628

[0031] 1. Acquisition and identification of Streptomyces luteogriseus YAAS0018CGMCC No. 7628

[0032] 1.1 Material

[0033] 1.1 Source

[0034] Collected from the soil of Niuluoshan tea plantation in Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, China.

[0035] 1.2 Medium

[0036] Medium formula: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO4·7H 2 O0.5g, NaCl0.5g, FeSO4·7H 2 O0.01g, agar 20g, pH=7.5.

[0037] 1.3 method

[0038] Weigh 10 g of soil sample, put it into an Erlenmeyer flask containing 100 mL of sterile water, and add 10 drops of 10% phenol to inhibit bacterial growth. Shake for 10 minutes to make 10 -1 Bacteria suspension. According to the serial dilution separation method, 10 -3 Bacteria suspension. Pipette 0.1mL10 -3 Inject the bacterial suspension onto the plate medium, use a sterile glass spatula to spread the bacterial suspen...

Embodiment 2

[0050] Example 2: Greenhouse pot experiment for preventing and controlling root swelling of cabbage

[0051] (1) Anti-cruciferous root edema microorganism preparation and preparation method thereof

[0052] 1. The preparation method of microbial preparations against cruciferous root edema, the steps are as follows:

[0053] (1) Test tube species cultivation

[0054] The Streptomyces luteogriseus YAAS0018CGMCC No. 7628 strain was inoculated on the test tube solid medium slope and cultured at 28°C for 7 days to obtain test tube species. The formula of the test tube solid culture medium is: yeast extract 4g, glucose 4g, malt extract 5g, vitamin B 1 1mg, vitamin B 6 1mg, riboflavin 1mg, niacin 1mg, phenylalanine 1mg, biotin 1mg, alanine 0.3mg, FeSO 4 ·7H 2 O20mg, MnCl 2 ·2H 2 O10mg, ZnSO 4 ·7H 2 O10mg, 20g agar, 1000ml water, pH7.8.

[0055] (2) Liquid seed culture

[0056] Use triangular flask shake flask culture, the method is:

[0057] ① Prepare a liquid seed culture medium, and mix the i...

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Abstract

The invention discloses a streptomyces luteogriseus YAAS0018 strain, and a plasmodiophora brassicae resistant microbial preparation and an application thereof, and belongs to the technical field of microbial pesticides. The strain is the streptomyces luteogriseus YAAS0018 CGMCC (China General Microbiological Culture Collection Center) No.7628. The plasmodiophora brassicae resistant microbial preparation of the strain is prepared by strain tube seed culture, liquid seed culture and liquid fermentation culture. The strain and the plasmodiophora brassicae resistant microbial preparation which contains the strain have an obvious prevention effect on the plasmodiophora brassicae; and the average prevention and treatment effect is up to 91.71 percent.

Description

Technical field [0001] The invention relates to a strain of Streptomyces luteus YAAS0018 and its anti-cruciferous root edema microbial preparation and application thereof, belonging to the technical field of microbial pesticides. Background technique [0002] The pathogenic bacteria that cause root swelling in the cruciferous family belong to the flagellum subphylum, Rhizotocepha, and the biflagellate fungus of the genus Rhizoctonia. The disease was first reported in the United States in 1852. It was widespread in the former Soviet Union in the 19th century and caused devastating disasters. In 1975, Soviet scientist Woronin identified this pathogen and named it "Plasmodiophora brassicae". In recent years, the disease has become more and more serious worldwide, widely distributed in North America, South America, Europe, South Africa, East Asia and other places. my country has been reported in Taiwan as early as 1936. Currently, it is mainly distributed in Heilongjiang, Liaoning,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/465
Inventor 杨佩文杨明英曹继芬李向东曾莉毕云青裴卫华赵志坚李元广周丽凤
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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