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Breeding method for high oleic acid rape for resisting plasmodiophora brassicae and application of breeding method

A clubroot-resistant, high-oleic acid technology, applied in the fields of application, botany equipment and methods, biochemical equipment and methods, etc., can solve problems affecting the correct selection of disease-resistant materials, the process of breeding, and the incidence of inoculation , to achieve the effect of improving the economic efficiency and market competitiveness of the enterprise, increasing the market value and shortening the breeding period

Active Publication Date: 2019-10-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the process of the transformation of conventional rapeseed varieties resistant to clubroot, each generation needs to use the method of field inoculation of clubroot to identify the genotype of the intermediate material, and factors such as temperature, humidity, and field distribution of the pathogen will affect the incidence of inoculation , which in turn affects the correct selection of disease-resistant materials and the process of breeding

Method used

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  • Breeding method for high oleic acid rape for resisting plasmodiophora brassicae and application of breeding method
  • Breeding method for high oleic acid rape for resisting plasmodiophora brassicae and application of breeding method
  • Breeding method for high oleic acid rape for resisting plasmodiophora brassicae and application of breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Rapid Polymerization of High Oleic Acid and Clubroot Resistance Genes Using Molecular Markers

[0034] 1. Configure the F1 combination of ordinary oleic acid content and high oleic acid content

[0035] The excellent Brassica napus variety Zhongshuang 11 (the original breeding unit is the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences; the National Crop Variety Approval Committee, the National Approval Oil 2008030; the oleic acid content of the seeds of this variety was determined to be 68% in 2012) was selected as the parent This, and the Brassica napus line A A254 with high oleic acid content (the oleic acid content measured in 2012 was 78%, the seeds of this material have been sent to the Chinese typical culture in Wuhan University, Wuhan City, Hubei Province on November 20, 2009 Preserved in the Center for Preservation of Plants, its preservation number is CCTCC-P200909; and it has been disclosed in the Chinese Invention Paten...

Embodiment 2

[0057] Example 2 Backcross Generation Marker-Assisted Selection Screening Homozygous Disease-Resistant High-oleic High-quality Individual Plants

[0058] 1. Screen different additive materials for indoor inoculation identification: select mycorrhizae of clubroot plants in Zhijiang area, grind them and mix them with nutrient soil to form fungus soil. Then it was sown indoors to investigate its root incidence and identify its resistance to clubroot. The incidence rate of control (parent) materials in each group was 100%, and the incidence rate of disease-resistant materials was 0%. Some test results such as figure 2 As shown, the test results show that this method can effectively detect the resistance of disease-resistant materials to pathogenic sources.

[0059] 2. Use the cnu_SSR492 primer to screen and identify the target gene of different strains in the BC2F2 population (with different source backgrounds of recurrent parents) through the PAGE gel detection method. The spe...

Embodiment 3

[0076] Embodiment 3 CRb disease-resistant site field resistance identification-marker selection effect

[0077] The clubroot resistant high oleic strain screened in Example 2 was planted in Jingmen, Hubei and Huangshan, Anhui, two fields with severe clubroot. Three repetitions were set up in the experiment, and three control varieties were planted in each repetition. Genotype sampling identification and field resistance investigation were carried out in mid-December of the same year.

[0078] clubroot disease classification standard

[0079] Level 0: no symptoms of disease;

[0080] Grade 1: There are only a few and small nodules on the tip of the fibrous roots;

[0081] Grade 2: nodules appear on the lateral roots;

[0082] Grade 3: large nodules on fibrous roots and lateral roots and small nodules at the end of the main root;

[0083] Grade 4: The main root is all swollen, the clubroot part on the root begins to crack and rot, and the plant shows wilting symptoms or dies...

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Abstract

The invention belongs to the technical field of rape breeding, and particularly relates to a breeding method for high oleic acid rape for resisting plasmodiophora brassicae and an application of the breeding method. The breeding method is characterized by comprising the following steps of enabling a high oleic acid plant and a plasmodiophora brassicae-resisting gene containing plant to be hybridized to obtain composite F1 rape seeds containing high oleic acid and CRb disease-resistant sites; planting the composite F1 rape seeds, identifying the relevant gene sites with high oleic acid and a plasmodiophora brassicae resisting molecular marker, and selecting individuals containing the sites and recurrent parents for hybridizing; performing high oleic acid and plasmodiophora brassicae resisting molecular marker analysis on backcross and selfed progeny groups, performing screening, and performing homozygosis to obtain the high oleic acid plasmodiophora brassicae resisting plant. The invention provides a simple, quick and effective method for breeding good disease-resistant high oleic acid rape kinds. The breeding method can selectively select a single plant according to the genotype atany stage in the breeding process, so that the selection efficiency is improved, and the breeding age limit is shortened.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a method for breeding rapeseed with high oleic acid resistance to clubroot and its application. More specifically, it relates to a method of using clubroot disease indoor inoculation identification and molecular marker-assisted selection to breed superior clubroot disease-resistant high-oleic rapeseed rape strains. Background technique [0002] The composition of fatty acids in rapeseed directly determines the edible quality and nutritional value of rapeseed oil. Oleic acid is an octadecyl monounsaturated fatty acid. The oleic acid content in common rape seeds is 17-65%. The high oleic rapeseed in the present invention refers to the rapeseed varieties and the derived rapeseed oils whose oleic acid ratio in seeds and rapeseed oil fatty acids is greater than 75%. [0003] High oleic edible oils, including high oleic rapeseed oil, have high application value ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周永明张椿雨付蓉张莉莉黄会斌范楚川
Owner HUAZHONG AGRI UNIV
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